Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene
Abstract Freshwater shrimp are a rich species group, with a long and problematic taxonomic history attributed to their wide distribution and similar morphological characteristics. Shrimp diversity and species identification are important cornerstones for fisheries management. However, identification...
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doaj-a7ff6336c8d245009be3589b23bf6d6a2021-08-02T18:48:04ZengWileyEcology and Evolution2045-77582021-08-011115105021051410.1002/ece3.7855Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial geneChuan‐jiang Zhou0Meng‐xia Feng1Yong‐tao Tang2Chang‐xing Yang3Xiao‐lin Meng4Guo‐xing Nie5College of Fisheries Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation Engineering Lab of Henan Province for Aquatic Animal Disease Control Henan Normal University Xinxiang ChinaCollege of Fisheries Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation Engineering Lab of Henan Province for Aquatic Animal Disease Control Henan Normal University Xinxiang ChinaCollege of Fisheries Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation Engineering Lab of Henan Province for Aquatic Animal Disease Control Henan Normal University Xinxiang ChinaCollege of Fisheries Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation Engineering Lab of Henan Province for Aquatic Animal Disease Control Henan Normal University Xinxiang ChinaCollege of Fisheries Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation Engineering Lab of Henan Province for Aquatic Animal Disease Control Henan Normal University Xinxiang ChinaCollege of Fisheries Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation Engineering Lab of Henan Province for Aquatic Animal Disease Control Henan Normal University Xinxiang ChinaAbstract Freshwater shrimp are a rich species group, with a long and problematic taxonomic history attributed to their wide distribution and similar morphological characteristics. Shrimp diversity and species identification are important cornerstones for fisheries management. However, identification based on morphological characteristics is a difficult task for a nonspecialist. Abundant freshwater shrimp species are distributed in the waters of Henan Province, but investigations of freshwater shrimp are limited in this region, especially concerning molecular features. Here, we combined morphology and DNA barcodes to reveal the species diversity of freshwater shrimp in Henan province. A total of 1,200 freshwater shrimp samples were collected from 46 sampling sites, and 222 samples were chosen for further microscopic examination and molecular delimitation. We used tree‐based methods (NJ, ML, and bPTP) and distance‐based methods (estimation of the paired genetic distances and ABGD) to delimit species. The results showed that there were nine morphospecies based on morphological characteristics; all could effectively be defined by molecular methods, among which bPTP and ABGD defined 13 and 8 MOTUs, respectively. The estimation of the paired genetic distances of K2P and the p‐distances had similar results. Mean K2P distances and p‐distances within species were both equal to 1.2%. The maximum intraspecific genetic distances of all species were less than 2%, with the exception of Palaemon modestus and M. maculatum. Various analyses have shown that P. modestus and M. maculatum have a large genetic differentiation, which may indicate the existence of cryptic species. By contrast, DNA barcoding could unambiguously discriminate 13 species and detect cryptic diversity. Our results demonstrate the high efficiency of DNA barcoding to delimit freshwater shrimp diversity and detect the presence of cryptic species.https://doi.org/10.1002/ece3.7855COIfreshwater shrimpsspecies delimitationspecies diversity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chuan‐jiang Zhou Meng‐xia Feng Yong‐tao Tang Chang‐xing Yang Xiao‐lin Meng Guo‐xing Nie |
spellingShingle |
Chuan‐jiang Zhou Meng‐xia Feng Yong‐tao Tang Chang‐xing Yang Xiao‐lin Meng Guo‐xing Nie Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene Ecology and Evolution COI freshwater shrimps species delimitation species diversity |
author_facet |
Chuan‐jiang Zhou Meng‐xia Feng Yong‐tao Tang Chang‐xing Yang Xiao‐lin Meng Guo‐xing Nie |
author_sort |
Chuan‐jiang Zhou |
title |
Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene |
title_short |
Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene |
title_full |
Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene |
title_fullStr |
Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene |
title_full_unstemmed |
Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene |
title_sort |
species diversity of freshwater shrimp in henan province, china, based on morphological characters and coi mitochondrial gene |
publisher |
Wiley |
series |
Ecology and Evolution |
issn |
2045-7758 |
publishDate |
2021-08-01 |
description |
Abstract Freshwater shrimp are a rich species group, with a long and problematic taxonomic history attributed to their wide distribution and similar morphological characteristics. Shrimp diversity and species identification are important cornerstones for fisheries management. However, identification based on morphological characteristics is a difficult task for a nonspecialist. Abundant freshwater shrimp species are distributed in the waters of Henan Province, but investigations of freshwater shrimp are limited in this region, especially concerning molecular features. Here, we combined morphology and DNA barcodes to reveal the species diversity of freshwater shrimp in Henan province. A total of 1,200 freshwater shrimp samples were collected from 46 sampling sites, and 222 samples were chosen for further microscopic examination and molecular delimitation. We used tree‐based methods (NJ, ML, and bPTP) and distance‐based methods (estimation of the paired genetic distances and ABGD) to delimit species. The results showed that there were nine morphospecies based on morphological characteristics; all could effectively be defined by molecular methods, among which bPTP and ABGD defined 13 and 8 MOTUs, respectively. The estimation of the paired genetic distances of K2P and the p‐distances had similar results. Mean K2P distances and p‐distances within species were both equal to 1.2%. The maximum intraspecific genetic distances of all species were less than 2%, with the exception of Palaemon modestus and M. maculatum. Various analyses have shown that P. modestus and M. maculatum have a large genetic differentiation, which may indicate the existence of cryptic species. By contrast, DNA barcoding could unambiguously discriminate 13 species and detect cryptic diversity. Our results demonstrate the high efficiency of DNA barcoding to delimit freshwater shrimp diversity and detect the presence of cryptic species. |
topic |
COI freshwater shrimps species delimitation species diversity |
url |
https://doi.org/10.1002/ece3.7855 |
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