Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes Mellitus
Background and Aim: Hyperglycemia in type 1 diabetes (T1D) is accompanied by endothelial cell dysfunction which is known to contribute to the pathogenesis of cardiovascular disorders. The aim of the current study was to explore the profile of circulating endothelial progenitor cells (EPCs), circulat...
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doaj-a95a9af9734744beb9fae0df221bbf242020-11-25T04:10:49ZengSAGE PublishingClinical and Applied Thrombosis/Hemostasis1938-27232019-02-012510.1177/1076029618825311Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes MellitusAsmaa M. Zahran MD0Ismail L. Mohamed MD1Osama M. El Asheer MD2Deiaaeldin M. Tamer MD3Mohamed G. M. Abo-ELela MD4Mona H. Abdel-Rahim MD5Omnia H. B. El-Badawy MD6Khalid I. Elsayh MD7 Department of Clinical Pathology, South Egypt Cancer Institute, Assiut, Egypt Pediatric Department, Faculty of Medicine, Assiut University, Assiut, Egypt Pediatric Department, Faculty of Medicine, Assiut University, Assiut, Egypt Pediatric Department, Faculty of Medicine, Assiut University, Assiut, Egypt Pediatric Department, Faculty of Medicine, Assiut University, Assiut, Egypt Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt Pediatric Department, Faculty of Medicine, Assiut University, Assiut, EgyptBackground and Aim: Hyperglycemia in type 1 diabetes (T1D) is accompanied by endothelial cell dysfunction which is known to contribute to the pathogenesis of cardiovascular disorders. The aim of the current study was to explore the profile of circulating endothelial progenitor cells (EPCs), circulating endothelial cells (CECs), endothelial and platelet derived micropaticles (EMPs, PMPs) and total microparticles (TMPs), in T1D children in relation to each other and to the metabolic disorders accompanying T1D. Patients and Methods: Thirty T1D patients and 20 age and sex matched healthy volunteers were assessed for HbA1c level and lipid profile. Quantification of CECs, EPCs, TMPs, EMPs and PMPs was done by flow cytometry. Results: The mean levels of EMPs, PMPs, TMPs and CECs were significantly higher in diabetic children compared to controls. Meanwhile, the levels of EPCs were significantly lower in diabetic children compared to controls. Both PMPs and CECs showed the highest significant differences between patients and controls and their levels were directly related to HbA1c, total cholesterol, LDL and triglycerides. A moderate correlation was observed between the frequency of PMPs and CECs. EPCs revealed negative correlations with both LDL and triglycerides. TMPs were only related to LDL, while EMPs were only related to HbA1c. Conclusion: Although there is disturbance in the levels of EMPs, PMPs, TMPs, CECs and EPCs in type 1 diabetic children compared to the controls, only the levels of PMPs and CECs were closely affected by the poor glycemic control and dyslipidemia occurring in T1D; thus may contribute to a higher risk of cardiovascular diseases.https://doi.org/10.1177/1076029618825311 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Asmaa M. Zahran MD Ismail L. Mohamed MD Osama M. El Asheer MD Deiaaeldin M. Tamer MD Mohamed G. M. Abo-ELela MD Mona H. Abdel-Rahim MD Omnia H. B. El-Badawy MD Khalid I. Elsayh MD |
spellingShingle |
Asmaa M. Zahran MD Ismail L. Mohamed MD Osama M. El Asheer MD Deiaaeldin M. Tamer MD Mohamed G. M. Abo-ELela MD Mona H. Abdel-Rahim MD Omnia H. B. El-Badawy MD Khalid I. Elsayh MD Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes Mellitus Clinical and Applied Thrombosis/Hemostasis |
author_facet |
Asmaa M. Zahran MD Ismail L. Mohamed MD Osama M. El Asheer MD Deiaaeldin M. Tamer MD Mohamed G. M. Abo-ELela MD Mona H. Abdel-Rahim MD Omnia H. B. El-Badawy MD Khalid I. Elsayh MD |
author_sort |
Asmaa M. Zahran MD |
title |
Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes Mellitus |
title_short |
Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes Mellitus |
title_full |
Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes Mellitus |
title_fullStr |
Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes Mellitus |
title_full_unstemmed |
Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes Mellitus |
title_sort |
circulating endothelial cells, circulating endothelial progenitor cells, and circulating microparticles in type 1 diabetes mellitus |
publisher |
SAGE Publishing |
series |
Clinical and Applied Thrombosis/Hemostasis |
issn |
1938-2723 |
publishDate |
2019-02-01 |
description |
Background and Aim: Hyperglycemia in type 1 diabetes (T1D) is accompanied by endothelial cell dysfunction which is known to contribute to the pathogenesis of cardiovascular disorders. The aim of the current study was to explore the profile of circulating endothelial progenitor cells (EPCs), circulating endothelial cells (CECs), endothelial and platelet derived micropaticles (EMPs, PMPs) and total microparticles (TMPs), in T1D children in relation to each other and to the metabolic disorders accompanying T1D. Patients and Methods: Thirty T1D patients and 20 age and sex matched healthy volunteers were assessed for HbA1c level and lipid profile. Quantification of CECs, EPCs, TMPs, EMPs and PMPs was done by flow cytometry. Results: The mean levels of EMPs, PMPs, TMPs and CECs were significantly higher in diabetic children compared to controls. Meanwhile, the levels of EPCs were significantly lower in diabetic children compared to controls. Both PMPs and CECs showed the highest significant differences between patients and controls and their levels were directly related to HbA1c, total cholesterol, LDL and triglycerides. A moderate correlation was observed between the frequency of PMPs and CECs. EPCs revealed negative correlations with both LDL and triglycerides. TMPs were only related to LDL, while EMPs were only related to HbA1c. Conclusion: Although there is disturbance in the levels of EMPs, PMPs, TMPs, CECs and EPCs in type 1 diabetic children compared to the controls, only the levels of PMPs and CECs were closely affected by the poor glycemic control and dyslipidemia occurring in T1D; thus may contribute to a higher risk of cardiovascular diseases. |
url |
https://doi.org/10.1177/1076029618825311 |
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