Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats
AIM: To investigate the effect of pomegranate juice (PJ) intake on overall oxidation status in retinas of diabetic rats. METHODS: Twenty-seven rats were divided into four groups as control (CO), diabetic (DM), control treated with PJ (CO-PJ), and diabetic treated with PJ (DM-PJ).The retina tissues...
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doaj-a7b5aae5ebc2403bb17371e17bd6dce12020-11-24T23:53:36ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982017-11-0110111662166810.18240/ijo.2017.11.05Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic ratsBetul Tugcu0Senay Asik Nacaroglu1Asuman Gedikbasi2Mehmet Uhri3Nur Acar4Hakan Ozdemir5Department of Ophthalmology, Faculty of Medicine, Bezmialem University, Istanbul 34093, TurkeyDepartment of Ophthalmology, Bagcilar Training and Research Hospital, Istanbul 34200, TurkeyDepartment of Biochemistry, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Istanbul 34147, TurkeyDepartment of Pathology, Kanuni Sultan Suleyman Training and Research Hospital, Istanbul 34303, TurkeyDepartment of Ophthalmology, Acibadem University, School of Medicine, Istanbul 34457, TurkeyDepartment of Ophthalmology, Faculty of Medicine, Bezmialem University, Istanbul 34093, TurkeyAIM: To investigate the effect of pomegranate juice (PJ) intake on overall oxidation status in retinas of diabetic rats. METHODS: Twenty-seven rats were divided into four groups as control (CO), diabetic (DM), control treated with PJ (CO-PJ), and diabetic treated with PJ (DM-PJ).The retina tissues were used to determine 8-hydroxy-2’-deoxyguanosine (8OHdG), malondialdehyde (MDA), reduced glutathione (GSH) levels, and the enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). RESULTS: The levels of 8OHdG and MDA were significantly increased in the retina of DM group compared to CO group (P=0.001, P<0.001 respectively). Both 8OHdG and MDA levels were decreased in PJ-DM group compared to DM group (P=0.004, P<0.001 respectively). The activities of antioxidant enzymes GSH, SOD, and GDH-Px were significantly decreased in the retina of DM group compared to CO group (P≤0.01). GSH and GSH-Px activities were higher in PJ-DM group compared with DM group (P=0.010, P=0.042, respectively) but SOD activity was not statistically different (P=0.938). CONCLUSION: PJ intake is found to be effective in decreasing oxidative end products, and in increasing the activities of antioxidant enzymes in diabetic retinas of rats, which suggests it may be effective against oxidative stress in diabetic retinas.http://www.ijo.cn/en_publish/2017/11/20171105.pdf1668antioxidant enzymesdiabetic retinopathyimmunohistochemical stainingoxidative stresspomegranate juice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Betul Tugcu Senay Asik Nacaroglu Asuman Gedikbasi Mehmet Uhri Nur Acar Hakan Ozdemir |
spellingShingle |
Betul Tugcu Senay Asik Nacaroglu Asuman Gedikbasi Mehmet Uhri Nur Acar Hakan Ozdemir Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats International Journal of Ophthalmology 1668 antioxidant enzymes diabetic retinopathy immunohistochemical staining oxidative stress pomegranate juice |
author_facet |
Betul Tugcu Senay Asik Nacaroglu Asuman Gedikbasi Mehmet Uhri Nur Acar Hakan Ozdemir |
author_sort |
Betul Tugcu |
title |
Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats |
title_short |
Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats |
title_full |
Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats |
title_fullStr |
Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats |
title_full_unstemmed |
Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats |
title_sort |
protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
International Journal of Ophthalmology |
issn |
2222-3959 2227-4898 |
publishDate |
2017-11-01 |
description |
AIM: To investigate the effect of pomegranate juice (PJ) intake on overall oxidation status in retinas of diabetic rats.
METHODS: Twenty-seven rats were divided into four groups as control (CO), diabetic (DM), control treated with PJ (CO-PJ), and diabetic treated with PJ (DM-PJ).The retina tissues were used to determine 8-hydroxy-2’-deoxyguanosine (8OHdG), malondialdehyde (MDA), reduced glutathione (GSH) levels, and the enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px).
RESULTS: The levels of 8OHdG and MDA were significantly increased in the retina of DM group compared to CO group (P=0.001, P<0.001 respectively). Both 8OHdG and MDA levels were decreased in PJ-DM group compared to DM group (P=0.004, P<0.001 respectively). The activities of antioxidant enzymes GSH, SOD, and GDH-Px were significantly decreased in the retina of DM group compared to CO group (P≤0.01). GSH and GSH-Px activities were higher in PJ-DM group compared with DM group (P=0.010, P=0.042, respectively) but SOD activity was not statistically different (P=0.938).
CONCLUSION: PJ intake is found to be effective in decreasing oxidative end products, and in increasing the activities of antioxidant enzymes in diabetic retinas of rats, which suggests it may be effective against oxidative stress in diabetic retinas. |
topic |
1668 antioxidant enzymes diabetic retinopathy immunohistochemical staining oxidative stress pomegranate juice |
url |
http://www.ijo.cn/en_publish/2017/11/20171105.pdf |
work_keys_str_mv |
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