CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis
Background. Pyroptosis, a novel form of inflammatory programmed cell death, was recently found to be a cause of mucosal barrier defect. In our pervious study, CD147 expression was documented to increase in intestinal tissue of inflammatory bowel disease (IBD). Objective. The aim of this study was to...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2020-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2020/5341247 |
id |
doaj-a95f2ad9a7fd4dee810aa52c85835db7 |
---|---|
record_format |
Article |
spelling |
doaj-a95f2ad9a7fd4dee810aa52c85835db72020-11-25T02:59:58ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/53412475341247CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated PyroptosisZhaohui Xu0Ruitao Liu1Ling Huang2Yuxin Xu3Mingmin Su4Jiayu Chen5Lanlan Geng6Wanfu Xu7Sitang Gong8The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaDepartment of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou 350122, ChinaDepartment of Cancer Biology and Therapeutics, School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Wales CF103AT, UKDepartment of Neonatal Intensive Care Unit, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaThe First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, ChinaBackground. Pyroptosis, a novel form of inflammatory programmed cell death, was recently found to be a cause of mucosal barrier defect. In our pervious study, CD147 expression was documented to increase in intestinal tissue of inflammatory bowel disease (IBD). Objective. The aim of this study was to determine the function of serum CD147 in pyroptosis. Methods. The study group consisted of 96 cases. The centration of CD147, IL-1β, and IL-18 levels in serum was assessed by ELISA. Real-time PCR and WB were performed to analyze the effect of CD147 on pyroptosis. Results. In this study, our results showed that CD147 induced cell pyroptosis in intestinal epithelial cells (IECs) by enhancement of IL-1β and IL-18 expression and secretion in IECs, which is attributed to activation of inflammasomes, including caspase-1 and GSDMD as well as GSDME, leading to aggregate inflammatory reaction. Mechanically, CD147 promoted phosphorylation of NF-κB p65 in IECs, while inhibition of NF-κB activity by the NF-κB inhibitor BAY11-7082 reversed the effect of CD147 on IL-1β and IL-18 secretion. Most importantly, serum CD147 level is slightly clinically correlated with IL-1β, but not IL-18 level. Conclusion. These findings revealed a critical role of CD147 in the patients with IBD, suggesting that blockade of CD147 may be a novel therapeutic strategy for the patients with IBD.http://dx.doi.org/10.1155/2020/5341247 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhaohui Xu Ruitao Liu Ling Huang Yuxin Xu Mingmin Su Jiayu Chen Lanlan Geng Wanfu Xu Sitang Gong |
spellingShingle |
Zhaohui Xu Ruitao Liu Ling Huang Yuxin Xu Mingmin Su Jiayu Chen Lanlan Geng Wanfu Xu Sitang Gong CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis BioMed Research International |
author_facet |
Zhaohui Xu Ruitao Liu Ling Huang Yuxin Xu Mingmin Su Jiayu Chen Lanlan Geng Wanfu Xu Sitang Gong |
author_sort |
Zhaohui Xu |
title |
CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis |
title_short |
CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis |
title_full |
CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis |
title_fullStr |
CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis |
title_full_unstemmed |
CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis |
title_sort |
cd147 aggravated inflammatory bowel disease by triggering nf-κb-mediated pyroptosis |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
Background. Pyroptosis, a novel form of inflammatory programmed cell death, was recently found to be a cause of mucosal barrier defect. In our pervious study, CD147 expression was documented to increase in intestinal tissue of inflammatory bowel disease (IBD). Objective. The aim of this study was to determine the function of serum CD147 in pyroptosis. Methods. The study group consisted of 96 cases. The centration of CD147, IL-1β, and IL-18 levels in serum was assessed by ELISA. Real-time PCR and WB were performed to analyze the effect of CD147 on pyroptosis. Results. In this study, our results showed that CD147 induced cell pyroptosis in intestinal epithelial cells (IECs) by enhancement of IL-1β and IL-18 expression and secretion in IECs, which is attributed to activation of inflammasomes, including caspase-1 and GSDMD as well as GSDME, leading to aggregate inflammatory reaction. Mechanically, CD147 promoted phosphorylation of NF-κB p65 in IECs, while inhibition of NF-κB activity by the NF-κB inhibitor BAY11-7082 reversed the effect of CD147 on IL-1β and IL-18 secretion. Most importantly, serum CD147 level is slightly clinically correlated with IL-1β, but not IL-18 level. Conclusion. These findings revealed a critical role of CD147 in the patients with IBD, suggesting that blockade of CD147 may be a novel therapeutic strategy for the patients with IBD. |
url |
http://dx.doi.org/10.1155/2020/5341247 |
work_keys_str_mv |
AT zhaohuixu cd147aggravatedinflammatoryboweldiseasebytriggeringnfkbmediatedpyroptosis AT ruitaoliu cd147aggravatedinflammatoryboweldiseasebytriggeringnfkbmediatedpyroptosis AT linghuang cd147aggravatedinflammatoryboweldiseasebytriggeringnfkbmediatedpyroptosis AT yuxinxu cd147aggravatedinflammatoryboweldiseasebytriggeringnfkbmediatedpyroptosis AT mingminsu cd147aggravatedinflammatoryboweldiseasebytriggeringnfkbmediatedpyroptosis AT jiayuchen cd147aggravatedinflammatoryboweldiseasebytriggeringnfkbmediatedpyroptosis AT lanlangeng cd147aggravatedinflammatoryboweldiseasebytriggeringnfkbmediatedpyroptosis AT wanfuxu cd147aggravatedinflammatoryboweldiseasebytriggeringnfkbmediatedpyroptosis AT sitanggong cd147aggravatedinflammatoryboweldiseasebytriggeringnfkbmediatedpyroptosis |
_version_ |
1715332202469785600 |