MRGBP, a member of the NuA4 complex, inhibits DNA double‐strand break repair
The repair of DNA breaks takes place in the context of chromatin and thus involves the activity of chromatin remodelers. The nucleosome acetyltransferase of H4 (NuA4) remodeler complex enables DNA break repair by relaxing flanking chromatin. Here, we show that MRG domain binding protein (MRGBP), a m...
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Online Access: | https://doi.org/10.1002/2211-5463.13071 |
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doaj-a7b7abe3664d43cfb15a9c1840ad09112021-03-04T10:35:45ZengWileyFEBS Open Bio2211-54632021-03-0111362263210.1002/2211-5463.13071MRGBP, a member of the NuA4 complex, inhibits DNA double‐strand break repairSabrina Rivero0Guillermo Rodríguez‐Real1Inés Marín2Pablo Huertas3Department of Normal and Pathological Histology and Cytology University of Seville School of Medicine SpainCentro Andaluz de Biología Molecular y Medicina Regenerativa‐CABIMER Universidad de Sevilla‐CSIC‐Universidad Pablo de Olavide SpainCentro Andaluz de Biología Molecular y Medicina Regenerativa‐CABIMER Universidad de Sevilla‐CSIC‐Universidad Pablo de Olavide SpainCentro Andaluz de Biología Molecular y Medicina Regenerativa‐CABIMER Universidad de Sevilla‐CSIC‐Universidad Pablo de Olavide SpainThe repair of DNA breaks takes place in the context of chromatin and thus involves the activity of chromatin remodelers. The nucleosome acetyltransferase of H4 (NuA4) remodeler complex enables DNA break repair by relaxing flanking chromatin. Here, we show that MRG domain binding protein (MRGBP), a member of this complex, acts as a general inhibitor of DNA double‐strand break repair. Upon its downregulation, repair is generally increased. This is particularly evident for the stimulation of early events of homologous recombination. Thus, MRGBP has an opposing role to the main catalytic subunits of the NuA4 complex. Our data suggest that MRGBP acts by limiting the activity of this complex in DNA repair, specifically by narrowing the extent of DNA‐end resection.https://doi.org/10.1002/2211-5463.13071DNA repairDNA‐end resectionMRGBPrecombinationTIP60 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sabrina Rivero Guillermo Rodríguez‐Real Inés Marín Pablo Huertas |
spellingShingle |
Sabrina Rivero Guillermo Rodríguez‐Real Inés Marín Pablo Huertas MRGBP, a member of the NuA4 complex, inhibits DNA double‐strand break repair FEBS Open Bio DNA repair DNA‐end resection MRGBP recombination TIP60 |
author_facet |
Sabrina Rivero Guillermo Rodríguez‐Real Inés Marín Pablo Huertas |
author_sort |
Sabrina Rivero |
title |
MRGBP, a member of the NuA4 complex, inhibits DNA double‐strand break repair |
title_short |
MRGBP, a member of the NuA4 complex, inhibits DNA double‐strand break repair |
title_full |
MRGBP, a member of the NuA4 complex, inhibits DNA double‐strand break repair |
title_fullStr |
MRGBP, a member of the NuA4 complex, inhibits DNA double‐strand break repair |
title_full_unstemmed |
MRGBP, a member of the NuA4 complex, inhibits DNA double‐strand break repair |
title_sort |
mrgbp, a member of the nua4 complex, inhibits dna double‐strand break repair |
publisher |
Wiley |
series |
FEBS Open Bio |
issn |
2211-5463 |
publishDate |
2021-03-01 |
description |
The repair of DNA breaks takes place in the context of chromatin and thus involves the activity of chromatin remodelers. The nucleosome acetyltransferase of H4 (NuA4) remodeler complex enables DNA break repair by relaxing flanking chromatin. Here, we show that MRG domain binding protein (MRGBP), a member of this complex, acts as a general inhibitor of DNA double‐strand break repair. Upon its downregulation, repair is generally increased. This is particularly evident for the stimulation of early events of homologous recombination. Thus, MRGBP has an opposing role to the main catalytic subunits of the NuA4 complex. Our data suggest that MRGBP acts by limiting the activity of this complex in DNA repair, specifically by narrowing the extent of DNA‐end resection. |
topic |
DNA repair DNA‐end resection MRGBP recombination TIP60 |
url |
https://doi.org/10.1002/2211-5463.13071 |
work_keys_str_mv |
AT sabrinarivero mrgbpamemberofthenua4complexinhibitsdnadoublestrandbreakrepair AT guillermorodriguezreal mrgbpamemberofthenua4complexinhibitsdnadoublestrandbreakrepair AT inesmarin mrgbpamemberofthenua4complexinhibitsdnadoublestrandbreakrepair AT pablohuertas mrgbpamemberofthenua4complexinhibitsdnadoublestrandbreakrepair |
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