Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase for environmental welfare
As water contamination emerges as a serious threat to the environment, ventures for cleaner and sustainable solutions are continuously being developed. The present study investigates the ability of crude peroxidase extract from soybean seeds to degrade the anthraquinone dye Acid Violet 109. The infl...
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Society of Chemists and Technologists of Macedonia
2020-11-01
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doaj-a7c1662e441f4abe943330e2a1ab0c6f2021-02-24T18:21:02ZengSociety of Chemists and Technologists of MacedoniaMacedonian Journal of Chemistry and Chemical Engineering1857-55521857-56252020-11-0139219720610.20450/mjcce.2020.2150443Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase for environmental welfareMilica Milan Svetozarević0Nataša Šekuljica1Zorica Knežević-Jugović2Dušan Mijin3Innovation Center Faculty of Technology and Metallurgy, Karnegijeva 4, 11120 BelgradeInnovation Center of the Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 BelgradeUniversity of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 BelgradeUniversity of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 BelgradeAs water contamination emerges as a serious threat to the environment, ventures for cleaner and sustainable solutions are continuously being developed. The present study investigates the ability of crude peroxidase extract from soybean seeds to degrade the anthraquinone dye Acid Violet 109. The influence of the essential parameters pH, dye concentration, hydrogen peroxide dosage, and temperature were inspected. The enzyme had 81.9 % biodegradation at pH 4 in 30 min with 0.1 U peroxidase, 40 mg/l dye concentration, and 1 mM hydrogen peroxide. Considering that substrate concentration can cause reaction inhibition, a kinetic study was performed. Kinetic data fitting using bisubstrate kinetics with a substrate inhibition model revealed the high inhibitory effect of the dye, which was confirmed by the inhibition constant, 7.123·10–5 mM. Alongside the inhibition constant values, the Ping-Pong Bi-Bi model gave the maximum rates 15.788 and 14.321 mM/min for hydrogen peroxide and dye inhibition, respectively.https://mjcce.org.mk/index.php/MJCCE/article/view/2150soybean seed peroxidaseacid violet 109ping pong bi bidye treatment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Milica Milan Svetozarević Nataša Šekuljica Zorica Knežević-Jugović Dušan Mijin |
spellingShingle |
Milica Milan Svetozarević Nataša Šekuljica Zorica Knežević-Jugović Dušan Mijin Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase for environmental welfare Macedonian Journal of Chemistry and Chemical Engineering soybean seed peroxidase acid violet 109 ping pong bi bi dye treatment |
author_facet |
Milica Milan Svetozarević Nataša Šekuljica Zorica Knežević-Jugović Dušan Mijin |
author_sort |
Milica Milan Svetozarević |
title |
Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase for environmental welfare |
title_short |
Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase for environmental welfare |
title_full |
Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase for environmental welfare |
title_fullStr |
Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase for environmental welfare |
title_full_unstemmed |
Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase for environmental welfare |
title_sort |
optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase for environmental welfare |
publisher |
Society of Chemists and Technologists of Macedonia |
series |
Macedonian Journal of Chemistry and Chemical Engineering |
issn |
1857-5552 1857-5625 |
publishDate |
2020-11-01 |
description |
As water contamination emerges as a serious threat to the environment, ventures for cleaner and sustainable solutions are continuously being developed. The present study investigates the ability of crude peroxidase extract from soybean seeds to degrade the anthraquinone dye Acid Violet 109. The influence of the essential parameters pH, dye concentration, hydrogen peroxide dosage, and temperature were inspected. The enzyme had 81.9 % biodegradation at pH 4 in 30 min with 0.1 U peroxidase, 40 mg/l dye concentration, and 1 mM hydrogen peroxide. Considering that substrate concentration can cause reaction inhibition, a kinetic study was performed. Kinetic data fitting using bisubstrate kinetics with a substrate inhibition model revealed the high inhibitory effect of the dye, which was confirmed by the inhibition constant, 7.123·10–5 mM. Alongside the inhibition constant values, the Ping-Pong Bi-Bi model gave the maximum rates 15.788 and 14.321 mM/min for hydrogen peroxide and dye inhibition, respectively. |
topic |
soybean seed peroxidase acid violet 109 ping pong bi bi dye treatment |
url |
https://mjcce.org.mk/index.php/MJCCE/article/view/2150 |
work_keys_str_mv |
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