Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic <it>Escherichia coli </it>strains with different uncommon intimin types
<p>Abstract</p> <p>Background</p> <p>Enteropathogenic <it>Escherichia coli </it>(EPEC) produce attaching/effacing (A/E) lesions on eukaryotic cells mediated by the outer membrane adhesin intimin. EPEC are sub-grouped into typical (tEPEC) and atypical (aEPEC)...
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doaj-a8afd5ea270b47c7ba0148927c05e6ca2020-11-25T01:13:45ZengBMCBMC Microbiology1471-21802009-07-019114610.1186/1471-2180-9-146Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic <it>Escherichia coli </it>strains with different uncommon intimin typesBlanco Jesús EDahbi GhizlaneCarneiro Sylvia MSchmidt M AlexanderGreune LiloBlanco MiguelHernandes Rodrigo TYamamoto DeniseMora AzucenaBlanco JorgeGomes Tânia AT<p>Abstract</p> <p>Background</p> <p>Enteropathogenic <it>Escherichia coli </it>(EPEC) produce attaching/effacing (A/E) lesions on eukaryotic cells mediated by the outer membrane adhesin intimin. EPEC are sub-grouped into typical (tEPEC) and atypical (aEPEC). We have recently demonstrated that aEPEC strain 1551-2 (serotype O non-typable, non-motile) invades HeLa cells by a process dependent on the expression of intimin sub-type omicron. In this study, we evaluated whether aEPEC strains expressing other intimin sub-types are also invasive using the quantitative gentamicin protection assay. We also evaluated whether aEPEC invade differentiated intestinal T84 cells.</p> <p>Results</p> <p>Five of six strains invaded HeLa and T84 cells in a range of 13.3%–20.9% and 5.8%–17.8%, respectively, of the total cell-associated bacteria. The strains studied were significantly more invasive than prototype tEPEC strain E2348/69 (1.4% and 0.5% in HeLa and T84 cells, respectively). Invasiveness was confirmed by transmission electron microscopy. We also showed that invasion of HeLa cells by aEPEC 1551-2 depended on actin filaments, but not on microtubules. In addition, disruption of tight junctions enhanced its invasion efficiency in T84 cells, suggesting preferential invasion via a non-differentiated surface.</p> <p>Conclusion</p> <p>Some aEPEC strains may invade intestinal cells <it>in vitro </it>with varying efficiencies and independently of the intimin sub-type.</p> http://www.biomedcentral.com/1471-2180/9/146 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Blanco Jesús E Dahbi Ghizlane Carneiro Sylvia M Schmidt M Alexander Greune Lilo Blanco Miguel Hernandes Rodrigo T Yamamoto Denise Mora Azucena Blanco Jorge Gomes Tânia AT |
spellingShingle |
Blanco Jesús E Dahbi Ghizlane Carneiro Sylvia M Schmidt M Alexander Greune Lilo Blanco Miguel Hernandes Rodrigo T Yamamoto Denise Mora Azucena Blanco Jorge Gomes Tânia AT Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic <it>Escherichia coli </it>strains with different uncommon intimin types BMC Microbiology |
author_facet |
Blanco Jesús E Dahbi Ghizlane Carneiro Sylvia M Schmidt M Alexander Greune Lilo Blanco Miguel Hernandes Rodrigo T Yamamoto Denise Mora Azucena Blanco Jorge Gomes Tânia AT |
author_sort |
Blanco Jesús E |
title |
Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic <it>Escherichia coli </it>strains with different uncommon intimin types |
title_short |
Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic <it>Escherichia coli </it>strains with different uncommon intimin types |
title_full |
Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic <it>Escherichia coli </it>strains with different uncommon intimin types |
title_fullStr |
Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic <it>Escherichia coli </it>strains with different uncommon intimin types |
title_full_unstemmed |
Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic <it>Escherichia coli </it>strains with different uncommon intimin types |
title_sort |
invasiveness as a putative additional virulence mechanism of some atypical enteropathogenic <it>escherichia coli </it>strains with different uncommon intimin types |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2009-07-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Enteropathogenic <it>Escherichia coli </it>(EPEC) produce attaching/effacing (A/E) lesions on eukaryotic cells mediated by the outer membrane adhesin intimin. EPEC are sub-grouped into typical (tEPEC) and atypical (aEPEC). We have recently demonstrated that aEPEC strain 1551-2 (serotype O non-typable, non-motile) invades HeLa cells by a process dependent on the expression of intimin sub-type omicron. In this study, we evaluated whether aEPEC strains expressing other intimin sub-types are also invasive using the quantitative gentamicin protection assay. We also evaluated whether aEPEC invade differentiated intestinal T84 cells.</p> <p>Results</p> <p>Five of six strains invaded HeLa and T84 cells in a range of 13.3%–20.9% and 5.8%–17.8%, respectively, of the total cell-associated bacteria. The strains studied were significantly more invasive than prototype tEPEC strain E2348/69 (1.4% and 0.5% in HeLa and T84 cells, respectively). Invasiveness was confirmed by transmission electron microscopy. We also showed that invasion of HeLa cells by aEPEC 1551-2 depended on actin filaments, but not on microtubules. In addition, disruption of tight junctions enhanced its invasion efficiency in T84 cells, suggesting preferential invasion via a non-differentiated surface.</p> <p>Conclusion</p> <p>Some aEPEC strains may invade intestinal cells <it>in vitro </it>with varying efficiencies and independently of the intimin sub-type.</p> |
url |
http://www.biomedcentral.com/1471-2180/9/146 |
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