Utility of basophil activation test for predicting the outcome of wheezing in children: a pilot study
Abstract Background No reliable biological marker for the diagnosis of asthma in younger children is currently available. In this study, we analyzed the differences in basophil activation test (BAT) results among children with recurrent wheezing episodes who had different asthma outcomes. Results A...
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doaj-a94e3ecf233c403fa7619826a0c59a232021-01-10T13:03:03ZengBMCBMC Immunology1471-21722021-01-012211610.1186/s12865-020-00395-4Utility of basophil activation test for predicting the outcome of wheezing in children: a pilot studyJingyang Li0Jinhong Wu1Haipei Liu2Li Hua3Quanhua Liu4Dingzhu Fang5Yi Chen6Ruoxu Ji7Jianhua Zhang8Wenwei Zhong9Department of Pediatrics, Xinhua hospital affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Shanghai Children’s Medical Center affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Xinhua hospital affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Xinhua hospital affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Xinhua hospital affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Xinhua hospital affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Xinhua hospital affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Xinhua hospital affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Xinhua hospital affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Xinhua hospital affiliated to Shanghai Jiao Tong University School of MedicineAbstract Background No reliable biological marker for the diagnosis of asthma in younger children is currently available. In this study, we analyzed the differences in basophil activation test (BAT) results among children with recurrent wheezing episodes who had different asthma outcomes. Results A prospective cohort study was conducted in children aged under 5 years who visited our pediatric respiratory clinic and ward for wheezing. After enrollment, the participants provided samples for a CD63-based BAT performed using an inhalant allergen mixture as a stimulant. Histories of personal allergic diseases and family allergic diseases were evaluated by using a questionnaire. All participants were followed up for 2 years, and their asthma outcomes were evaluated at the end of the follow-up period. The correlation between the BAT results and asthma outcomes was analyzed. Of the 45 originally enrolled children, 38 completed both the follow-up and a BAT. After stimulation with the inhalant mixture, the CD63 expression on basophils and the rate of positive CD63-based BAT results in children diagnosed with asthma were both significantly higher than those in children who were not diagnosed with asthma (p < 0.05 and p < 0.01, respectively). For the prediction of asthma, the positive predictive value and negative predictive value of CD63-based BAT was 71.8 and 69.2%, respectively. The positive likelihood ratio and negative likelihood ratio of CD63-based BAT were 1.70 and 0.3, respectively. Conclusions Our pilot study indicates that CD63-based BAT has potential clinical value for predicting asthma outcome in young children with wheezing episodes.https://doi.org/10.1186/s12865-020-00395-4AsthmaWheezingBasophil activation testCD63Children |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jingyang Li Jinhong Wu Haipei Liu Li Hua Quanhua Liu Dingzhu Fang Yi Chen Ruoxu Ji Jianhua Zhang Wenwei Zhong |
spellingShingle |
Jingyang Li Jinhong Wu Haipei Liu Li Hua Quanhua Liu Dingzhu Fang Yi Chen Ruoxu Ji Jianhua Zhang Wenwei Zhong Utility of basophil activation test for predicting the outcome of wheezing in children: a pilot study BMC Immunology Asthma Wheezing Basophil activation test CD63 Children |
author_facet |
Jingyang Li Jinhong Wu Haipei Liu Li Hua Quanhua Liu Dingzhu Fang Yi Chen Ruoxu Ji Jianhua Zhang Wenwei Zhong |
author_sort |
Jingyang Li |
title |
Utility of basophil activation test for predicting the outcome of wheezing in children: a pilot study |
title_short |
Utility of basophil activation test for predicting the outcome of wheezing in children: a pilot study |
title_full |
Utility of basophil activation test for predicting the outcome of wheezing in children: a pilot study |
title_fullStr |
Utility of basophil activation test for predicting the outcome of wheezing in children: a pilot study |
title_full_unstemmed |
Utility of basophil activation test for predicting the outcome of wheezing in children: a pilot study |
title_sort |
utility of basophil activation test for predicting the outcome of wheezing in children: a pilot study |
publisher |
BMC |
series |
BMC Immunology |
issn |
1471-2172 |
publishDate |
2021-01-01 |
description |
Abstract Background No reliable biological marker for the diagnosis of asthma in younger children is currently available. In this study, we analyzed the differences in basophil activation test (BAT) results among children with recurrent wheezing episodes who had different asthma outcomes. Results A prospective cohort study was conducted in children aged under 5 years who visited our pediatric respiratory clinic and ward for wheezing. After enrollment, the participants provided samples for a CD63-based BAT performed using an inhalant allergen mixture as a stimulant. Histories of personal allergic diseases and family allergic diseases were evaluated by using a questionnaire. All participants were followed up for 2 years, and their asthma outcomes were evaluated at the end of the follow-up period. The correlation between the BAT results and asthma outcomes was analyzed. Of the 45 originally enrolled children, 38 completed both the follow-up and a BAT. After stimulation with the inhalant mixture, the CD63 expression on basophils and the rate of positive CD63-based BAT results in children diagnosed with asthma were both significantly higher than those in children who were not diagnosed with asthma (p < 0.05 and p < 0.01, respectively). For the prediction of asthma, the positive predictive value and negative predictive value of CD63-based BAT was 71.8 and 69.2%, respectively. The positive likelihood ratio and negative likelihood ratio of CD63-based BAT were 1.70 and 0.3, respectively. Conclusions Our pilot study indicates that CD63-based BAT has potential clinical value for predicting asthma outcome in young children with wheezing episodes. |
topic |
Asthma Wheezing Basophil activation test CD63 Children |
url |
https://doi.org/10.1186/s12865-020-00395-4 |
work_keys_str_mv |
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