Essential Oil Composition, Total Phenol Compounds and Antioxidant Activity of Hypericum perforatum L. Extract Collected from North of Iran
Background: St. John’s Wort (Hypericum perforatum L.), as the most important species of the genus, has been used in traditional medicine for thousands of years. Recently, the plant compounds are used to treat mild and moderate depression around the world. Objective: This study identified the con...
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doaj-a98e7a5035e94da2b18654110eacf4af2021-09-19T04:26:56ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582012-02-011141218226Essential Oil Composition, Total Phenol Compounds and Antioxidant Activity of Hypericum perforatum L. Extract Collected from North of IranMR Morshedloo0A Ebadi1MR Fatahi Moghaddam2D Yazdani3 1- Department of Horticulture, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj 1- Department of Horticulture, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj 1- Department of Horticulture, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj 2- Department of Pharmacognosy & Pharmacy, Institute of Medicinal Plants, ACECR Background: St. John’s Wort (Hypericum perforatum L.), as the most important species of the genus, has been used in traditional medicine for thousands of years. Recently, the plant compounds are used to treat mild and moderate depression around the world. Objective: This study identified the constituents of essential oil and total phenolic compounds and also antioxidant activity of methanol extract of H. perforatum collected from wildly growing area in the north of Iran (Tonecabon, Mazandaran province) . Methods: Essential oil was extracted by hydro-distillation of air dried flowering shoots using Clevenger apparatus. The essential oils were analyzed by both gas chromatography (GC) and gas chromatography −mass spectrometry (GC-MS). Total phenolic compounds in the methanol (80%) extract were determined according to the Folin-Ciocalteu reagent using tannic acid as standard. Antioxidant activity of extract was also evaluated by DPPH free radical method. Results: Monoterpens (25.63%) were found to be the major fraction of the oil and α- Pinene (21.88%) was found to be the most abundant compound, followed by nonane (9.77%), n- octane (9.13%) and dodecanol (6.80%). In addition, the results indicated total phenol content (50 μg gallic acid equivalent/g) and antioxidant activity (34. 37 μg/ml) of methanolic extract. Conclusion: α - Pinene was the major constituent of essential oil of H. perforatum tested sample and its methanol extract contained the high amount of total phenolic compounds and consequently exhibited a considerable antioxidant activity.http://jmp.ir/article-1-493-en.htmlhypericum perforatumessential oilmonoterpensα- pinene antioxidant activitytotal phenolics content |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
MR Morshedloo A Ebadi MR Fatahi Moghaddam D Yazdani |
spellingShingle |
MR Morshedloo A Ebadi MR Fatahi Moghaddam D Yazdani Essential Oil Composition, Total Phenol Compounds and Antioxidant Activity of Hypericum perforatum L. Extract Collected from North of Iran Journal of Medicinal Plants hypericum perforatum essential oil monoterpens α- pinene antioxidant activity total phenolics content |
author_facet |
MR Morshedloo A Ebadi MR Fatahi Moghaddam D Yazdani |
author_sort |
MR Morshedloo |
title |
Essential Oil Composition, Total Phenol Compounds and Antioxidant Activity of Hypericum perforatum L. Extract Collected from North of Iran |
title_short |
Essential Oil Composition, Total Phenol Compounds and Antioxidant Activity of Hypericum perforatum L. Extract Collected from North of Iran |
title_full |
Essential Oil Composition, Total Phenol Compounds and Antioxidant Activity of Hypericum perforatum L. Extract Collected from North of Iran |
title_fullStr |
Essential Oil Composition, Total Phenol Compounds and Antioxidant Activity of Hypericum perforatum L. Extract Collected from North of Iran |
title_full_unstemmed |
Essential Oil Composition, Total Phenol Compounds and Antioxidant Activity of Hypericum perforatum L. Extract Collected from North of Iran |
title_sort |
essential oil composition, total phenol compounds and antioxidant activity of hypericum perforatum l. extract collected from north of iran |
publisher |
Institue of Medicinal Plants, ACECR |
series |
Journal of Medicinal Plants |
issn |
2717-204X 2717-2058 |
publishDate |
2012-02-01 |
description |
Background: St. John’s Wort (Hypericum perforatum L.), as the most important species of the genus, has been used in traditional medicine for thousands of years. Recently, the plant compounds are used to treat mild and moderate depression around the world.
Objective: This study identified the constituents of essential oil and total phenolic compounds and also antioxidant activity of methanol extract of H. perforatum collected from wildly growing area in the north of Iran (Tonecabon, Mazandaran province) .
Methods: Essential oil was extracted by hydro-distillation of air dried flowering shoots using Clevenger apparatus. The essential oils were analyzed by both gas chromatography (GC) and gas chromatography −mass spectrometry (GC-MS). Total phenolic compounds in the methanol (80%) extract were determined according to the Folin-Ciocalteu reagent using tannic acid as standard. Antioxidant activity of extract was also evaluated by DPPH free radical method.
Results: Monoterpens (25.63%) were found to be the major fraction of the oil and
α- Pinene (21.88%) was found to be the most abundant compound, followed by nonane (9.77%), n- octane (9.13%) and dodecanol (6.80%). In addition, the results indicated total phenol content (50 μg gallic acid equivalent/g) and antioxidant activity (34. 37 μg/ml) of methanolic extract.
Conclusion: α - Pinene was the major constituent of essential oil of H. perforatum tested sample and its methanol extract contained the high amount of total phenolic compounds and consequently exhibited a considerable antioxidant activity. |
topic |
hypericum perforatum essential oil monoterpens α- pinene antioxidant activity total phenolics content |
url |
http://jmp.ir/article-1-493-en.html |
work_keys_str_mv |
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