Callus formation and plant regeneration from protoplasts of sunflower calli and hypocotyls
Sunflower (cv. Girapac SH222) protoplasts were obtained from 4-7 day-old hypocotyls and cotyledons and from two-month old calli. Higher yields of protoplasts were achieved with medium El (KCl 25g dm-3, CaCl2 2g dm-3, MES 0.7 g• dm-3, pH 5.5) and the combination of Driselase Fluka 0.2%, Macerozyme On...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Polish Botanical Society
2014-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/811 |