Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus)
Objective: To investigate the effects of hydroalcoholic extract of Ziziphus jujuba on the histopathological, tissue oxidative stress and inflammation plus to antioxidant pathways of colon tissue in rat with induced Ulcerative colitis. Materials and methods: Ulcerative colitis was induced in 80 rats...
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Format: | Article |
Language: | English |
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Thieme Revinter Publicações Ltda.
2016-10-01
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Series: | Journal of Coloproctology |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2237936316300326 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nader Tanideh Akram Jamshidzadeh Ali Ghanbari Saghesloo Farhad Rahmanifar Maral Mokhtari Omid Koohi-Hosseinabadi Mahmood Omidi Asma Najibi |
spellingShingle |
Nader Tanideh Akram Jamshidzadeh Ali Ghanbari Saghesloo Farhad Rahmanifar Maral Mokhtari Omid Koohi-Hosseinabadi Mahmood Omidi Asma Najibi Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus) Journal of Coloproctology |
author_facet |
Nader Tanideh Akram Jamshidzadeh Ali Ghanbari Saghesloo Farhad Rahmanifar Maral Mokhtari Omid Koohi-Hosseinabadi Mahmood Omidi Asma Najibi |
author_sort |
Nader Tanideh |
title |
Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus) |
title_short |
Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus) |
title_full |
Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus) |
title_fullStr |
Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus) |
title_full_unstemmed |
Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus) |
title_sort |
effects of hydroalcoholic extract of ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (rattus norvegicus) |
publisher |
Thieme Revinter Publicações Ltda. |
series |
Journal of Coloproctology |
issn |
2237-9363 |
publishDate |
2016-10-01 |
description |
Objective: To investigate the effects of hydroalcoholic extract of Ziziphus jujuba on the histopathological, tissue oxidative stress and inflammation plus to antioxidant pathways of colon tissue in rat with induced Ulcerative colitis. Materials and methods: Ulcerative colitis was induced in 80 rats those divided into 8 equal groups. Group 1 and 2 were negative controls receiving 1 mL/day of normal saline in enema and oral; group 3 and 4 as positive control 1 and 2 received 10 mg/kg of intra-colonic asacol and oral mesalazine; groups 5 and 6 received 20% and 40% of hydroalcoholic extract of Z. jujuba trans-rectally; group 7 and 8 received 1500 and 3000 mg/kg of hydroalcoholic extract of Z. jujuba orally, respectively. After 7 days, animals were evaluated for colon tissue histopathology, levels of malondialdehyde and IL-1β, and activities of superoxide dismutase, glutathione peroxidase and myeloperoxidase in colon tissue. Results: Hydroalcoholic extract of Z. jujuba in both forms of trans-rectal and oral administration especially in the higher doses could result into a more healing effect in damaged colonic tissue, more reduce glutathione peroxidase and IL-1β level. Also, these two doses (gel 40% and oral 3000 mg/kg) could more decrease the myeloperoxidase activity and stimulate superoxide dismutase and glutathione peroxidase activities. Also, gel 40% in transrectal administration was more potent than administration 3000 mg/kg in oral. Conclusion: The results of the present study indicated that Z. jujube may be considered as a treatment of choice for Ulcerative colitis especially in gel form and also in dose-dependent pattern. Resumo: Objetivo: Investigar os efeitos do extrato hidroalcoólico de Ziziphus jujuba no estresse oxidativo em tecido ao nível histopatológico e na inflamação, juntamente com as vias antioxidantes em tecido de cólon em ratos com colite ulcerativa induzida.Materiais e métodos: Induzimos colite ulcerativa em 80 ratos, divididos em 8 grupos iguais. Os grupos 1 e 2 eram controles negativos que receberam 1 mL/dia de salina normal em enema e por via oral; os grupos 3 e 4 eram controles positivos para 1 e 2 e receberam 10 mg/kg de asacol por via intracolônica e mesalazina oral; os grupos 5 e 6 receberam gel a 20% e 40% de extrato hidroalcoólico de Z. jujuba por via trans-retal; os grupos 7 e 8 receberam 1500 e 3000 mg/kg de extrato hidroalcoólico de Z. jujuba por via oral, respectivamente. Transcorridos 7 dias, os animais foram avaliados para histopatologia de tecido de cólon, níveis de malonildialdeído e IL-1β, e atividades de superóxido dismutase, glutátion peroxidase e mieloperoxidase no tecido colônico. Resultados: O uso do extrato hidroalcoólico de Z. jujuba, tanto na forma transretal como oral, e em especial nas doses mais altas, resultou em um efeito de cicatrização mais intensa no tecido colônico lesionado, e em maior redução nos níveis de glutátion peroxidase IL-1β. Além disso, essas duas doses (gel a 40% e 3000 mg/kg por via oral) diminuíram ainda mais a atividade de mieloperoxidase e estimularam as atividades de superóxido dismutase e glutátion peroxidase. Outro achado do estudo foi que o gel a 40% por administração trans-retal se mostrou mais potente do que a administração oral de 3000 mg/kg. Conclusão: Os resultados do presente estudo sugerem que Z. jujuba pode ser considerado como tratamento de escolha para colite ulcerativa, sobretudo em forma de gel e também em um padrão proporcional à dose administrada. Keywords: Ziziphus jujuba, Ulcerative colitis, Oxidative stress, Histopathology, Inflammation, Palavras-chave: Ziziphus jujuba, Colite ulcerativa, Estresse oxidativo, Histopatologia, Inflamação |
url |
http://www.sciencedirect.com/science/article/pii/S2237936316300326 |
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doaj-a84ca2aedc5e45f496997fcb69129ed22021-07-02T07:17:55ZengThieme Revinter Publicações Ltda.Journal of Coloproctology2237-93632016-10-01364189195Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus)Nader Tanideh0Akram Jamshidzadeh1Ali Ghanbari Saghesloo2Farhad Rahmanifar3Maral Mokhtari4Omid Koohi-Hosseinabadi5Mahmood Omidi6Asma Najibi7Shiraz University of Medical Sciences, Stem Cell and Transgenic Technology Research Center, Shiraz, Iran; Shiraz University of Medical Sciences, School of Medicine, Department of Pharmacology, Shiraz, IranShiraz University of Medical Sciences, School of Pharmacy, Department of Pharmacology Toxicology, Shiraz, IranShiraz University of Medical Sciences, School of Pharmacy, Department of Pharmacology Toxicology, Shiraz, Iran; Corresponding author.Shiraz University, School of Veterinary Medicine, Department of Anatomy, Shiraz, IranShiraz University of Medical Sciences, School of Medicine, Department of Pathology, Shiraz, IranShiraz University of Medical Sciences, Center of Comparative and Experimental Medicine, Shiraz, IranShiraz University of Medical Sciences, School of Pharmacy, Department of Pharmacology Toxicology, Shiraz, IranShiraz University of Medical Sciences, School of Pharmacy, Department of Pharmacology Toxicology, Shiraz, IranObjective: To investigate the effects of hydroalcoholic extract of Ziziphus jujuba on the histopathological, tissue oxidative stress and inflammation plus to antioxidant pathways of colon tissue in rat with induced Ulcerative colitis. Materials and methods: Ulcerative colitis was induced in 80 rats those divided into 8 equal groups. Group 1 and 2 were negative controls receiving 1 mL/day of normal saline in enema and oral; group 3 and 4 as positive control 1 and 2 received 10 mg/kg of intra-colonic asacol and oral mesalazine; groups 5 and 6 received 20% and 40% of hydroalcoholic extract of Z. jujuba trans-rectally; group 7 and 8 received 1500 and 3000 mg/kg of hydroalcoholic extract of Z. jujuba orally, respectively. After 7 days, animals were evaluated for colon tissue histopathology, levels of malondialdehyde and IL-1β, and activities of superoxide dismutase, glutathione peroxidase and myeloperoxidase in colon tissue. Results: Hydroalcoholic extract of Z. jujuba in both forms of trans-rectal and oral administration especially in the higher doses could result into a more healing effect in damaged colonic tissue, more reduce glutathione peroxidase and IL-1β level. Also, these two doses (gel 40% and oral 3000 mg/kg) could more decrease the myeloperoxidase activity and stimulate superoxide dismutase and glutathione peroxidase activities. Also, gel 40% in transrectal administration was more potent than administration 3000 mg/kg in oral. Conclusion: The results of the present study indicated that Z. jujube may be considered as a treatment of choice for Ulcerative colitis especially in gel form and also in dose-dependent pattern. Resumo: Objetivo: Investigar os efeitos do extrato hidroalcoólico de Ziziphus jujuba no estresse oxidativo em tecido ao nível histopatológico e na inflamação, juntamente com as vias antioxidantes em tecido de cólon em ratos com colite ulcerativa induzida.Materiais e métodos: Induzimos colite ulcerativa em 80 ratos, divididos em 8 grupos iguais. Os grupos 1 e 2 eram controles negativos que receberam 1 mL/dia de salina normal em enema e por via oral; os grupos 3 e 4 eram controles positivos para 1 e 2 e receberam 10 mg/kg de asacol por via intracolônica e mesalazina oral; os grupos 5 e 6 receberam gel a 20% e 40% de extrato hidroalcoólico de Z. jujuba por via trans-retal; os grupos 7 e 8 receberam 1500 e 3000 mg/kg de extrato hidroalcoólico de Z. jujuba por via oral, respectivamente. Transcorridos 7 dias, os animais foram avaliados para histopatologia de tecido de cólon, níveis de malonildialdeído e IL-1β, e atividades de superóxido dismutase, glutátion peroxidase e mieloperoxidase no tecido colônico. Resultados: O uso do extrato hidroalcoólico de Z. jujuba, tanto na forma transretal como oral, e em especial nas doses mais altas, resultou em um efeito de cicatrização mais intensa no tecido colônico lesionado, e em maior redução nos níveis de glutátion peroxidase IL-1β. Além disso, essas duas doses (gel a 40% e 3000 mg/kg por via oral) diminuíram ainda mais a atividade de mieloperoxidase e estimularam as atividades de superóxido dismutase e glutátion peroxidase. Outro achado do estudo foi que o gel a 40% por administração trans-retal se mostrou mais potente do que a administração oral de 3000 mg/kg. Conclusão: Os resultados do presente estudo sugerem que Z. jujuba pode ser considerado como tratamento de escolha para colite ulcerativa, sobretudo em forma de gel e também em um padrão proporcional à dose administrada. Keywords: Ziziphus jujuba, Ulcerative colitis, Oxidative stress, Histopathology, Inflammation, Palavras-chave: Ziziphus jujuba, Colite ulcerativa, Estresse oxidativo, Histopatologia, Inflamaçãohttp://www.sciencedirect.com/science/article/pii/S2237936316300326 |