MicroRNA-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting CD73
Abstract Background MicroRNAs are non-coding RNAs which regulate a variety of cellular functions in the development of tumors. Among the numerous microRNAs, microRNA-30a (miR-30a) is thought to play an important role in the processes of various human tumors. In this study, we aimed to explore the ro...
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doaj-a946f2b8dd5444ebbf80be04a6cbe8282020-11-24T22:00:11ZengBMCBMC Cancer1471-24072017-05-011711910.1186/s12885-017-3291-8MicroRNA-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting CD73Minghao Xie0Huabo Qin1Qianxin Luo2Qunsheng Huang3Xiaosheng He4Zihuan Yang5Ping Lan6Lei Lian7Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen UniversityAbstract Background MicroRNAs are non-coding RNAs which regulate a variety of cellular functions in the development of tumors. Among the numerous microRNAs, microRNA-30a (miR-30a) is thought to play an important role in the processes of various human tumors. In this study, we aimed to explore the role of miR-30a in the process of colorectal cancer (CRC). Methods The quantitative real-time PCR and western blot analysis were used to detect the expressions of miR-30a and CD73 in CRC cell lines and clinical tissues. The luciferase reporter assay was conducted to validate the association between miR-30a and CD73. The CCK-8, terminal deoxynucleotidyl transferase dUTP -biotin nick end labeling (TUNEL) assays and cell cycle flow cytometry were carried out to verify the biological functions of miR-30a in vitro. The nude mouse tumorigenicity experiment was used to clarify the biological role of miR-30a in vivo. Results The expression of miR-30a was significantly reduced in tumor cells and tissues of CRC. The proliferation ability of CRC cells was suppressed and the apoptosis of cells was promoted when miR-30a is over-regulated, however, the biological effects would be inverse since the miR-30a is down-regulated. CD73 is thought to be a target binding gene of miR-30a because miR-30a can bind directly to the 3′-UTR of CD73 mRNA, subsequently reducing its expression. The proliferation suppression of the CRC cells mediated by miR-30a could be rescued after up-regulating the expression of CD73. Conclusions MiR-30a plays an important role on regulating the cell proliferation and apoptosis, thus affecting the growth of the tumor in CRC. And it may participate in the disease process of CRC by regulating the expression of CD73.http://link.springer.com/article/10.1186/s12885-017-3291-8MiR-30aColorectal cancerProliferationApoptosisCD73 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Minghao Xie Huabo Qin Qianxin Luo Qunsheng Huang Xiaosheng He Zihuan Yang Ping Lan Lei Lian |
spellingShingle |
Minghao Xie Huabo Qin Qianxin Luo Qunsheng Huang Xiaosheng He Zihuan Yang Ping Lan Lei Lian MicroRNA-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting CD73 BMC Cancer MiR-30a Colorectal cancer Proliferation Apoptosis CD73 |
author_facet |
Minghao Xie Huabo Qin Qianxin Luo Qunsheng Huang Xiaosheng He Zihuan Yang Ping Lan Lei Lian |
author_sort |
Minghao Xie |
title |
MicroRNA-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting CD73 |
title_short |
MicroRNA-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting CD73 |
title_full |
MicroRNA-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting CD73 |
title_fullStr |
MicroRNA-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting CD73 |
title_full_unstemmed |
MicroRNA-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting CD73 |
title_sort |
microrna-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting cd73 |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2017-05-01 |
description |
Abstract Background MicroRNAs are non-coding RNAs which regulate a variety of cellular functions in the development of tumors. Among the numerous microRNAs, microRNA-30a (miR-30a) is thought to play an important role in the processes of various human tumors. In this study, we aimed to explore the role of miR-30a in the process of colorectal cancer (CRC). Methods The quantitative real-time PCR and western blot analysis were used to detect the expressions of miR-30a and CD73 in CRC cell lines and clinical tissues. The luciferase reporter assay was conducted to validate the association between miR-30a and CD73. The CCK-8, terminal deoxynucleotidyl transferase dUTP -biotin nick end labeling (TUNEL) assays and cell cycle flow cytometry were carried out to verify the biological functions of miR-30a in vitro. The nude mouse tumorigenicity experiment was used to clarify the biological role of miR-30a in vivo. Results The expression of miR-30a was significantly reduced in tumor cells and tissues of CRC. The proliferation ability of CRC cells was suppressed and the apoptosis of cells was promoted when miR-30a is over-regulated, however, the biological effects would be inverse since the miR-30a is down-regulated. CD73 is thought to be a target binding gene of miR-30a because miR-30a can bind directly to the 3′-UTR of CD73 mRNA, subsequently reducing its expression. The proliferation suppression of the CRC cells mediated by miR-30a could be rescued after up-regulating the expression of CD73. Conclusions MiR-30a plays an important role on regulating the cell proliferation and apoptosis, thus affecting the growth of the tumor in CRC. And it may participate in the disease process of CRC by regulating the expression of CD73. |
topic |
MiR-30a Colorectal cancer Proliferation Apoptosis CD73 |
url |
http://link.springer.com/article/10.1186/s12885-017-3291-8 |
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