Dietary n-6:n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoa
This study was aimed to evaluate the effects of dietary n-6:n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6:n-3 ratios (14.4 and 6.6) and two Vitamin E levels...
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doaj-a79a1270f40e409da8452627e7a669882020-11-24T22:01:46ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622017-01-0119222322910.4103/1008-682X.170446Dietary n-6:n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoaQing LiuYuan-Fei ZhouRun-Jia DuanHong-Kui WeiJian PengSi-Wen JiangThis study was aimed to evaluate the effects of dietary n-6:n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6:n-3 ratios (14.4 and 6.6) and two Vitamin E levels (200 and 400 mg kg−1 ). During 16 weeks of treatment, fresh semen was collected at weeks 0, 8, 12, and 16 for measurements of motility characteristics, contents of fatty acids, membrane properties (membrane fluidity and membrane integrity), and lipid peroxidation of the spermatozoa. The semen was diluted in Beltsville Thawing Solution (BTS) extender and stored at 17°C, and the sperm motility was assessed at 12, 36, 72, and 120 h of storage. The 6.6 n-6:n-3 dietary ratio increased the contents of n-3 polyunsaturated fatty acids (PUFAs) and docosahexaenoic acid (DHA) and improved the membrane integrity and membrane fluidity of the spermatozoa, resulting in notably increased total motility, sperm progressive motility, and velocity parameters of fresh semen. Feeding diet with Vitamin E (400 mg kg−1 ) prevented sperm lipid peroxidation, and resulted in higher total motility and sperm progressive motility in fresh and liquid stored semen. In conclusion, the adjustment of n-6:n-3 ratio (6.6) and supply of Vitamin E (400 mg kg−1 ) successfully improved sperm motility characteristics and thus may be beneficial to the fertility of boars, which might be due to the modification of the physical and functional properties of spermatozoa membrane in response to dietary supplementation.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2017;volume=19;issue=2;spage=223;epage=229;aulast=Liuboar; dietary n-6:n-3 ratio; fatty acid composition; lipid peroxidation; membrane properties; motility characteristics; Vitamin E |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qing Liu Yuan-Fei Zhou Run-Jia Duan Hong-Kui Wei Jian Peng Si-Wen Jiang |
spellingShingle |
Qing Liu Yuan-Fei Zhou Run-Jia Duan Hong-Kui Wei Jian Peng Si-Wen Jiang Dietary n-6:n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoa Asian Journal of Andrology boar; dietary n-6:n-3 ratio; fatty acid composition; lipid peroxidation; membrane properties; motility characteristics; Vitamin E |
author_facet |
Qing Liu Yuan-Fei Zhou Run-Jia Duan Hong-Kui Wei Jian Peng Si-Wen Jiang |
author_sort |
Qing Liu |
title |
Dietary n-6:n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoa |
title_short |
Dietary n-6:n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoa |
title_full |
Dietary n-6:n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoa |
title_fullStr |
Dietary n-6:n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoa |
title_full_unstemmed |
Dietary n-6:n-3 ratio and Vitamin E improve motility characteristics in association with membrane properties of boar spermatozoa |
title_sort |
dietary n-6:n-3 ratio and vitamin e improve motility characteristics in association with membrane properties of boar spermatozoa |
publisher |
Wolters Kluwer Medknow Publications |
series |
Asian Journal of Andrology |
issn |
1008-682X 1745-7262 |
publishDate |
2017-01-01 |
description |
This study was aimed to evaluate the effects of dietary n-6:n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6:n-3 ratios (14.4 and 6.6) and two Vitamin E levels (200 and 400 mg kg−1 ). During 16 weeks of treatment, fresh semen was collected at weeks 0, 8, 12, and 16 for measurements of motility characteristics, contents of fatty acids, membrane properties (membrane fluidity and membrane integrity), and lipid peroxidation of the spermatozoa. The semen was diluted in Beltsville Thawing Solution (BTS) extender and stored at 17°C, and the sperm motility was assessed at 12, 36, 72, and 120 h of storage. The 6.6 n-6:n-3 dietary ratio increased the contents of n-3 polyunsaturated fatty acids (PUFAs) and docosahexaenoic acid (DHA) and improved the membrane integrity and membrane fluidity of the spermatozoa, resulting in notably increased total motility, sperm progressive motility, and velocity parameters of fresh semen. Feeding diet with Vitamin E (400 mg kg−1 ) prevented sperm lipid peroxidation, and resulted in higher total motility and sperm progressive motility in fresh and liquid stored semen. In conclusion, the adjustment of n-6:n-3 ratio (6.6) and supply of Vitamin E (400 mg kg−1 ) successfully improved sperm motility characteristics and thus may be beneficial to the fertility of boars, which might be due to the modification of the physical and functional properties of spermatozoa membrane in response to dietary supplementation. |
topic |
boar; dietary n-6:n-3 ratio; fatty acid composition; lipid peroxidation; membrane properties; motility characteristics; Vitamin E |
url |
http://www.ajandrology.com/article.asp?issn=1008-682X;year=2017;volume=19;issue=2;spage=223;epage=229;aulast=Liu |
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