Establishment and characterization of two iPSC lines derived from healthy controls

Establishment and characterization of two iPSC lines derived from healthy controls

We have generated two iPSC lines from skin biopsies of two healthy individuals. Skin fibroblasts were derived and reprogrammed using a Sendai virus-based approach. The resulting iPSC lines have normal karyotype, express stemness markers and can generate endoderm, mesoderm and ectoderm in vitro. Thes...

Full description

Bibliographic Details
Main Authors: Achchhe Patel, Alejandro Garcia Diaz, Jennifer C. Moore, Dario Sirabella, Barbara Corneo
Format: Article
Language:English
Published: Elsevier 2020-08-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506120302270
id doaj-a7fd3b382c50420ab5c80efe1de5e1e1
record_format Article
spelling doaj-a7fd3b382c50420ab5c80efe1de5e1e12020-11-25T03:56:24ZengElsevierStem Cell Research1873-50612020-08-0147101926Establishment and characterization of two iPSC lines derived from healthy controlsAchchhe Patel0Alejandro Garcia Diaz1Jennifer C. Moore2Dario Sirabella3Barbara Corneo4Stem Cell Core Facility, Columbia University Irving Medical Center, NY, USAStem Cell Core Facility, Columbia University Irving Medical Center, NY, USARUCDR, Nelson Biological Laboratories, Piscataway, NJ, USAStem Cell Core Facility, Columbia University Irving Medical Center, NY, USAStem Cell Core Facility, Columbia University Irving Medical Center, NY, USA; Corresponding author.We have generated two iPSC lines from skin biopsies of two healthy individuals. Skin fibroblasts were derived and reprogrammed using a Sendai virus-based approach. The resulting iPSC lines have normal karyotype, express stemness markers and can generate endoderm, mesoderm and ectoderm in vitro. These iPSC lines can be used as healthy controls in differentiation paradigms as well as backbone for gene editing experiments.http://www.sciencedirect.com/science/article/pii/S1873506120302270
collection DOAJ
language English
format Article
sources DOAJ
author Achchhe Patel
Alejandro Garcia Diaz
Jennifer C. Moore
Dario Sirabella
Barbara Corneo
spellingShingle Achchhe Patel
Alejandro Garcia Diaz
Jennifer C. Moore
Dario Sirabella
Barbara Corneo
Establishment and characterization of two iPSC lines derived from healthy controls
Stem Cell Research
author_facet Achchhe Patel
Alejandro Garcia Diaz
Jennifer C. Moore
Dario Sirabella
Barbara Corneo
author_sort Achchhe Patel
title Establishment and characterization of two iPSC lines derived from healthy controls
title_short Establishment and characterization of two iPSC lines derived from healthy controls
title_full Establishment and characterization of two iPSC lines derived from healthy controls
title_fullStr Establishment and characterization of two iPSC lines derived from healthy controls
title_full_unstemmed Establishment and characterization of two iPSC lines derived from healthy controls
title_sort establishment and characterization of two ipsc lines derived from healthy controls
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-08-01
description We have generated two iPSC lines from skin biopsies of two healthy individuals. Skin fibroblasts were derived and reprogrammed using a Sendai virus-based approach. The resulting iPSC lines have normal karyotype, express stemness markers and can generate endoderm, mesoderm and ectoderm in vitro. These iPSC lines can be used as healthy controls in differentiation paradigms as well as backbone for gene editing experiments.
url http://www.sciencedirect.com/science/article/pii/S1873506120302270
work_keys_str_mv AT achchhepatel establishmentandcharacterizationoftwoipsclinesderivedfromhealthycontrols
AT alejandrogarciadiaz establishmentandcharacterizationoftwoipsclinesderivedfromhealthycontrols
AT jennifercmoore establishmentandcharacterizationoftwoipsclinesderivedfromhealthycontrols
AT dariosirabella establishmentandcharacterizationoftwoipsclinesderivedfromhealthycontrols
AT barbaracorneo establishmentandcharacterizationoftwoipsclinesderivedfromhealthycontrols
_version_ 1724465216991789056

Similar Items