Identification of Enterocytozoon bieneusi in goats and cattle in Thailand
Abstract Background Enterocytozoon bieneusi has been increasingly reported to infect domestic animals and humans, with human infections primarily reported as zoonotic in origin. The aim of the present study was to determine the presence and genotype of E. bieneusi in humans and domestic animals in c...
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doaj-a894ff8597b54242a4c9605baa6820ab2020-11-25T03:49:27ZengBMCBMC Veterinary Research1746-61482019-08-011511710.1186/s12917-019-2054-yIdentification of Enterocytozoon bieneusi in goats and cattle in ThailandRuenruetai Udonsom0Rapeepun Prasertbun1Aongart Mahittikorn2Rachatawan Chiabchalard3Chantira Sutthikornchai4Attakorn Palasuwan5Supaluk Popruk6Department of Protozoology, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol UniversityOxidation in Red Cell Disorders and Health Task Force, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn UniversityDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol UniversityAbstract Background Enterocytozoon bieneusi has been increasingly reported to infect domestic animals and humans, with human infections primarily reported as zoonotic in origin. The aim of the present study was to determine the presence and genotype of E. bieneusi in humans and domestic animals in central Thailand by testing stool samples of 200 apparently healthy humans, 73 goats, 60 cattle and 65 pigs using nested-PCR/ sequence analysis based on the ITS region of SSU rRNA genes. Results E. bieneusi tested positive in 2 (1%) of the 200 stool samples collected from humans and 56 (28.3%) of the 198 stool samples collected from domestic animals. The highest prevalence of E. bieneusi was observed in pigs (39/65, 60%), followed by goats (14/73, 19.2%) and cattle (3/60, 5%). Seven novel E. bieneusi genotypes were identified, which were named GoatAYE1–4 and PigAYE1–3 and clustered in either zoonotic Group 1 or Group 2. Moreover, eleven previously described E. bieneusi genotypes were also identified (O, D, H, SX1, CHC8, CHG3, CS-10, SHZC1, LW1, WildBoar5, and EbpC). All novel genotypes exhibited zoonotic potential from a phylogenetic analysis of ITS region. Conclusion Our data showed that the prevalence of E. bieneusi is low in apparently healthy individuals and higher in pigs than cattle and goats. This study provides baseline data useful for controlling and preventing E. bieneusi infection in farm communities, where pigs and goats appear to be the major reservoir of E. bieneusi. The results of our study support the view that E. bieneusi is a zoonotic pathogen that should be considered a potential public health threat.http://link.springer.com/article/10.1186/s12917-019-2054-yEnterocytozoon bieneusiGoatsCattlePigsZoonotic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ruenruetai Udonsom Rapeepun Prasertbun Aongart Mahittikorn Rachatawan Chiabchalard Chantira Sutthikornchai Attakorn Palasuwan Supaluk Popruk |
spellingShingle |
Ruenruetai Udonsom Rapeepun Prasertbun Aongart Mahittikorn Rachatawan Chiabchalard Chantira Sutthikornchai Attakorn Palasuwan Supaluk Popruk Identification of Enterocytozoon bieneusi in goats and cattle in Thailand BMC Veterinary Research Enterocytozoon bieneusi Goats Cattle Pigs Zoonotic |
author_facet |
Ruenruetai Udonsom Rapeepun Prasertbun Aongart Mahittikorn Rachatawan Chiabchalard Chantira Sutthikornchai Attakorn Palasuwan Supaluk Popruk |
author_sort |
Ruenruetai Udonsom |
title |
Identification of Enterocytozoon bieneusi in goats and cattle in Thailand |
title_short |
Identification of Enterocytozoon bieneusi in goats and cattle in Thailand |
title_full |
Identification of Enterocytozoon bieneusi in goats and cattle in Thailand |
title_fullStr |
Identification of Enterocytozoon bieneusi in goats and cattle in Thailand |
title_full_unstemmed |
Identification of Enterocytozoon bieneusi in goats and cattle in Thailand |
title_sort |
identification of enterocytozoon bieneusi in goats and cattle in thailand |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2019-08-01 |
description |
Abstract Background Enterocytozoon bieneusi has been increasingly reported to infect domestic animals and humans, with human infections primarily reported as zoonotic in origin. The aim of the present study was to determine the presence and genotype of E. bieneusi in humans and domestic animals in central Thailand by testing stool samples of 200 apparently healthy humans, 73 goats, 60 cattle and 65 pigs using nested-PCR/ sequence analysis based on the ITS region of SSU rRNA genes. Results E. bieneusi tested positive in 2 (1%) of the 200 stool samples collected from humans and 56 (28.3%) of the 198 stool samples collected from domestic animals. The highest prevalence of E. bieneusi was observed in pigs (39/65, 60%), followed by goats (14/73, 19.2%) and cattle (3/60, 5%). Seven novel E. bieneusi genotypes were identified, which were named GoatAYE1–4 and PigAYE1–3 and clustered in either zoonotic Group 1 or Group 2. Moreover, eleven previously described E. bieneusi genotypes were also identified (O, D, H, SX1, CHC8, CHG3, CS-10, SHZC1, LW1, WildBoar5, and EbpC). All novel genotypes exhibited zoonotic potential from a phylogenetic analysis of ITS region. Conclusion Our data showed that the prevalence of E. bieneusi is low in apparently healthy individuals and higher in pigs than cattle and goats. This study provides baseline data useful for controlling and preventing E. bieneusi infection in farm communities, where pigs and goats appear to be the major reservoir of E. bieneusi. The results of our study support the view that E. bieneusi is a zoonotic pathogen that should be considered a potential public health threat. |
topic |
Enterocytozoon bieneusi Goats Cattle Pigs Zoonotic |
url |
http://link.springer.com/article/10.1186/s12917-019-2054-y |
work_keys_str_mv |
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