Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation
Inflammatory reactions mediated by the NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome contributes to non-small-cell lung cancer (NSCLC) progression, particularly in patients with bacterial infections. Salidroside (SAL) has recently been shown to suppress lipopolysaccharide- (LP...
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Series: | Oxidative Medicine and Cellular Longevity |
Online Access: | http://dx.doi.org/10.1155/2021/6614574 |
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doaj-a95fb64d6c8f49f8b4e55fea4a7134872021-08-30T00:00:52ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09942021-01-01202110.1155/2021/6614574Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome RegulationWeidong Ma0Ziyuan Wang1Yan Zhao2Qibin Wang3Yonghong Zhang4Pan Lei5Wei Lu6Shan Yan7Jun Zhou8Xiaojiao Li9Wenjun Yu10Yaoxin Zhong11Li Chen12Tao Zheng13Institute of Wudang Traditional Chinese MedicineInstitute of Wudang Traditional Chinese MedicineInstitute of Wudang Traditional Chinese MedicineDepartment of PharmacyInstitute of Wudang Traditional Chinese MedicineInstitute of Wudang Traditional Chinese MedicineDepartment of PharmacyInstitute of Wudang Traditional Chinese MedicineInstitute of Wudang Traditional Chinese MedicineInstitute of Wudang Traditional Chinese MedicineInstitute of Wudang Traditional Chinese MedicineInstitute of Wudang Traditional Chinese MedicineInstitute of Wudang Traditional Chinese MedicineInstitute of Wudang Traditional Chinese MedicineInflammatory reactions mediated by the NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome contributes to non-small-cell lung cancer (NSCLC) progression, particularly in patients with bacterial infections. Salidroside (SAL) has recently been shown to suppress lipopolysaccharide- (LPS-) induced NSCLC proliferation and migration, but its mechanism of action remains unclear. It has been shown that SAL improves metabolic inflammation in diabetic rodents through AMP-activated protein kinase- (AMPK-) dependent inhibition of the NLRP3 inflammasome. However, whether the NLRP3 inflammasome is regulated by SAL in NSCLC cells and how its underlying mechanism(s) can be determined require clarification. In this study, human lung alveolar basal carcinoma epithelial (A549) cells were treated with LPS, and the effects of SAL on cell proliferation, migration, AMPK activity, reactive oxygen species (ROS) production, and NLRP3 inflammasome activation were investigated. We found that LPS induction increases the proliferation and migration of A549 cells which was suppressed by SAL. Moreover, SAL protected A549 cells against LPS-induced AMPK inhibition, ROS production, and NLRP3 inflammasome activation. Blocking AMPK using Compound C almost completely suppressed the beneficial effects of SAL. In summary, these results indicate that SAL suppresses the proliferation and migration of human lung cancer cells through AMPK-dependent NLRP3 inflammasome regulation.http://dx.doi.org/10.1155/2021/6614574 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Weidong Ma Ziyuan Wang Yan Zhao Qibin Wang Yonghong Zhang Pan Lei Wei Lu Shan Yan Jun Zhou Xiaojiao Li Wenjun Yu Yaoxin Zhong Li Chen Tao Zheng |
spellingShingle |
Weidong Ma Ziyuan Wang Yan Zhao Qibin Wang Yonghong Zhang Pan Lei Wei Lu Shan Yan Jun Zhou Xiaojiao Li Wenjun Yu Yaoxin Zhong Li Chen Tao Zheng Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation Oxidative Medicine and Cellular Longevity |
author_facet |
Weidong Ma Ziyuan Wang Yan Zhao Qibin Wang Yonghong Zhang Pan Lei Wei Lu Shan Yan Jun Zhou Xiaojiao Li Wenjun Yu Yaoxin Zhong Li Chen Tao Zheng |
author_sort |
Weidong Ma |
title |
Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation |
title_short |
Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation |
title_full |
Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation |
title_fullStr |
Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation |
title_full_unstemmed |
Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK-Dependent NLRP3 Inflammasome Regulation |
title_sort |
salidroside suppresses the proliferation and migration of human lung cancer cells through ampk-dependent nlrp3 inflammasome regulation |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0994 |
publishDate |
2021-01-01 |
description |
Inflammatory reactions mediated by the NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome contributes to non-small-cell lung cancer (NSCLC) progression, particularly in patients with bacterial infections. Salidroside (SAL) has recently been shown to suppress lipopolysaccharide- (LPS-) induced NSCLC proliferation and migration, but its mechanism of action remains unclear. It has been shown that SAL improves metabolic inflammation in diabetic rodents through AMP-activated protein kinase- (AMPK-) dependent inhibition of the NLRP3 inflammasome. However, whether the NLRP3 inflammasome is regulated by SAL in NSCLC cells and how its underlying mechanism(s) can be determined require clarification. In this study, human lung alveolar basal carcinoma epithelial (A549) cells were treated with LPS, and the effects of SAL on cell proliferation, migration, AMPK activity, reactive oxygen species (ROS) production, and NLRP3 inflammasome activation were investigated. We found that LPS induction increases the proliferation and migration of A549 cells which was suppressed by SAL. Moreover, SAL protected A549 cells against LPS-induced AMPK inhibition, ROS production, and NLRP3 inflammasome activation. Blocking AMPK using Compound C almost completely suppressed the beneficial effects of SAL. In summary, these results indicate that SAL suppresses the proliferation and migration of human lung cancer cells through AMPK-dependent NLRP3 inflammasome regulation. |
url |
http://dx.doi.org/10.1155/2021/6614574 |
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