Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, China
Listeria monocytogenes is among the most important foodborne pathogens, which can be isolated from bird feces. The aim of this study was to examine the prevalence, genotypes, virulence factors and drug-resistance characteristics of L. monocytogenes isolated from the black-headed gull in Kunming, Chi...
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doaj-a99aa5dcbf734b08a758103cf53d2b602020-11-25T01:20:29ZengElsevierJournal of Infection and Public Health1876-03412018-01-011115963Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, ChinaXiaolong Cao0Yi Wang1Yan Wang2Changyun Ye3Department of Microbiology, Guizhou Medical University, Guiyang, Guizhou 550004, China; State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China; Corresponding author.Listeria monocytogenes is among the most important foodborne pathogens, which can be isolated from bird feces. The aim of this study was to examine the prevalence, genotypes, virulence factors and drug-resistance characteristics of L. monocytogenes isolated from the black-headed gull in Kunming, China. Serotypes and virulence genes were identified by polymerase chain reaction. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) methods were used to describe the genetic characteristics of all positive isolates. Furthermore, all L. monocytogenes isolates were tested for antimicrobial susceptibility by disk diffusion method. All isolates were positive for inlA, inlB, plcB, prfA, iap, actA and hly genes. Serotyping showed the isolates belonged to serotype 1/2b (2/9, 22%), 1/2c (3/9, 33%), and 4a (4/9, 44%). PFGE showed 4 different pulsotypes (PTs), and MLST differentiated the 9 isolates into 4 sequence types (STs), ST3 (1), ST5 (1), ST35 (3) and ST201 (4). The result of antimicrobial susceptibility revealed that all isolates were naturally sensitive to most antibiotics. The L. monocytogenes isolates had pathogenic potential. There is a potential infection risk for people who come to frequent contact with black-headed gulls in Kunming. Hence, it is necessary to conduct surveillance of L. monocytogenes in the migratory black-headed gulls, which would be valuable to prevent listeriosis in local residents and tourists. Keywords: Listeria monocytogenes, Virulence gene, PFGE, MLST, Antibiotic resistancehttp://www.sciencedirect.com/science/article/pii/S1876034117300953 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaolong Cao Yi Wang Yan Wang Changyun Ye |
spellingShingle |
Xiaolong Cao Yi Wang Yan Wang Changyun Ye Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, China Journal of Infection and Public Health |
author_facet |
Xiaolong Cao Yi Wang Yan Wang Changyun Ye |
author_sort |
Xiaolong Cao |
title |
Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, China |
title_short |
Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, China |
title_full |
Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, China |
title_fullStr |
Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, China |
title_full_unstemmed |
Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, China |
title_sort |
isolation and characterization of listeria monocytogenes from the black-headed gull feces in kunming, china |
publisher |
Elsevier |
series |
Journal of Infection and Public Health |
issn |
1876-0341 |
publishDate |
2018-01-01 |
description |
Listeria monocytogenes is among the most important foodborne pathogens, which can be isolated from bird feces. The aim of this study was to examine the prevalence, genotypes, virulence factors and drug-resistance characteristics of L. monocytogenes isolated from the black-headed gull in Kunming, China. Serotypes and virulence genes were identified by polymerase chain reaction. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) methods were used to describe the genetic characteristics of all positive isolates. Furthermore, all L. monocytogenes isolates were tested for antimicrobial susceptibility by disk diffusion method. All isolates were positive for inlA, inlB, plcB, prfA, iap, actA and hly genes. Serotyping showed the isolates belonged to serotype 1/2b (2/9, 22%), 1/2c (3/9, 33%), and 4a (4/9, 44%). PFGE showed 4 different pulsotypes (PTs), and MLST differentiated the 9 isolates into 4 sequence types (STs), ST3 (1), ST5 (1), ST35 (3) and ST201 (4). The result of antimicrobial susceptibility revealed that all isolates were naturally sensitive to most antibiotics. The L. monocytogenes isolates had pathogenic potential. There is a potential infection risk for people who come to frequent contact with black-headed gulls in Kunming. Hence, it is necessary to conduct surveillance of L. monocytogenes in the migratory black-headed gulls, which would be valuable to prevent listeriosis in local residents and tourists. Keywords: Listeria monocytogenes, Virulence gene, PFGE, MLST, Antibiotic resistance |
url |
http://www.sciencedirect.com/science/article/pii/S1876034117300953 |
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