Differential expression of miR-1297, miR-3191-5p, miR-4435, and miR-4465 in malignant and benign breast tumors

• Main Authors: Abbas Mosapour, Fatemeh Soghra Karami Tehrani, Morteza Atri
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2020-08-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: biomarker; breast cancer; diagnosis; mir (microrna); oncomir
• Online Access: http://ijbms.mums.ac.ir/article_15703_46b48a69cb124ab35800f339aca8319a.pdf

<em><strong>Objective(s):</strong></em> MicroRNAs (miRs) are a class of small non-coding RNAs which are associated with tumor growth and progression. In the present study, we assessed the expression of selected miRs in malignant, benign, and adjacent normal breast tissues. <br /><em><strong>Materials and Methods:</strong></em> The expression of miR-1297, miR-3191-5P, miR-4435, and miR-4465 were evaluated in malignant (n=50), benign (n=35), and adjacent normal breast tissues (n=20) using qRT-PCR. Receiver operating characteristic curves and the area under the ROC curve and AUC were generated for evaluating the diagnostic values of miRs. To evaluate diagnostic efficacy, miRs-based score was obtained using the logistic regression model. <br /><em><strong>Results:</strong></em> Among malignant tumors, the expression of miR-1297, miR-3191-5p, and miR-4435 was significantly lower (P=0.024, P<em><strong>Conclusion:</strong></em> The panel of these miRs are suggested as possible onco-miRs(miR-4465) or tumor suppressor-miRs (miR-3191-5P, miR-1297, miR-4435). Overall, our results indicated that these miRs could be introduced as diagnostic biomarkers in breast cancer patients.