Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia)
B chromosomes (Bs) are a very heterogeneous group of extra chromosomes. In various species Bs occur with different nucleotide sequences ranging from repetitive to protein coding. In yellow-necked field mice, Apodemus flavicollis Bs are small euchromatic chromosomes and untill now, only few...
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doaj-a83d6ae4b06e491cb45cb986e30e71712020-11-24T23:35:00ZengSerbian Genetics SocietyGenetika0534-00121820-60692015-01-0147131132110.2298/GENSR1501311R0534-00121501311RScreening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia)Rajičić Marija0Adnađević Tanja1Stamenković Gorana2Blagojević Jelena3Vujošević Mladen4Institute for Biological Research “Siniša Stanković”, Department of Genetic Research, BelgradeInstitute for Biological Research “Siniša Stanković”, Department of Genetic Research, BelgradeInstitute for Biological Research “Siniša Stanković”, Department of Genetic Research, BelgradeInstitute for Biological Research “Siniša Stanković”, Department of Genetic Research, BelgradeInstitute for Biological Research “Siniša Stanković”, Department of Genetic Research, BelgradeB chromosomes (Bs) are a very heterogeneous group of extra chromosomes. In various species Bs occur with different nucleotide sequences ranging from repetitive to protein coding. In yellow-necked field mice, Apodemus flavicollis Bs are small euchromatic chromosomes and untill now, only few molecular analyses have been conducted. In this study we examined A. flavicollis individuals with different number of Bs for presence of two genes, C-KIT and 18S rRNA. The C-KIT proto-oncogene was found on Bs in three Canidae species and one Cervidae species. This gene is a coding receptor critical for proliferation and cell differentiation of hematopoietic, melanoblast and primordial germ cells, and is highly conserved within mammals. While using semiquantitative PCR, we did not notice any difference in the C-KIT band intensity among animals with different number of Bs (0-3). The presence of only one copy of C- KIT gene was confirmed using real time-PCR on genomic DNA of A. flavicollis specimens with different number of Bs. rRNA genes in eukaryotes’ genome are organized like units of tandem repeated sequences. The units form distinct clusters on one to several chromosome pairs. rRNA genes were found on Bs in different species including two species of genus Apodemus. One particular sample with 2 Bs showed the number of 18S rRNA gene about three times that of the calibrator 0 B sample. This result can indicate the presence of 18S rRNA gene on Bs, but its confirmation requires the implementation of other methods. Still, we can neither confirm nor deny the existence of pseudogen of tested target genes, or lose of exon 1 of C-KIT protooncogen in Bs of A. flavicollis. Our findings are further discussed. [Projekat Ministarstva nauke Republike Srbije, br. 173003]http://www.doiserbia.nb.rs/img/doi/0534-0012/2015/0534-00121501311R.pdfApodemus flavicollisB chromosomesC-KIT18S rRNAsemiquantitative PCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rajičić Marija Adnađević Tanja Stamenković Gorana Blagojević Jelena Vujošević Mladen |
spellingShingle |
Rajičić Marija Adnađević Tanja Stamenković Gorana Blagojević Jelena Vujošević Mladen Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia) Genetika Apodemus flavicollis B chromosomes C-KIT 18S rRNA semiquantitative PCR |
author_facet |
Rajičić Marija Adnađević Tanja Stamenković Gorana Blagojević Jelena Vujošević Mladen |
author_sort |
Rajičić Marija |
title |
Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia) |
title_short |
Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia) |
title_full |
Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia) |
title_fullStr |
Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia) |
title_full_unstemmed |
Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia) |
title_sort |
screening of b chromosomes for presence of two genes in yellow-necked mice, apodemus flavicollis (mammalia, rodentia) |
publisher |
Serbian Genetics Society |
series |
Genetika |
issn |
0534-0012 1820-6069 |
publishDate |
2015-01-01 |
description |
B chromosomes (Bs) are a very heterogeneous group of extra chromosomes. In
various species Bs occur with different nucleotide sequences ranging from
repetitive to protein coding. In yellow-necked field mice, Apodemus
flavicollis Bs are small euchromatic chromosomes and untill now, only few
molecular analyses have been conducted. In this study we examined A.
flavicollis individuals with different number of Bs for presence of two
genes, C-KIT and 18S rRNA. The C-KIT proto-oncogene was found on Bs in three
Canidae species and one Cervidae species. This gene is a coding receptor
critical for proliferation and cell differentiation of hematopoietic,
melanoblast and primordial germ cells, and is highly conserved within
mammals. While using semiquantitative PCR, we did not notice any difference
in the C-KIT band intensity among animals with different number of Bs (0-3).
The presence of only one copy of C- KIT gene was confirmed using real
time-PCR on genomic DNA of A. flavicollis specimens with different number of
Bs. rRNA genes in eukaryotes’ genome are organized like units of tandem
repeated sequences. The units form distinct clusters on one to several
chromosome pairs. rRNA genes were found on Bs in different species including
two species of genus Apodemus. One particular sample with 2 Bs showed the
number of 18S rRNA gene about three times that of the calibrator 0 B sample.
This result can indicate the presence of 18S rRNA gene on Bs, but its
confirmation requires the implementation of other methods. Still, we can
neither confirm nor deny the existence of pseudogen of tested target genes,
or lose of exon 1 of C-KIT protooncogen in Bs of A. flavicollis. Our findings
are further discussed. [Projekat Ministarstva nauke Republike Srbije, br.
173003] |
topic |
Apodemus flavicollis B chromosomes C-KIT 18S rRNA semiquantitative PCR |
url |
http://www.doiserbia.nb.rs/img/doi/0534-0012/2015/0534-00121501311R.pdf |
work_keys_str_mv |
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