Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts
Plant species have traditionally been revered for their unparalleled pharmacognostic applications. We outline a non-iterative multi-parallel metabolomic-cum-bioassay-guided methodology toward the functional characterization of ethanol extracts from the Betulaceae family plants (<i>n</i>...
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doaj-a86b51d2ff334ec9a8345f21af7113e12020-11-25T02:48:02ZengMDPI AGMetabolites2218-19892019-09-019918610.3390/metabo9090186metabo9090186Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant ExtractsSunmin Lee0Dong-Gu Oh1Digar Singh2Hye Jin Lee3Ga Ryun Kim4Sarah Lee5Jong Seok Lee6Choong Hwan Lee7Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Biological Resources Utilization, National Institute of Biological Resources, Environmental Research Complex, Incheon 22755, KoreaDepartment of Biological Resources Utilization, National Institute of Biological Resources, Environmental Research Complex, Incheon 22755, KoreaDepartment of Biological Resources Utilization, National Institute of Biological Resources, Environmental Research Complex, Incheon 22755, KoreaDepartment of Biological Resources Utilization, National Institute of Biological Resources, Environmental Research Complex, Incheon 22755, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, KoreaPlant species have traditionally been revered for their unparalleled pharmacognostic applications. We outline a non-iterative multi-parallel metabolomic-cum-bioassay-guided methodology toward the functional characterization of ethanol extracts from the Betulaceae family plants (<i>n</i> = 10). We performed mass spectrometry (MS)-based multivariate analyses and bioassay-guided (ABTS antioxidant activity and cytoprotective effects against H<sub>2</sub>O<sub>2</sub>-induced cell damage) analyses of SPE fractions. A clearly distinct metabolomic pattern coupled with significantly higher bioactivities was observed for 40% methanol SPE eluate. Further, the 40% SPE eluate was subjected to preparative high-performance liquid chromatography (prep-HPLC) analysis, yielding 72 sub-fractions (1 min<sup>−1</sup>), with the highest antioxidant activities observed for the 15 min and 31 min sub-fractions. We simultaneously performed hyphenated-MS-based metabolite characterization of bioactive components for both the 40% methanol SPE fraction and its prep-HPLC sub-fraction (15 min and 31 min). Altogether, 19 candidate metabolites were mainly observed to contribute toward the observed bioactivities. In particular, ethyl gallate was mainly observed to affect the antioxidant activities of SPE and prep-HPLC fractions of <i>Alnus firma</i> extracts. We propose an integrated metabolomic-cum-bioassay-guided approach for the expeditious selection and characterization of discriminant metabolites with desired phenotypes or bioactivities.https://www.mdpi.com/2218-1989/9/9/186Betulaceae<i>Alnus firma</i>mass spectrometrybioassaysethyl gallate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sunmin Lee Dong-Gu Oh Digar Singh Hye Jin Lee Ga Ryun Kim Sarah Lee Jong Seok Lee Choong Hwan Lee |
spellingShingle |
Sunmin Lee Dong-Gu Oh Digar Singh Hye Jin Lee Ga Ryun Kim Sarah Lee Jong Seok Lee Choong Hwan Lee Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts Metabolites Betulaceae <i>Alnus firma</i> mass spectrometry bioassays ethyl gallate |
author_facet |
Sunmin Lee Dong-Gu Oh Digar Singh Hye Jin Lee Ga Ryun Kim Sarah Lee Jong Seok Lee Choong Hwan Lee |
author_sort |
Sunmin Lee |
title |
Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts |
title_short |
Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts |
title_full |
Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts |
title_fullStr |
Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts |
title_full_unstemmed |
Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts |
title_sort |
untargeted metabolomics toward systematic characterization of antioxidant compounds in betulaceae family plant extracts |
publisher |
MDPI AG |
series |
Metabolites |
issn |
2218-1989 |
publishDate |
2019-09-01 |
description |
Plant species have traditionally been revered for their unparalleled pharmacognostic applications. We outline a non-iterative multi-parallel metabolomic-cum-bioassay-guided methodology toward the functional characterization of ethanol extracts from the Betulaceae family plants (<i>n</i> = 10). We performed mass spectrometry (MS)-based multivariate analyses and bioassay-guided (ABTS antioxidant activity and cytoprotective effects against H<sub>2</sub>O<sub>2</sub>-induced cell damage) analyses of SPE fractions. A clearly distinct metabolomic pattern coupled with significantly higher bioactivities was observed for 40% methanol SPE eluate. Further, the 40% SPE eluate was subjected to preparative high-performance liquid chromatography (prep-HPLC) analysis, yielding 72 sub-fractions (1 min<sup>−1</sup>), with the highest antioxidant activities observed for the 15 min and 31 min sub-fractions. We simultaneously performed hyphenated-MS-based metabolite characterization of bioactive components for both the 40% methanol SPE fraction and its prep-HPLC sub-fraction (15 min and 31 min). Altogether, 19 candidate metabolites were mainly observed to contribute toward the observed bioactivities. In particular, ethyl gallate was mainly observed to affect the antioxidant activities of SPE and prep-HPLC fractions of <i>Alnus firma</i> extracts. We propose an integrated metabolomic-cum-bioassay-guided approach for the expeditious selection and characterization of discriminant metabolites with desired phenotypes or bioactivities. |
topic |
Betulaceae <i>Alnus firma</i> mass spectrometry bioassays ethyl gallate |
url |
https://www.mdpi.com/2218-1989/9/9/186 |
work_keys_str_mv |
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