Proteomic dataset of the organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 grown on hexachlorobenzene as electron acceptor

The proteome of the anaerobic organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 was analyzed by nano liquid chromatography coupled to mass spectrometry (LC-MS/MS). Two different preparation methods, (i) in-solution and (ii) in-gel proteolytic digestion were assessed to elucidate...

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Main Authors: Christian L. Schiffmann, Wolfgang Otto, Rasmus Hansen, Per Halkjær Nielsen, Lorenz Adrian, Jana Seifert, Martin von Bergen, Nico Jehmlich
Format: Article
Language:English
Published: Elsevier 2016-06-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916300592
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spelling doaj-a9848f4f7eb14c9ba26eda35593bbf782020-11-25T03:35:16ZengElsevierData in Brief2352-34092016-06-017253256Proteomic dataset of the organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 grown on hexachlorobenzene as electron acceptorChristian L. Schiffmann0Wolfgang Otto1Rasmus Hansen2Per Halkjær Nielsen3Lorenz Adrian4Jana Seifert5Martin von Bergen6Nico Jehmlich7Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, GermanyDepartment of Molecular Systems Biology, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, GermanyAalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Sohngårdsholmsvej 49, 9000 Aalborg, DenmarkAalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Sohngårdsholmsvej 49, 9000 Aalborg, DenmarkDepartment Isotope Biogeochemistry, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, GermanyInstitute of Animal Science, Hohenheim University, 70593 Stuttgart, GermanyDepartment of Molecular Systems Biology, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, Germany; Aalborg University, Department of Biotechnology, Chemistry and Environmental Engineering, Sohngårdsholmsvej 49, 9000 Aalborg, DenmarkDepartment of Molecular Systems Biology, Helmholtz Centre for Environmental Research–UFZ, Permoserstr. 15, 04318 Leipzig, Germany; Corresponding author. Tel.: +49 341 235 4767; fax: +49 341 235 450823.The proteome of the anaerobic organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 was analyzed by nano liquid chromatography coupled to mass spectrometry (LC-MS/MS). Two different preparation methods, (i) in-solution and (ii) in-gel proteolytic digestion were assessed to elucidate the core and the functional proteome of bacterial cultures grown in synthetic anaerobic medium with hexachlorobenzene as sole electron acceptor. A detailed analysis of the data presented is available (Schiffmann et al., 2014) [1]. Keywords: Proteomic reference profile, Dehalococcoides mccartyi strain, CBDB1, Shotgun proteomics, Spectral libraryhttp://www.sciencedirect.com/science/article/pii/S2352340916300592
collection DOAJ
language English
format Article
sources DOAJ
author Christian L. Schiffmann
Wolfgang Otto
Rasmus Hansen
Per Halkjær Nielsen
Lorenz Adrian
Jana Seifert
Martin von Bergen
Nico Jehmlich
spellingShingle Christian L. Schiffmann
Wolfgang Otto
Rasmus Hansen
Per Halkjær Nielsen
Lorenz Adrian
Jana Seifert
Martin von Bergen
Nico Jehmlich
Proteomic dataset of the organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 grown on hexachlorobenzene as electron acceptor
Data in Brief
author_facet Christian L. Schiffmann
Wolfgang Otto
Rasmus Hansen
Per Halkjær Nielsen
Lorenz Adrian
Jana Seifert
Martin von Bergen
Nico Jehmlich
author_sort Christian L. Schiffmann
title Proteomic dataset of the organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 grown on hexachlorobenzene as electron acceptor
title_short Proteomic dataset of the organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 grown on hexachlorobenzene as electron acceptor
title_full Proteomic dataset of the organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 grown on hexachlorobenzene as electron acceptor
title_fullStr Proteomic dataset of the organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 grown on hexachlorobenzene as electron acceptor
title_full_unstemmed Proteomic dataset of the organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 grown on hexachlorobenzene as electron acceptor
title_sort proteomic dataset of the organohalide-respiring bacterium dehalococcoides mccartyi strain cbdb1 grown on hexachlorobenzene as electron acceptor
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2016-06-01
description The proteome of the anaerobic organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 was analyzed by nano liquid chromatography coupled to mass spectrometry (LC-MS/MS). Two different preparation methods, (i) in-solution and (ii) in-gel proteolytic digestion were assessed to elucidate the core and the functional proteome of bacterial cultures grown in synthetic anaerobic medium with hexachlorobenzene as sole electron acceptor. A detailed analysis of the data presented is available (Schiffmann et al., 2014) [1]. Keywords: Proteomic reference profile, Dehalococcoides mccartyi strain, CBDB1, Shotgun proteomics, Spectral library
url http://www.sciencedirect.com/science/article/pii/S2352340916300592
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