A Novel Mouse Model of Penetrating Brain Injury
Penetrating traumatic brain injury (pTBI) has been difficult to model in small laboratory animals, such as rats or mice. Previously, we have established a non-fatal, rat model for penetrating brain injury using a modified air-rifle that accelerates a pellet, which hits a small probe that then penetr...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2014-10-01
|
Series: | Frontiers in Neurology |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fneur.2014.00209/full |
id |
doaj-a918863a4f4d4b478408c7b1ef1a1d4c |
---|---|
record_format |
Article |
spelling |
doaj-a918863a4f4d4b478408c7b1ef1a1d4c2020-11-24T22:06:46ZengFrontiers Media S.A.Frontiers in Neurology1664-22952014-10-01510.3389/fneur.2014.00209108883A Novel Mouse Model of Penetrating Brain InjuryIbolja eCernak0Ian D. Wing1Johan eDavidsson2Stefan ePlantman3University of AlbertaJohns Hopkins University Applied Physics labChalmers University of technologyKarolinska InstitutetPenetrating traumatic brain injury (pTBI) has been difficult to model in small laboratory animals, such as rats or mice. Previously, we have established a non-fatal, rat model for penetrating brain injury using a modified air-rifle that accelerates a pellet, which hits a small probe that then penetrates the experimental animal’s brain. Knockout and transgenic strains of mice offer attractive tools to study biological reactions induced by TBI. Hence, in the present study, we adapted and modified our model to be used with mice. The technical characterization of the impact device included depth and speed of impact, as well as dimensions of the temporary cavity formed in a brain surrogate material after impact. Biologically, we have focused on three distinct levels of severity (mild, moderate, and severe), and characterized the acute phase response to injury in terms of tissue destruction, neural degeneration, and gliosis. Functional outcome was assessed by measuring bodyweight and motor performance on rotarod. The results showed that this model is capable of reproducing major morphological and neurological changes of pTBI; as such, we recommend its utilization in research studies aiming to unravel the biological events underlying injury and regeneration after pTBI.http://journal.frontiersin.org/Journal/10.3389/fneur.2014.00209/fullGliosisHistologyTraumatic Brain Injuryneurodegenerationanimal studiesMODELS OF INJURY |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ibolja eCernak Ian D. Wing Johan eDavidsson Stefan ePlantman |
spellingShingle |
Ibolja eCernak Ian D. Wing Johan eDavidsson Stefan ePlantman A Novel Mouse Model of Penetrating Brain Injury Frontiers in Neurology Gliosis Histology Traumatic Brain Injury neurodegeneration animal studies MODELS OF INJURY |
author_facet |
Ibolja eCernak Ian D. Wing Johan eDavidsson Stefan ePlantman |
author_sort |
Ibolja eCernak |
title |
A Novel Mouse Model of Penetrating Brain Injury |
title_short |
A Novel Mouse Model of Penetrating Brain Injury |
title_full |
A Novel Mouse Model of Penetrating Brain Injury |
title_fullStr |
A Novel Mouse Model of Penetrating Brain Injury |
title_full_unstemmed |
A Novel Mouse Model of Penetrating Brain Injury |
title_sort |
novel mouse model of penetrating brain injury |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neurology |
issn |
1664-2295 |
publishDate |
2014-10-01 |
description |
Penetrating traumatic brain injury (pTBI) has been difficult to model in small laboratory animals, such as rats or mice. Previously, we have established a non-fatal, rat model for penetrating brain injury using a modified air-rifle that accelerates a pellet, which hits a small probe that then penetrates the experimental animal’s brain. Knockout and transgenic strains of mice offer attractive tools to study biological reactions induced by TBI. Hence, in the present study, we adapted and modified our model to be used with mice. The technical characterization of the impact device included depth and speed of impact, as well as dimensions of the temporary cavity formed in a brain surrogate material after impact. Biologically, we have focused on three distinct levels of severity (mild, moderate, and severe), and characterized the acute phase response to injury in terms of tissue destruction, neural degeneration, and gliosis. Functional outcome was assessed by measuring bodyweight and motor performance on rotarod. The results showed that this model is capable of reproducing major morphological and neurological changes of pTBI; as such, we recommend its utilization in research studies aiming to unravel the biological events underlying injury and regeneration after pTBI. |
topic |
Gliosis Histology Traumatic Brain Injury neurodegeneration animal studies MODELS OF INJURY |
url |
http://journal.frontiersin.org/Journal/10.3389/fneur.2014.00209/full |
work_keys_str_mv |
AT iboljaecernak anovelmousemodelofpenetratingbraininjury AT iandwing anovelmousemodelofpenetratingbraininjury AT johanedavidsson anovelmousemodelofpenetratingbraininjury AT stefaneplantman anovelmousemodelofpenetratingbraininjury AT iboljaecernak novelmousemodelofpenetratingbraininjury AT iandwing novelmousemodelofpenetratingbraininjury AT johanedavidsson novelmousemodelofpenetratingbraininjury AT stefaneplantman novelmousemodelofpenetratingbraininjury |
_version_ |
1725821960717336576 |