Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine Production

Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine Production

(1) Background: Physical stimuli may activate peripheral blood mononuclear cells (PBMCs) to secrete cytokines, which may favor pro-inflammatory responses or trigger reparative phenomena. The purpose of this study is to evaluate the action of Polarized Polychromatic Incoherent Low Energy Radiation (P...

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Main Authors: Francesca Maria Salmeri, Lucia Denaro, Elisa Ruello, Giuseppe Acri, Sergio Gurgone, Carlo Sansotta, Barbara Testagrossa
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:International Journal of Environmental Research and Public Health
Subjects:
piler
pbmcs
cytokines
Online Access:https://www.mdpi.com/1660-4601/17/4/1233
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spelling doaj-a9a82b1b9057498fb2a418c05138d4d72020-11-25T02:06:04ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012020-02-01174123310.3390/ijerph17041233ijerph17041233Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine ProductionFrancesca Maria Salmeri0Lucia Denaro1Elisa Ruello2Giuseppe Acri3Sergio Gurgone4Carlo Sansotta5Barbara Testagrossa6Department of BIOMORF, University of Messina, Via Consolare Valeria, 98125 Messina, ItalyDepartment of BIOMORF, University of Messina, Via Consolare Valeria, 98125 Messina, ItalyDepartment of BIOMORF, University of Messina, Via Consolare Valeria, 98125 Messina, ItalyDepartment of BIOMORF, University of Messina, Via Consolare Valeria, 98125 Messina, ItalyDepartment of MIFT, University of Messina, Viale F. Stagno d’Alcontres, 98166 Messina, ItalyDepartment of BIOMORF, University of Messina, Via Consolare Valeria, 98125 Messina, ItalyDepartment of BIOMORF, University of Messina, Via Consolare Valeria, 98125 Messina, Italy(1) Background: Physical stimuli may activate peripheral blood mononuclear cells (PBMCs) to secrete cytokines, which may favor pro-inflammatory responses or trigger reparative phenomena. The purpose of this study is to evaluate the action of Polarized Polychromatic Incoherent Low Energy Radiation (PILER) on human in vitro PBMCs, by detection of the possible effects on cytokine production; (2) Methods: isolated PBMCs were irradiated with a PILER lamp at different exposure times, at a distance of 10 cm, before incubation. The supernatants were collected after 24 h and 48 h and cytokines evaluated by ELISA; (3) Results: Our results showed a decrease in the levels of pro-inflammatory IL-12p70, IL-17A, IFN-γ, and TNF-α cytokines, whereas IL-10 and TGF-β1 with regulatory activity increased; (4) Conclusions: PILER irradiation affected the cytokine production by isolated PBMCs driving the immune response toward an anti-inflammatory/reparative profile.https://www.mdpi.com/1660-4601/17/4/1233pilerpbmcscytokines
collection DOAJ
language English
format Article
sources DOAJ
author Francesca Maria Salmeri
Lucia Denaro
Elisa Ruello
Giuseppe Acri
Sergio Gurgone
Carlo Sansotta
Barbara Testagrossa
spellingShingle Francesca Maria Salmeri
Lucia Denaro
Elisa Ruello
Giuseppe Acri
Sergio Gurgone
Carlo Sansotta
Barbara Testagrossa
Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine Production
International Journal of Environmental Research and Public Health
piler
pbmcs
cytokines
author_facet Francesca Maria Salmeri
Lucia Denaro
Elisa Ruello
Giuseppe Acri
Sergio Gurgone
Carlo Sansotta
Barbara Testagrossa
author_sort Francesca Maria Salmeri
title Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine Production
title_short Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine Production
title_full Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine Production
title_fullStr Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine Production
title_full_unstemmed Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine Production
title_sort irradiation with polychromatic incoherent low energy radiation of human peripheral blood mononuclear cells in vitro: effects on cytokine production
publisher MDPI AG
series International Journal of Environmental Research and Public Health
issn 1660-4601
publishDate 2020-02-01
description (1) Background: Physical stimuli may activate peripheral blood mononuclear cells (PBMCs) to secrete cytokines, which may favor pro-inflammatory responses or trigger reparative phenomena. The purpose of this study is to evaluate the action of Polarized Polychromatic Incoherent Low Energy Radiation (PILER) on human in vitro PBMCs, by detection of the possible effects on cytokine production; (2) Methods: isolated PBMCs were irradiated with a PILER lamp at different exposure times, at a distance of 10 cm, before incubation. The supernatants were collected after 24 h and 48 h and cytokines evaluated by ELISA; (3) Results: Our results showed a decrease in the levels of pro-inflammatory IL-12p70, IL-17A, IFN-γ, and TNF-α cytokines, whereas IL-10 and TGF-β1 with regulatory activity increased; (4) Conclusions: PILER irradiation affected the cytokine production by isolated PBMCs driving the immune response toward an anti-inflammatory/reparative profile.
topic piler
pbmcs
cytokines
url https://www.mdpi.com/1660-4601/17/4/1233
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