Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus
The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | srp |
Published: |
Faculty of Veterinary Medicine, Belgrade
2015-01-01
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Series: | Veterinarski Glasnik |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0350-2457/2015/0350-24571506337M.pdf |
Summary: | The objective of our work was to investigate some biological characteristics
of purified glycoprotein subunits of Newcastle disease virus strain
PHY-LMV.42 isolated from pigeons for the purpose of vaccine production.
PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive
passages in embryonated eggs and identified by the methods of Reverse
transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the
presence of HN and F antigene in the virus subunits samples was carried out
by hemagglutination inhibition method with referent immune sera. Biochemical
characterization of glycoprotein subunits was performed by SDS-PAGE method as
well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS).
Testing for the virus subunits immunogenicity was carried out in biological
experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing
an artificial infection with Hertz 33 strain of the virus. Low concentrations
of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i
58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The
subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune
response in all the vaccinated animals. Based on the obtained results it can
be concluded that low concentrations of purified virus subunits of PHY-LMV.42
strain can be used for preparing of effective vaccines. [Projekat
Ministarstva nauke Republike Srbije, br. TR 31008: Development and
application of molecular methods based on polymerase chain reaction (PCR) in
quick and direct identification of Newcastle disease virus strains and
investigation of immunogenicity of subunit vaccine prepared of their
antigens] |
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ISSN: | 0350-2457 2406-0771 |