Differential expression of miR-1297, miR-3191-5p, miR-4435, and miR-4465 in malignant and benign breast tumors
<em><strong>Objective(s):</strong></em> MicroRNAs (miRs) are a class of small non-coding RNAs which are associated with tumor growth and progression. In the present study, we assessed the expression of selected miRs in malignant, benign, and adjacent normal breast tissues. &l...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Mashhad University of Medical Sciences
2020-08-01
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Series: | Iranian Journal of Basic Medical Sciences |
Subjects: | |
Online Access: | http://ijbms.mums.ac.ir/article_15703_46b48a69cb124ab35800f339aca8319a.pdf |
Summary: | <em><strong>Objective(s):</strong></em> MicroRNAs (miRs) are a class of small non-coding RNAs which are associated with tumor growth and progression. In the present study, we assessed the expression of selected miRs in malignant, benign, and adjacent normal breast tissues. <br /><em><strong>Materials and Methods:</strong></em> The expression of miR-1297, miR-3191-5P, miR-4435, and miR-4465 were evaluated in malignant (n=50), benign (n=35), and adjacent normal breast tissues (n=20) using qRT-PCR. Receiver operating characteristic curves and the area under the ROC curve and AUC were generated for evaluating the diagnostic values of miRs. To evaluate diagnostic efficacy, miRs-based score was obtained using the logistic regression model. <br /><em><strong>Results:</strong></em> Among malignant tumors, the expression of miR-1297, miR-3191-5p, and miR-4435 was significantly lower (P=0.024, P<em><strong>Conclusion:</strong></em> The panel of these miRs are suggested as possible onco-miRs(miR-4465) or tumor suppressor-miRs (miR-3191-5P, miR-1297, miR-4435). Overall, our results indicated that these miRs could be introduced as diagnostic biomarkers in breast cancer patients. |
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ISSN: | 2008-3866 2008-3874 |