Development of an immunocolloidal strip for rapid detection of picoxystrobin

In this study, we developed a monoclonal antibody against picoxystrobin (PIC). After cell fusion, four cell lines (3C2, 4D1, 4G4 and 5D4) capable of producing monoclonal antibodies against oxystrobin were obtained. The monoclonal antibody produced by the cell line 3C2 was the most sensitive to PIC,...

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Main Authors:	Shanshan Song, Ziwen Chen, Jie Liu, Liguang Xu, Hua Kuang, Jianping Zhu, Liqiang Liu
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2020-01-01
Series:	Food and Agricultural Immunology
Subjects:	picoxystrobin ic-elisa monoclonal antibody immunochromatographic strips (ics)
Online Access:	http://dx.doi.org/10.1080/09540105.2020.1754344

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spelling	doaj-a9bd6407f7144c9ab7be48ac89d6f6af2020-12-17T12:50:42ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432020-01-0131171172210.1080/09540105.2020.17543441754344Development of an immunocolloidal strip for rapid detection of picoxystrobinShanshan Song0Ziwen Chen1Jie Liu2Liguang Xu3Hua Kuang4Jianping Zhu5Liqiang Liu6State Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityIn this study, we developed a monoclonal antibody against picoxystrobin (PIC). After cell fusion, four cell lines (3C2, 4D1, 4G4 and 5D4) capable of producing monoclonal antibodies against oxystrobin were obtained. The monoclonal antibody produced by the cell line 3C2 was the most sensitive to PIC, and its IC50 value was 0.1 ng/ml. The monoclonal antibody was then used to prepare immunochromatographic strips, and its cut-off values in both phosphate-buffered saline solution and cucumber samples were 50 and 100 ng/ml, respectively. It was concluded that the ICS can sensitively and rapidly detect PIC in cucumber samples.http://dx.doi.org/10.1080/09540105.2020.1754344picoxystrobinic-elisamonoclonal antibodyimmunochromatographic strips (ics)
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Shanshan Song Ziwen Chen Jie Liu Liguang Xu Hua Kuang Jianping Zhu Liqiang Liu
spellingShingle	Shanshan Song Ziwen Chen Jie Liu Liguang Xu Hua Kuang Jianping Zhu Liqiang Liu Development of an immunocolloidal strip for rapid detection of picoxystrobin Food and Agricultural Immunology picoxystrobin ic-elisa monoclonal antibody immunochromatographic strips (ics)
author_facet	Shanshan Song Ziwen Chen Jie Liu Liguang Xu Hua Kuang Jianping Zhu Liqiang Liu
author_sort	Shanshan Song
title	Development of an immunocolloidal strip for rapid detection of picoxystrobin
title_short	Development of an immunocolloidal strip for rapid detection of picoxystrobin
title_full	Development of an immunocolloidal strip for rapid detection of picoxystrobin
title_fullStr	Development of an immunocolloidal strip for rapid detection of picoxystrobin
title_full_unstemmed	Development of an immunocolloidal strip for rapid detection of picoxystrobin
title_sort	development of an immunocolloidal strip for rapid detection of picoxystrobin
publisher	Taylor & Francis Group
series	Food and Agricultural Immunology
issn	0954-0105 1465-3443
publishDate	2020-01-01
description	In this study, we developed a monoclonal antibody against picoxystrobin (PIC). After cell fusion, four cell lines (3C2, 4D1, 4G4 and 5D4) capable of producing monoclonal antibodies against oxystrobin were obtained. The monoclonal antibody produced by the cell line 3C2 was the most sensitive to PIC, and its IC50 value was 0.1 ng/ml. The monoclonal antibody was then used to prepare immunochromatographic strips, and its cut-off values in both phosphate-buffered saline solution and cucumber samples were 50 and 100 ng/ml, respectively. It was concluded that the ICS can sensitively and rapidly detect PIC in cucumber samples.
topic	picoxystrobin ic-elisa monoclonal antibody immunochromatographic strips (ics)
url	http://dx.doi.org/10.1080/09540105.2020.1754344
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Development of an immunocolloidal strip for rapid detection of picoxystrobin

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