Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems
Intracellular protein gradients are significant determinants of spatial organization. However, little is known about how protein patterns are established, and how their positional information directs downstream processes. We have accomplished the reconstitution of a protein concentration gradient th...
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eLife Sciences Publications Ltd
2014-10-01
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| Online Access: | https://elifesciences.org/articles/03949 |
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doaj-a7a155c7b8d945028f4c8bf8c1c537522021-05-04T23:28:15ZengeLife Sciences Publications LtdeLife2050-084X2014-10-01310.7554/eLife.03949Reconstitution of self-organizing protein gradients as spatial cues in cell-free systemsKatja Zieske0Petra Schwille1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Munich, GermanyDepartment of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Munich, GermanyIntracellular protein gradients are significant determinants of spatial organization. However, little is known about how protein patterns are established, and how their positional information directs downstream processes. We have accomplished the reconstitution of a protein concentration gradient that directs the assembly of the cell division machinery in E.coli from the bottom-up. Reconstituting self-organized oscillations of MinCDE proteins in membrane-clad soft-polymer compartments, we demonstrate that distinct time-averaged protein concentration gradients are established. Our minimal system allows to study complex organizational principles, such as spatial control of division site placement by intracellular protein gradients, under simplified conditions. In particular, we demonstrate that FtsZ, which marks the cell division site in many bacteria, can be targeted to the middle of a cell-like compartment. Moreover, we show that compartment geometry plays a major role in Min gradient establishment, and provide evidence for a geometry-mediated mechanism to partition Min proteins during bacterial development.https://elifesciences.org/articles/03949protein gradientFtsZcytoskeletoncell divisioncell-free reconstitutionmin protein |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Katja Zieske Petra Schwille |
| spellingShingle |
Katja Zieske Petra Schwille Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems eLife protein gradient FtsZ cytoskeleton cell division cell-free reconstitution min protein |
| author_facet |
Katja Zieske Petra Schwille |
| author_sort |
Katja Zieske |
| title |
Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems |
| title_short |
Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems |
| title_full |
Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems |
| title_fullStr |
Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems |
| title_full_unstemmed |
Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems |
| title_sort |
reconstitution of self-organizing protein gradients as spatial cues in cell-free systems |
| publisher |
eLife Sciences Publications Ltd |
| series |
eLife |
| issn |
2050-084X |
| publishDate |
2014-10-01 |
| description |
Intracellular protein gradients are significant determinants of spatial organization. However, little is known about how protein patterns are established, and how their positional information directs downstream processes. We have accomplished the reconstitution of a protein concentration gradient that directs the assembly of the cell division machinery in E.coli from the bottom-up. Reconstituting self-organized oscillations of MinCDE proteins in membrane-clad soft-polymer compartments, we demonstrate that distinct time-averaged protein concentration gradients are established. Our minimal system allows to study complex organizational principles, such as spatial control of division site placement by intracellular protein gradients, under simplified conditions. In particular, we demonstrate that FtsZ, which marks the cell division site in many bacteria, can be targeted to the middle of a cell-like compartment. Moreover, we show that compartment geometry plays a major role in Min gradient establishment, and provide evidence for a geometry-mediated mechanism to partition Min proteins during bacterial development. |
| topic |
protein gradient FtsZ cytoskeleton cell division cell-free reconstitution min protein |
| url |
https://elifesciences.org/articles/03949 |
| work_keys_str_mv |
AT katjazieske reconstitutionofselforganizingproteingradientsasspatialcuesincellfreesystems AT petraschwille reconstitutionofselforganizingproteingradientsasspatialcuesincellfreesystems |
| _version_ |
1721477011473432576 |