Establishment of human embryonic stem cell line Amicqui-2 using poor-quality embryos from Mexican population

• Main Authors: Daniela Ávila-González, Omar Martínez-Alarcón, Guadalupe García-López, Néstor Emmanuel Díaz-Martínez, Guadalupe Razo-Aguilera, María Yolotzin Valdespino-Vázquez, Elsa Romelia Moreno-Verduzco, Eva Vega-Hernández, Juan Carlos Regalado-Hernández, Julio Francisco De la Jara-Díaz, Anayansi Molina-Hernández, Héctor Flores-Herrera, Wendy Portillo, Néstor Fabián Díaz
• Format: Article
• Language: English
• Published: Elsevier 2019-01-01
• Series: Stem Cell Research
• Online Access: http://www.sciencedirect.com/science/article/pii/S187350611830299X

Although investigation with human embryonic stem cells (HESC) is not decreasing, the derivation of new lines has been diminished. The preeminence of only a few HESC lines in research is accompanied by lack of universal applicability of results as well as by genetic under-representation. We previously reported the derivation of one line with male karyotype from Mexican population. Here, we derived one HESC line (Amicqui-2) with female karyotype from poor-quality embryos. These line comply the pluripotent requirements (normal karyotype, detection of pluripotency-associated markers, mycoplasma test and teratoma formation) and could be a valuable model for studying diseases specific to under-represented population.