Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics
In recent years, the increase of bacteria antibiotic- resistance has been a severe problem for public health. A useful solution could be to join some phytochemicals naturally present in essential oils (EOs) to the existing antibiotics, with the aim to increase their efficacy in therapies. According...
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doaj-a941bb6af3a2469c8d92e4dbeb61097e2020-11-25T03:19:03ZengMDPI AGAntibiotics2079-63822020-09-01963763710.3390/antibiotics9100637Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and AntibioticsAntonio Rosato0Sabina Sblano1Lara Salvagno2Alessia Carocci3Maria Lisa Clodoveo4Filomena Corbo5Giuseppe Fracchiolla6Department of Pharmacy—Drug Sciences, University of Bari “Aldo Moro”, via E. Orabona, 4-70125 Bari, ItalyDepartment of Pharmacy—Drug Sciences, University of Bari “Aldo Moro”, via E. Orabona, 4-70125 Bari, ItalyDepartment of Pharmacy—Drug Sciences, University of Bari “Aldo Moro”, via E. Orabona, 4-70125 Bari, ItalyDepartment of Pharmacy—Drug Sciences, University of Bari “Aldo Moro”, via E. Orabona, 4-70125 Bari, ItalySchool of Medicine: Interdisciplinary Department of Medicine, University of Bari “Aldo Moro”, piazza G. Cesare, 11-70124 Bari, ItalyDepartment of Pharmacy—Drug Sciences, University of Bari “Aldo Moro”, via E. Orabona, 4-70125 Bari, ItalyDepartment of Pharmacy—Drug Sciences, University of Bari “Aldo Moro”, via E. Orabona, 4-70125 Bari, ItalyIn recent years, the increase of bacteria antibiotic- resistance has been a severe problem for public health. A useful solution could be to join some phytochemicals naturally present in essential oils (EOs) to the existing antibiotics, with the aim to increase their efficacy in therapies. According to in vitro studies, EOs and their components could show such effects. Among them, we studied the activity of <i>Cinnammonum zeylanicum</i>, <i>Mentha piperita</i>, <i>Origanum vulgare</i>, and <i>Thymus vulgaris</i> EOs on bacterial biofilm and their synergism when used in association with some common antibiotics such as norfloxacin, oxacillin, and gentamicin. The chemical composition of EOs was determined using gas chromatography (GC) coupled with mass spectrometry (MS) techniques. The EOs drug efficacy was evaluated on four different strains of Gram-positive bacteria forming biofilms. The synergistic effects were tested through the chequerboard microdilution method. The association EOs-antibiotics showed a strong destruction of the biofilm growth of the four bacterial species considered. The interaction of norfloxacin with EOs was the most effective in all the tested combinations against the strains object of this study. These preliminary results suggest the formulation of a new generation of antimicrobial agents based on a combination of antimicrobial compounds with different origin.https://www.mdpi.com/2079-6382/9/10/637synergismanti-biofilmantibiotic-resistance<i>Mentha piperita</i><i>Cinnamomun zeylanicum</i><i>Origanum vulgare</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Antonio Rosato Sabina Sblano Lara Salvagno Alessia Carocci Maria Lisa Clodoveo Filomena Corbo Giuseppe Fracchiolla |
spellingShingle |
Antonio Rosato Sabina Sblano Lara Salvagno Alessia Carocci Maria Lisa Clodoveo Filomena Corbo Giuseppe Fracchiolla Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics Antibiotics synergism anti-biofilm antibiotic-resistance <i>Mentha piperita</i> <i>Cinnamomun zeylanicum</i> <i>Origanum vulgare</i> |
author_facet |
Antonio Rosato Sabina Sblano Lara Salvagno Alessia Carocci Maria Lisa Clodoveo Filomena Corbo Giuseppe Fracchiolla |
author_sort |
Antonio Rosato |
title |
Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics |
title_short |
Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics |
title_full |
Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics |
title_fullStr |
Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics |
title_full_unstemmed |
Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics |
title_sort |
anti-biofilm inhibitory synergistic effects of combinations of essential oils and antibiotics |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2020-09-01 |
description |
In recent years, the increase of bacteria antibiotic- resistance has been a severe problem for public health. A useful solution could be to join some phytochemicals naturally present in essential oils (EOs) to the existing antibiotics, with the aim to increase their efficacy in therapies. According to in vitro studies, EOs and their components could show such effects. Among them, we studied the activity of <i>Cinnammonum zeylanicum</i>, <i>Mentha piperita</i>, <i>Origanum vulgare</i>, and <i>Thymus vulgaris</i> EOs on bacterial biofilm and their synergism when used in association with some common antibiotics such as norfloxacin, oxacillin, and gentamicin. The chemical composition of EOs was determined using gas chromatography (GC) coupled with mass spectrometry (MS) techniques. The EOs drug efficacy was evaluated on four different strains of Gram-positive bacteria forming biofilms. The synergistic effects were tested through the chequerboard microdilution method. The association EOs-antibiotics showed a strong destruction of the biofilm growth of the four bacterial species considered. The interaction of norfloxacin with EOs was the most effective in all the tested combinations against the strains object of this study. These preliminary results suggest the formulation of a new generation of antimicrobial agents based on a combination of antimicrobial compounds with different origin. |
topic |
synergism anti-biofilm antibiotic-resistance <i>Mentha piperita</i> <i>Cinnamomun zeylanicum</i> <i>Origanum vulgare</i> |
url |
https://www.mdpi.com/2079-6382/9/10/637 |
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