DYNLRB1 is essential for dynein mediated transport and neuronal survival

• Main Authors: Marco Terenzio, Agostina Di Pizio, Ida Rishal, Letizia Marvaldi, Pierluigi Di Matteo, Riki Kawaguchi, Giovanni Coppola, Giampietro Schiavo, Elizabeth M.C. Fisher, Mike Fainzilber
• Format: Article
• Language: English
• Published: Elsevier 2020-07-01
• Series: Neurobiology of Disease
• Subjects: dynlrb1; Dynein complex; Axonal transport; Neuronal survival; Neurodegeneration
• Online Access: http://www.sciencedirect.com/science/article/pii/S0969996120300917

The cytoplasmic dynein motor complex transports essential signals and organelles from the cell periphery to the perinuclear region, hence is critical for the survival and function of highly polarized cells such as neurons. Dynein Light Chain Roadblock-Type 1 (DYNLRB1) is thought to be an accessory subunit required for specific cargos, but here we show that it is essential for general dynein-mediated transport and sensory neuron survival. Homozygous Dynlrb1 null mice are not viable and die during early embryonic development. Furthermore, heterozygous or adult knockdown animals display reduced neuronal growth, and selective depletion of Dynlrb1 in proprioceptive neurons compromises their survival. Conditional depletion of Dynlrb1 in sensory neurons causes deficits in several signaling pathways, including β-catenin subcellular localization, and a severe impairment in the axonal transport of both lysosomes and retrograde signaling endosomes. Hence, DYNLRB1 is an essential component of the dynein complex, and given dynein's critical functions in neuronal physiology, DYNLRB1 could have a prominent role in the etiology of human neurodegenerative diseases.