Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis
Ophiocordyceps xuefengensis, a recently described species of Ophiocordyceps that is associated with the larvae of Phassus nodus (Hepialidae) in the living root or trunk of the medicinal plant Clerodendrum cyrtophyllum, is the largest known Cordyceps species and is recognized as a desirable alternati...
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doaj-a8a4162d7f864670bc0243719a17811b2020-11-25T00:52:59ZengMDPI AGMolecules1420-30492017-09-01229153010.3390/molecules22091530molecules22091530Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensisJuan Zou0Ling Wu1Zheng-Mi He2Ping Zhang3Zuo-Hong Chen4College of Life Science, Hunan Normal University, Changsha 410081, ChinaCollege of Life Science, Hunan Normal University, Changsha 410081, ChinaCollege of Life Science, Hunan Normal University, Changsha 410081, ChinaCollege of Life Science, Hunan Normal University, Changsha 410081, ChinaCollege of Life Science, Hunan Normal University, Changsha 410081, ChinaOphiocordyceps xuefengensis, a recently described species of Ophiocordyceps that is associated with the larvae of Phassus nodus (Hepialidae) in the living root or trunk of the medicinal plant Clerodendrum cyrtophyllum, is the largest known Cordyceps species and is recognized as a desirable alternative for natural Ophiocordyceps sinensis. This study investigated the main nucleosides and nucleobases in natural and cultured Ophiocordyceps xuefengensis. The contents of the nucleosides and nucleobases in the natural and cultured samples were determined by reverse phase HPLC. The highest concentration of adenosine was found in the natural fruit body and the cultured stroma, with almost no adenosine in the cadaver of Phassus nodus. The contents of adenine, guanosine, uridine and uracil in the cultured mycelium were significantly higher than those in the natural sample. Inosine was only detected in the natural samples. Thymidine and 2-deoxyadenosine were only found in the cadaver of Phassus nodus. Cordycepin was not detected in the five samples examined. These results suggested that the cultured mycelium and cultured stroma of Ophiocordyceps xuefengensis might be a promising substitute for natural O. xuefengensis.https://www.mdpi.com/1420-3049/22/9/1530Ophiocordyceps xuefengensisPhassus nodusstromanucleosides |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan Zou Ling Wu Zheng-Mi He Ping Zhang Zuo-Hong Chen |
spellingShingle |
Juan Zou Ling Wu Zheng-Mi He Ping Zhang Zuo-Hong Chen Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis Molecules Ophiocordyceps xuefengensis Phassus nodus stroma nucleosides |
author_facet |
Juan Zou Ling Wu Zheng-Mi He Ping Zhang Zuo-Hong Chen |
author_sort |
Juan Zou |
title |
Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis |
title_short |
Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis |
title_full |
Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis |
title_fullStr |
Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis |
title_full_unstemmed |
Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis |
title_sort |
determination of the main nucleosides and nucleobases in natural and cultured ophiocordyceps xuefengensis |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2017-09-01 |
description |
Ophiocordyceps xuefengensis, a recently described species of Ophiocordyceps that is associated with the larvae of Phassus nodus (Hepialidae) in the living root or trunk of the medicinal plant Clerodendrum cyrtophyllum, is the largest known Cordyceps species and is recognized as a desirable alternative for natural Ophiocordyceps sinensis. This study investigated the main nucleosides and nucleobases in natural and cultured Ophiocordyceps xuefengensis. The contents of the nucleosides and nucleobases in the natural and cultured samples were determined by reverse phase HPLC. The highest concentration of adenosine was found in the natural fruit body and the cultured stroma, with almost no adenosine in the cadaver of Phassus nodus. The contents of adenine, guanosine, uridine and uracil in the cultured mycelium were significantly higher than those in the natural sample. Inosine was only detected in the natural samples. Thymidine and 2-deoxyadenosine were only found in the cadaver of Phassus nodus. Cordycepin was not detected in the five samples examined. These results suggested that the cultured mycelium and cultured stroma of Ophiocordyceps xuefengensis might be a promising substitute for natural O. xuefengensis. |
topic |
Ophiocordyceps xuefengensis Phassus nodus stroma nucleosides |
url |
https://www.mdpi.com/1420-3049/22/9/1530 |
work_keys_str_mv |
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