Micropropagation of Cryptolepis buchanani Roem. & Schult.

• Main Authors: P. J. N. Prasad, T. Chakradhar, T. Pullaiah
• Format: Article
• Language: English
• Published: National Taiwan University 2004-03-01
• Series: Taiwania
• Subjects: Cryptolepis buchanani; Micropropagation; Acclimatization
• Online Access: http://tai2.ntu.edu.tw/taiwania/abstract.php?type=abstract&id=16

vitro regeneration protocol was developed for multiplication of Cryptolepis buchanani by using shoot tip, cotyledonary node and nodal explants derived from seedlings grown in vitro. The best response was achieved with nodal explants. Cultures were established placing the nodal explants on Murashige and Skoog’s (MS; 1962) medium supplemented with various cytokinins singly or in combination with auxin and gibberellin. Of the various cytokinins used singly or in combination with auxins, 6-Benzyl aminopurine (BAP) was found to be most effective for shoot proliferation. The maximum number of shoots (12.5 to 13.0 shoots / explant with a shoot length of 4.5 to 5.0 cm) were produced on MS medium fortified with BAP 2mg/L, Kinetin (KN) 0.1mg/L, Napthalene acetic acid (NAA) 0.05 mg/L and gibberellic acid (GA3) 0.05 mg/L with 60% response. Individual shoots (grown on shoot proliferation medium) were rooted on MS medium supplemented with various auxins－Indole acetic acid (IAA), Indole butyric acid (IBA), NAA－singly or in combination. Of these IBA 1 mg/L resulted in higher number of microshoots (80%) to form roots (about 6.5 to 7.0 roots / shoot and the root length of 4.0 to 4.5 cm). The in vitro raised plantlets were acclimatized successfully to pots containing a mixture of autoclaved peatmoss and compost in 1:1 ratio.