Fructose 1,6‐Bisphosphate as a Protective Agent for Experimental Fat Grafting
Abstract Fat grafting procedures are considered to be a promising regenerative, cell‐directed therapy; however, their survival is mainly influenced by ischemia condition. Fructose 1,6‐bisphosphate (FBP), as an intermediate in energy metabolism, has the potential to rescue cells and tissues from hypo...
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doaj-a9431666a9634b93b098e60f5cc202972020-11-24T21:16:54ZengWileyStem Cells Translational Medicine2157-65642157-65802019-06-018660661610.1002/sctm.18-0212Fructose 1,6‐Bisphosphate as a Protective Agent for Experimental Fat GraftingTao Lv0Yunpeng Gu1Jianhai Bi2Ning Kang3Zhigang Yang4Xin Fu5Qian Wang6Li Yan7Xia Liu8Yilin Cao9Ran Xiao10Research Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaResearch Center of Plastic Surgery Hospital Chinese Academy of Medical Sciences, Peking Union Medical College Beijing People's Republic of ChinaAbstract Fat grafting procedures are considered to be a promising regenerative, cell‐directed therapy; however, their survival is mainly influenced by ischemia condition. Fructose 1,6‐bisphosphate (FBP), as an intermediate in energy metabolism, has the potential to rescue cells and tissues from hypoxic‐ischemic circumstances. In the present study, human lipoaspirates were grafted subcutaneously into nude mice followed by a daily intraperitoneal injection of FBP at different doses for 7 days. Next, the grafts were harvested at different time points till 12 weeks postimplantation and were evaluated for cell viability and function, tissue revascularization and inflammatory cell infiltration using histological analysis, whole‐mount living tissue imaging, glycerol 3‐phosphate dehydrogenase activity assays, and quantitative analysis of gene expression. The results demonstrated that exogenous FBP administration could attenuate the volume and weight reduction of fat graft; meanwhile, FBP enhanced adipocyte viability and function, increased blood vessel formation, and decreased inflammation. Moreover, in vitro cell experiments showed that FBP could promote adipose‐derived stem cell viability and vascular endothelial growth factor (VEGF) mRNA expression in ischemia conditions. Our study indicates that FBP can be used as a protective agent for fat grafting and may be applied in stem cell‐based regenerative medicine. Stem Cells Translational Medicine 2019;8:606–616https://doi.org/10.1002/sctm.18-0212Fat graftingIschemiaVolume retentionFructose 1,6‐bisphosphateAdipose‐derived stem cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tao Lv Yunpeng Gu Jianhai Bi Ning Kang Zhigang Yang Xin Fu Qian Wang Li Yan Xia Liu Yilin Cao Ran Xiao |
spellingShingle |
Tao Lv Yunpeng Gu Jianhai Bi Ning Kang Zhigang Yang Xin Fu Qian Wang Li Yan Xia Liu Yilin Cao Ran Xiao Fructose 1,6‐Bisphosphate as a Protective Agent for Experimental Fat Grafting Stem Cells Translational Medicine Fat grafting Ischemia Volume retention Fructose 1,6‐bisphosphate Adipose‐derived stem cells |
author_facet |
Tao Lv Yunpeng Gu Jianhai Bi Ning Kang Zhigang Yang Xin Fu Qian Wang Li Yan Xia Liu Yilin Cao Ran Xiao |
author_sort |
Tao Lv |
title |
Fructose 1,6‐Bisphosphate as a Protective Agent for Experimental Fat Grafting |
title_short |
Fructose 1,6‐Bisphosphate as a Protective Agent for Experimental Fat Grafting |
title_full |
Fructose 1,6‐Bisphosphate as a Protective Agent for Experimental Fat Grafting |
title_fullStr |
Fructose 1,6‐Bisphosphate as a Protective Agent for Experimental Fat Grafting |
title_full_unstemmed |
Fructose 1,6‐Bisphosphate as a Protective Agent for Experimental Fat Grafting |
title_sort |
fructose 1,6‐bisphosphate as a protective agent for experimental fat grafting |
publisher |
Wiley |
series |
Stem Cells Translational Medicine |
issn |
2157-6564 2157-6580 |
publishDate |
2019-06-01 |
description |
Abstract Fat grafting procedures are considered to be a promising regenerative, cell‐directed therapy; however, their survival is mainly influenced by ischemia condition. Fructose 1,6‐bisphosphate (FBP), as an intermediate in energy metabolism, has the potential to rescue cells and tissues from hypoxic‐ischemic circumstances. In the present study, human lipoaspirates were grafted subcutaneously into nude mice followed by a daily intraperitoneal injection of FBP at different doses for 7 days. Next, the grafts were harvested at different time points till 12 weeks postimplantation and were evaluated for cell viability and function, tissue revascularization and inflammatory cell infiltration using histological analysis, whole‐mount living tissue imaging, glycerol 3‐phosphate dehydrogenase activity assays, and quantitative analysis of gene expression. The results demonstrated that exogenous FBP administration could attenuate the volume and weight reduction of fat graft; meanwhile, FBP enhanced adipocyte viability and function, increased blood vessel formation, and decreased inflammation. Moreover, in vitro cell experiments showed that FBP could promote adipose‐derived stem cell viability and vascular endothelial growth factor (VEGF) mRNA expression in ischemia conditions. Our study indicates that FBP can be used as a protective agent for fat grafting and may be applied in stem cell‐based regenerative medicine. Stem Cells Translational Medicine 2019;8:606–616 |
topic |
Fat grafting Ischemia Volume retention Fructose 1,6‐bisphosphate Adipose‐derived stem cells |
url |
https://doi.org/10.1002/sctm.18-0212 |
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