An In Vitro Mixed Infection Model With Commensal and Pathogenic Staphylococci for the Exploration of Interspecific Interactions and Their Impacts on Skin Physiology
The skin microbiota has been recognized to play an integral role in the physiology and pathology of the skin. The crosstalk between skin and the resident microbes has been extensively investigated using two-dimensional (2D) and three-dimensional (3D) cell cultures in vitro; however, skin colonizatio...
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doaj-a9a8f8525cd6493db2be069f604aa9612021-09-16T05:17:21ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-09-011110.3389/fcimb.2021.712360712360An In Vitro Mixed Infection Model With Commensal and Pathogenic Staphylococci for the Exploration of Interspecific Interactions and Their Impacts on Skin PhysiologyKatsunori Kohda0Xuan Li1Naoki Soga2Risa Nagura3Tie Duerna4Saeko Nakajima5Ichiro Nakagawa6Masakazu Ito7Akinori Ikeuchi8Frontier Research Center, Toyota Motor Corporation, Toyota, JapanFrontier Research Center, Toyota Motor Corporation, Toyota, JapanFrontier Research Center, Toyota Motor Corporation, Toyota, JapanFrontier Research Center, Toyota Motor Corporation, Toyota, JapanDepartment of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, JapanDepartment of Microbiology, Kyoto University Graduate School of Medicine, Kyoto, JapanFrontier Research Center, Toyota Motor Corporation, Toyota, JapanFrontier Research Center, Toyota Motor Corporation, Toyota, JapanThe skin microbiota has been recognized to play an integral role in the physiology and pathology of the skin. The crosstalk between skin and the resident microbes has been extensively investigated using two-dimensional (2D) and three-dimensional (3D) cell cultures in vitro; however, skin colonization by multiple species and the effects of interspecific interactions on the structure and function of skin remains to be elucidated. This study reports the establishment of a mixed infection model, incorporating both commensal (Staphylococcus epidermidis) and pathogenic (Staphylococcus aureus) bacteria, based on a 3D human epidermal model. We observed that co-infecting the 3D epidermal model with S. aureus and S. epidermidis restricted the growth of S. aureus. In addition, S. aureus induced epidermal cytotoxicity, and the release of proinflammatory cytokines was attenuated by the S. aureus-S. epidermidis mixed infection model. S. epidermidis also inhibited the invasion of the deeper epidermis by S. aureus, eliciting protective effects on the integrity of the epidermal barrier. This 3D culture-based mixed infection model would be an effective replacement for existing animal models and 2D cell culture approaches for the evaluation of diverse biotic and abiotic factors involved in maintaining skin health.https://www.frontiersin.org/articles/10.3389/fcimb.2021.712360/fullskin microbiota3D human epidermal equivalentStaphylococcus aureusStaphylococcus epidermidiscytotoxicityskin inflammation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katsunori Kohda Xuan Li Naoki Soga Risa Nagura Tie Duerna Saeko Nakajima Ichiro Nakagawa Masakazu Ito Akinori Ikeuchi |
spellingShingle |
Katsunori Kohda Xuan Li Naoki Soga Risa Nagura Tie Duerna Saeko Nakajima Ichiro Nakagawa Masakazu Ito Akinori Ikeuchi An In Vitro Mixed Infection Model With Commensal and Pathogenic Staphylococci for the Exploration of Interspecific Interactions and Their Impacts on Skin Physiology Frontiers in Cellular and Infection Microbiology skin microbiota 3D human epidermal equivalent Staphylococcus aureus Staphylococcus epidermidis cytotoxicity skin inflammation |
author_facet |
Katsunori Kohda Xuan Li Naoki Soga Risa Nagura Tie Duerna Saeko Nakajima Ichiro Nakagawa Masakazu Ito Akinori Ikeuchi |
author_sort |
Katsunori Kohda |
title |
An In Vitro Mixed Infection Model With Commensal and Pathogenic Staphylococci for the Exploration of Interspecific Interactions and Their Impacts on Skin Physiology |
title_short |
An In Vitro Mixed Infection Model With Commensal and Pathogenic Staphylococci for the Exploration of Interspecific Interactions and Their Impacts on Skin Physiology |
title_full |
An In Vitro Mixed Infection Model With Commensal and Pathogenic Staphylococci for the Exploration of Interspecific Interactions and Their Impacts on Skin Physiology |
title_fullStr |
An In Vitro Mixed Infection Model With Commensal and Pathogenic Staphylococci for the Exploration of Interspecific Interactions and Their Impacts on Skin Physiology |
title_full_unstemmed |
An In Vitro Mixed Infection Model With Commensal and Pathogenic Staphylococci for the Exploration of Interspecific Interactions and Their Impacts on Skin Physiology |
title_sort |
in vitro mixed infection model with commensal and pathogenic staphylococci for the exploration of interspecific interactions and their impacts on skin physiology |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2021-09-01 |
description |
The skin microbiota has been recognized to play an integral role in the physiology and pathology of the skin. The crosstalk between skin and the resident microbes has been extensively investigated using two-dimensional (2D) and three-dimensional (3D) cell cultures in vitro; however, skin colonization by multiple species and the effects of interspecific interactions on the structure and function of skin remains to be elucidated. This study reports the establishment of a mixed infection model, incorporating both commensal (Staphylococcus epidermidis) and pathogenic (Staphylococcus aureus) bacteria, based on a 3D human epidermal model. We observed that co-infecting the 3D epidermal model with S. aureus and S. epidermidis restricted the growth of S. aureus. In addition, S. aureus induced epidermal cytotoxicity, and the release of proinflammatory cytokines was attenuated by the S. aureus-S. epidermidis mixed infection model. S. epidermidis also inhibited the invasion of the deeper epidermis by S. aureus, eliciting protective effects on the integrity of the epidermal barrier. This 3D culture-based mixed infection model would be an effective replacement for existing animal models and 2D cell culture approaches for the evaluation of diverse biotic and abiotic factors involved in maintaining skin health. |
topic |
skin microbiota 3D human epidermal equivalent Staphylococcus aureus Staphylococcus epidermidis cytotoxicity skin inflammation |
url |
https://www.frontiersin.org/articles/10.3389/fcimb.2021.712360/full |
work_keys_str_mv |
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