Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats

AIM: To investigate the effect of pomegranate juice (PJ) intake on overall oxidation status in retinas of diabetic rats. METHODS: Twenty-seven rats were divided into four groups as control (CO), diabetic (DM), control treated with PJ (CO-PJ), and diabetic treated with PJ (DM-PJ).The retina tissues...

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Main Authors: Betul Tugcu, Senay Asik Nacaroglu, Asuman Gedikbasi, Mehmet Uhri, Nur Acar, Hakan Ozdemir
Format: Article
Language:English
Published: Press of International Journal of Ophthalmology (IJO PRESS) 2017-11-01
Series:International Journal of Ophthalmology
Subjects:
Online Access:http://www.ijo.cn/en_publish/2017/11/20171105.pdf
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spelling doaj-a7b5aae5ebc2403bb17371e17bd6dce12020-11-24T23:53:36ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982017-11-0110111662166810.18240/ijo.2017.11.05Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic ratsBetul Tugcu0Senay Asik Nacaroglu1Asuman Gedikbasi2Mehmet Uhri3Nur Acar4Hakan Ozdemir5Department of Ophthalmology, Faculty of Medicine, Bezmialem University, Istanbul 34093, TurkeyDepartment of Ophthalmology, Bagcilar Training and Research Hospital, Istanbul 34200, TurkeyDepartment of Biochemistry, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Istanbul 34147, TurkeyDepartment of Pathology, Kanuni Sultan Suleyman Training and Research Hospital, Istanbul 34303, TurkeyDepartment of Ophthalmology, Acibadem University, School of Medicine, Istanbul 34457, TurkeyDepartment of Ophthalmology, Faculty of Medicine, Bezmialem University, Istanbul 34093, TurkeyAIM: To investigate the effect of pomegranate juice (PJ) intake on overall oxidation status in retinas of diabetic rats. METHODS: Twenty-seven rats were divided into four groups as control (CO), diabetic (DM), control treated with PJ (CO-PJ), and diabetic treated with PJ (DM-PJ).The retina tissues were used to determine 8-hydroxy-2’-deoxyguanosine (8OHdG), malondialdehyde (MDA), reduced glutathione (GSH) levels, and the enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). RESULTS: The levels of 8OHdG and MDA were significantly increased in the retina of DM group compared to CO group (P=0.001, P<0.001 respectively). Both 8OHdG and MDA levels were decreased in PJ-DM group compared to DM group (P=0.004, P<0.001 respectively). The activities of antioxidant enzymes GSH, SOD, and GDH-Px were significantly decreased in the retina of DM group compared to CO group (P≤0.01). GSH and GSH-Px activities were higher in PJ-DM group compared with DM group (P=0.010, P=0.042, respectively) but SOD activity was not statistically different (P=0.938). CONCLUSION: PJ intake is found to be effective in decreasing oxidative end products, and in increasing the activities of antioxidant enzymes in diabetic retinas of rats, which suggests it may be effective against oxidative stress in diabetic retinas.http://www.ijo.cn/en_publish/2017/11/20171105.pdf1668antioxidant enzymesdiabetic retinopathyimmunohistochemical stainingoxidative stresspomegranate juice
collection DOAJ
language English
format Article
sources DOAJ
author Betul Tugcu
Senay Asik Nacaroglu
Asuman Gedikbasi
Mehmet Uhri
Nur Acar
Hakan Ozdemir
spellingShingle Betul Tugcu
Senay Asik Nacaroglu
Asuman Gedikbasi
Mehmet Uhri
Nur Acar
Hakan Ozdemir
Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats
International Journal of Ophthalmology
1668
antioxidant enzymes
diabetic retinopathy
immunohistochemical staining
oxidative stress
pomegranate juice
author_facet Betul Tugcu
Senay Asik Nacaroglu
Asuman Gedikbasi
Mehmet Uhri
Nur Acar
Hakan Ozdemir
author_sort Betul Tugcu
title Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats
title_short Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats
title_full Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats
title_fullStr Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats
title_full_unstemmed Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats
title_sort protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats
publisher Press of International Journal of Ophthalmology (IJO PRESS)
series International Journal of Ophthalmology
issn 2222-3959
2227-4898
publishDate 2017-11-01
description AIM: To investigate the effect of pomegranate juice (PJ) intake on overall oxidation status in retinas of diabetic rats. METHODS: Twenty-seven rats were divided into four groups as control (CO), diabetic (DM), control treated with PJ (CO-PJ), and diabetic treated with PJ (DM-PJ).The retina tissues were used to determine 8-hydroxy-2’-deoxyguanosine (8OHdG), malondialdehyde (MDA), reduced glutathione (GSH) levels, and the enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). RESULTS: The levels of 8OHdG and MDA were significantly increased in the retina of DM group compared to CO group (P=0.001, P<0.001 respectively). Both 8OHdG and MDA levels were decreased in PJ-DM group compared to DM group (P=0.004, P<0.001 respectively). The activities of antioxidant enzymes GSH, SOD, and GDH-Px were significantly decreased in the retina of DM group compared to CO group (P≤0.01). GSH and GSH-Px activities were higher in PJ-DM group compared with DM group (P=0.010, P=0.042, respectively) but SOD activity was not statistically different (P=0.938). CONCLUSION: PJ intake is found to be effective in decreasing oxidative end products, and in increasing the activities of antioxidant enzymes in diabetic retinas of rats, which suggests it may be effective against oxidative stress in diabetic retinas.
topic 1668
antioxidant enzymes
diabetic retinopathy
immunohistochemical staining
oxidative stress
pomegranate juice
url http://www.ijo.cn/en_publish/2017/11/20171105.pdf
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