Oxidation- and Temperature-Responsive Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) Micelle as a Potential Anticancer Drug Carrier
Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) (P(HEA-<i>co</i>-PVS)), as an oxidizable amphiphilic polymer, was prepared for the fabrication of an oxidation- and temperature-responsive micelle for the delivery of doxorubicin (DOX). The interfacial activity of H&l...
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doaj-a96030682dc44092b23eabc043de42792020-11-25T02:42:11ZengMDPI AGPharmaceutics1999-49232019-09-0111946210.3390/pharmaceutics11090462pharmaceutics11090462Oxidation- and Temperature-Responsive Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) Micelle as a Potential Anticancer Drug CarrierTae Hoon Kim0Madhusudhan Alle1Jin-Chul Kim2Department of Medical Biomaterials Engineering, College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2 dong, Chuncheon 200-701, Kangwon-do, KoreaDepartment of Medical Biomaterials Engineering, College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2 dong, Chuncheon 200-701, Kangwon-do, KoreaDepartment of Medical Biomaterials Engineering, College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2 dong, Chuncheon 200-701, Kangwon-do, KoreaPoly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) (P(HEA-<i>co</i>-PVS)), as an oxidizable amphiphilic polymer, was prepared for the fabrication of an oxidation- and temperature-responsive micelle for the delivery of doxorubicin (DOX). The interfacial activity of H<sub>2</sub>O<sub>2</sub>-treated P(HEA-<i>co</i>-PVS) was significantly lower than that of the untreated variety, possibly because of the oxidization of PVS. P(HEA-<i>co</i>-PVS) exhibited a lower critical solution temperature (LCST) behavior and the LCST increased upon H<sub>2</sub>O<sub>2</sub> treatment. The copolymer micelles, prepared by the dialysis method, were found to be round particles (less than 100 nm) on TEM micrograph. The release degree of Nile red loaded in the micelles was higher when the H<sub>2</sub>O<sub>2</sub> concentration was higher, possibly because the micelles could be solubilized more readily at a higher H<sub>2</sub>O<sub>2</sub> concentration. The release degree was more strongly dependent on the oxidizing agent concentration when the temperature was higher. DOX loaded in the micelles suppressed the in vitro growth of KB cells (a human cancer cell type originating from the cervix) much more effectively than DOX loaded in an unoxidizable control micelle and free DOX, possibly because the copolymer would undergo an increase in its LCST, lose its amphiphilic property, and the micelles would be disassembled. The DOX-loaded micelles were readily internalized into KB cells, as evidenced by flow cytometry (FACS) and confocal laser scanning microscopy (CLSM).https://www.mdpi.com/1999-4923/11/9/462poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide)oxidizable amphiphilic polymermicelledoxorubicinoxidation- and temperature-responsive releaseanticancer efficacy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tae Hoon Kim Madhusudhan Alle Jin-Chul Kim |
spellingShingle |
Tae Hoon Kim Madhusudhan Alle Jin-Chul Kim Oxidation- and Temperature-Responsive Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) Micelle as a Potential Anticancer Drug Carrier Pharmaceutics poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) oxidizable amphiphilic polymer micelle doxorubicin oxidation- and temperature-responsive release anticancer efficacy |
author_facet |
Tae Hoon Kim Madhusudhan Alle Jin-Chul Kim |
author_sort |
Tae Hoon Kim |
title |
Oxidation- and Temperature-Responsive Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) Micelle as a Potential Anticancer Drug Carrier |
title_short |
Oxidation- and Temperature-Responsive Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) Micelle as a Potential Anticancer Drug Carrier |
title_full |
Oxidation- and Temperature-Responsive Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) Micelle as a Potential Anticancer Drug Carrier |
title_fullStr |
Oxidation- and Temperature-Responsive Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) Micelle as a Potential Anticancer Drug Carrier |
title_full_unstemmed |
Oxidation- and Temperature-Responsive Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) Micelle as a Potential Anticancer Drug Carrier |
title_sort |
oxidation- and temperature-responsive poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) micelle as a potential anticancer drug carrier |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2019-09-01 |
description |
Poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) (P(HEA-<i>co</i>-PVS)), as an oxidizable amphiphilic polymer, was prepared for the fabrication of an oxidation- and temperature-responsive micelle for the delivery of doxorubicin (DOX). The interfacial activity of H<sub>2</sub>O<sub>2</sub>-treated P(HEA-<i>co</i>-PVS) was significantly lower than that of the untreated variety, possibly because of the oxidization of PVS. P(HEA-<i>co</i>-PVS) exhibited a lower critical solution temperature (LCST) behavior and the LCST increased upon H<sub>2</sub>O<sub>2</sub> treatment. The copolymer micelles, prepared by the dialysis method, were found to be round particles (less than 100 nm) on TEM micrograph. The release degree of Nile red loaded in the micelles was higher when the H<sub>2</sub>O<sub>2</sub> concentration was higher, possibly because the micelles could be solubilized more readily at a higher H<sub>2</sub>O<sub>2</sub> concentration. The release degree was more strongly dependent on the oxidizing agent concentration when the temperature was higher. DOX loaded in the micelles suppressed the in vitro growth of KB cells (a human cancer cell type originating from the cervix) much more effectively than DOX loaded in an unoxidizable control micelle and free DOX, possibly because the copolymer would undergo an increase in its LCST, lose its amphiphilic property, and the micelles would be disassembled. The DOX-loaded micelles were readily internalized into KB cells, as evidenced by flow cytometry (FACS) and confocal laser scanning microscopy (CLSM). |
topic |
poly(hydroxyethyl acrylate-<i>co</i>-phenyl vinyl sulfide) oxidizable amphiphilic polymer micelle doxorubicin oxidation- and temperature-responsive release anticancer efficacy |
url |
https://www.mdpi.com/1999-4923/11/9/462 |
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