Recent Trends in Plant Protein Complex Analysis in a Developmental Context
Because virtually all proteins interact with other proteins, studying protein–protein interactions (PPIs) is fundamental in understanding protein function. This is especially true when studying specific developmental processes, in which proteins often make developmental stage- or tissue specific int...
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doaj-a907680e4c22402d8372c7a6b2068da52020-11-24T22:53:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-05-01910.3389/fpls.2018.00640358535Recent Trends in Plant Protein Complex Analysis in a Developmental ContextMichiel Bontinck0Michiel Bontinck1Jelle Van Leene2Jelle Van Leene3Astrid Gadeyne4Astrid Gadeyne5Bert De Rybel6Bert De Rybel7Dominique Eeckhout8Dominique Eeckhout9Hilde Nelissen10Hilde Nelissen11Geert De Jaeger12Geert De Jaeger13Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology, VIB-UGent Center for Plant Systems Biology, Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology, VIB-UGent Center for Plant Systems Biology, Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology, VIB-UGent Center for Plant Systems Biology, Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology, VIB-UGent Center for Plant Systems Biology, Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology, VIB-UGent Center for Plant Systems Biology, Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology, VIB-UGent Center for Plant Systems Biology, Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology, VIB-UGent Center for Plant Systems Biology, Ghent, BelgiumBecause virtually all proteins interact with other proteins, studying protein–protein interactions (PPIs) is fundamental in understanding protein function. This is especially true when studying specific developmental processes, in which proteins often make developmental stage- or tissue specific interactions. However, studying these specific PPIs in planta can be challenging. One of the most widely adopted methods to study PPIs in planta is affinity purification coupled to mass spectrometry (AP/MS). Recent developments in the field of mass spectrometry have boosted applications of AP/MS in a developmental context. This review covers two main advancements in the field of affinity purification to study plant developmental processes: increasing the developmental resolution of the harvested tissues and moving from affinity purification to affinity enrichment. Furthermore, we discuss some new affinity purification approaches that have recently emerged and could have a profound impact on the future of protein interactome analysis in plants.http://journal.frontiersin.org/article/10.3389/fpls.2018.00640/fullplant developmentaffinity enrichmenttandem affinity purificationArabidopsis thalianainteractomicsproximity-dependent labeling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michiel Bontinck Michiel Bontinck Jelle Van Leene Jelle Van Leene Astrid Gadeyne Astrid Gadeyne Bert De Rybel Bert De Rybel Dominique Eeckhout Dominique Eeckhout Hilde Nelissen Hilde Nelissen Geert De Jaeger Geert De Jaeger |
spellingShingle |
Michiel Bontinck Michiel Bontinck Jelle Van Leene Jelle Van Leene Astrid Gadeyne Astrid Gadeyne Bert De Rybel Bert De Rybel Dominique Eeckhout Dominique Eeckhout Hilde Nelissen Hilde Nelissen Geert De Jaeger Geert De Jaeger Recent Trends in Plant Protein Complex Analysis in a Developmental Context Frontiers in Plant Science plant development affinity enrichment tandem affinity purification Arabidopsis thaliana interactomics proximity-dependent labeling |
author_facet |
Michiel Bontinck Michiel Bontinck Jelle Van Leene Jelle Van Leene Astrid Gadeyne Astrid Gadeyne Bert De Rybel Bert De Rybel Dominique Eeckhout Dominique Eeckhout Hilde Nelissen Hilde Nelissen Geert De Jaeger Geert De Jaeger |
author_sort |
Michiel Bontinck |
title |
Recent Trends in Plant Protein Complex Analysis in a Developmental Context |
title_short |
Recent Trends in Plant Protein Complex Analysis in a Developmental Context |
title_full |
Recent Trends in Plant Protein Complex Analysis in a Developmental Context |
title_fullStr |
Recent Trends in Plant Protein Complex Analysis in a Developmental Context |
title_full_unstemmed |
Recent Trends in Plant Protein Complex Analysis in a Developmental Context |
title_sort |
recent trends in plant protein complex analysis in a developmental context |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2018-05-01 |
description |
Because virtually all proteins interact with other proteins, studying protein–protein interactions (PPIs) is fundamental in understanding protein function. This is especially true when studying specific developmental processes, in which proteins often make developmental stage- or tissue specific interactions. However, studying these specific PPIs in planta can be challenging. One of the most widely adopted methods to study PPIs in planta is affinity purification coupled to mass spectrometry (AP/MS). Recent developments in the field of mass spectrometry have boosted applications of AP/MS in a developmental context. This review covers two main advancements in the field of affinity purification to study plant developmental processes: increasing the developmental resolution of the harvested tissues and moving from affinity purification to affinity enrichment. Furthermore, we discuss some new affinity purification approaches that have recently emerged and could have a profound impact on the future of protein interactome analysis in plants. |
topic |
plant development affinity enrichment tandem affinity purification Arabidopsis thaliana interactomics proximity-dependent labeling |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2018.00640/full |
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