Apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cells
Objectives: Silver nanoparticles (AgNPs) are one of the most vital and fascinating nanomaterials used in biomedical applications. Moreover, natural products from medicinal plants provide unlimited opportunities for drug development owing to the unmatched availability of a biochemical diversity. In t...
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doaj-a8a8cbee277a4997a84541dc505307d42020-11-25T03:33:18ZengElsevierJournal of King Saud University: Science1018-36472020-06-0132424802488Apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cellsAhmed Ali Al-kawmani0Khalid Mashai Alanazi1Mohammad Abul Farah2M. Ajmal Ali3Waleed Ali Qaid Hailan4Fahad M.A. Al-Hemaid5Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Corresponding author.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaObjectives: Silver nanoparticles (AgNPs) are one of the most vital and fascinating nanomaterials used in biomedical applications. Moreover, natural products from medicinal plants provide unlimited opportunities for drug development owing to the unmatched availability of a biochemical diversity. In this study, we biosynthesized AgNPs from the leaf extract of Ochradenus arabicus using silver nitrate and evaluated the cytotoxicity and apoptosis inducing ability of the O. arabicus AgNPs (OA-synthesized AgNPs) against the MCF-7 human breast cancer cells. Methods: The OA-synthesized AgNPs were characterized by X-ray diffraction, UV–visible spectroscopy, and transmission electron microscopy. Results: The OA-synthesized AgNPs were highly toxic to the MCF-7 cells as the cell proliferation was significantly decreased post-treatment. The intracellular level of reactive oxygen species (ROS) was significantly increased (p < 0.05). Moreover, a dose-dependent increase in the percentage of apoptosis and autophagy was also evident. Conclusion: The findings of this study confirm the ability of OA-synthesized AgNPs to stimulate apoptosis and suppress cell growth and proliferation.http://www.sciencedirect.com/science/article/pii/S101836472030149XOchradenus arabicusSilver nanoparticlesApoptosisAutophagyReactive oxygen speciesCytotoxicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed Ali Al-kawmani Khalid Mashai Alanazi Mohammad Abul Farah M. Ajmal Ali Waleed Ali Qaid Hailan Fahad M.A. Al-Hemaid |
spellingShingle |
Ahmed Ali Al-kawmani Khalid Mashai Alanazi Mohammad Abul Farah M. Ajmal Ali Waleed Ali Qaid Hailan Fahad M.A. Al-Hemaid Apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cells Journal of King Saud University: Science Ochradenus arabicus Silver nanoparticles Apoptosis Autophagy Reactive oxygen species Cytotoxicity |
author_facet |
Ahmed Ali Al-kawmani Khalid Mashai Alanazi Mohammad Abul Farah M. Ajmal Ali Waleed Ali Qaid Hailan Fahad M.A. Al-Hemaid |
author_sort |
Ahmed Ali Al-kawmani |
title |
Apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cells |
title_short |
Apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cells |
title_full |
Apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cells |
title_fullStr |
Apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cells |
title_full_unstemmed |
Apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cells |
title_sort |
apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cells |
publisher |
Elsevier |
series |
Journal of King Saud University: Science |
issn |
1018-3647 |
publishDate |
2020-06-01 |
description |
Objectives: Silver nanoparticles (AgNPs) are one of the most vital and fascinating nanomaterials used in biomedical applications. Moreover, natural products from medicinal plants provide unlimited opportunities for drug development owing to the unmatched availability of a biochemical diversity. In this study, we biosynthesized AgNPs from the leaf extract of Ochradenus arabicus using silver nitrate and evaluated the cytotoxicity and apoptosis inducing ability of the O. arabicus AgNPs (OA-synthesized AgNPs) against the MCF-7 human breast cancer cells. Methods: The OA-synthesized AgNPs were characterized by X-ray diffraction, UV–visible spectroscopy, and transmission electron microscopy. Results: The OA-synthesized AgNPs were highly toxic to the MCF-7 cells as the cell proliferation was significantly decreased post-treatment. The intracellular level of reactive oxygen species (ROS) was significantly increased (p < 0.05). Moreover, a dose-dependent increase in the percentage of apoptosis and autophagy was also evident. Conclusion: The findings of this study confirm the ability of OA-synthesized AgNPs to stimulate apoptosis and suppress cell growth and proliferation. |
topic |
Ochradenus arabicus Silver nanoparticles Apoptosis Autophagy Reactive oxygen species Cytotoxicity |
url |
http://www.sciencedirect.com/science/article/pii/S101836472030149X |
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