Molecular detection and antibiogram of Salmonella spp. from apparently healthy Japanese quails of three different quail farms in Mymensingh
Objective: The present study was carried out for the isolation, identification and antibiogram study of Salmonella spp. from apparently healthy Japanese quails (Coturnix japonica) at three different quail farms in Mymensingh, Bangladesh. Materials and methods: A total of 75 cloacal swab samples wer...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Network for the Veterinarians of Bangladesh
2018-03-01
|
Series: | Journal of Advanced Veterinary and Animal Research |
Subjects: | |
Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=290798 |
id |
doaj-a9e643d600be45ea81be8687e5af490e |
---|---|
record_format |
Article |
spelling |
doaj-a9e643d600be45ea81be8687e5af490e2020-11-24T23:42:46ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102018-03-0151606610.5455/javar.2018.e248290798Molecular detection and antibiogram of Salmonella spp. from apparently healthy Japanese quails of three different quail farms in MymensinghShamina Jahan0Md Asief Hossain Zihadi1KHM Nazmul Hussain Nazir2Md. Shafiqul Islam3Md. Bahanur Rahman4Marzia Rahman5Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.Objective: The present study was carried out for the isolation, identification and antibiogram study of Salmonella spp. from apparently healthy Japanese quails (Coturnix japonica) at three different quail farms in Mymensingh, Bangladesh. Materials and methods: A total of 75 cloacal swab samples were randomly collected from apparently healthy Japanese quails from three different farms at Mymensingh, Bangladesh. The samples were subjected to a series of cultural and biochemical examination for the isolation of Salmonella followed by molecular detection by polymerase chain reaction (PCR). Motility of the Salmonella was performed by motility test and amplification of speF gene. The antibiogram profile of the isolates was also evaluated against commonly used antimicrobials by disc diffusion method. Results: The overall prevalence of Salmonella spp. in quails was found to be 13.33%(n=10/75). Out of the 10 isolates, seven were found to be motile. Farmwise, the prevalence of Salmonella spp. were 10%(n=3/30), 24%(n=6/25) and 5%(n=1/20) at the quail farms of Bangladesh Agricultural University (BAU), Shikarikanda and Akua, respectively. The antibiogram study revealed that all the isolates were resistant to both Erythromycin and Tetracycline. On the other hand, 100% isolates were sensitive to both Ciprofloxacin and Imipenem. Ninety percent isolates of Salmonella were resistant to Colistin sulphate. Neomycin was found to be sensitive to 80% Salmonella isolates. All the Salmonella isolates were found to be multidrug resistant (MDR). Conclusion: The presence of MDR Salmonella spp. in quails signifies public health importance of the organisms, which may be associated with food-borne illness. [J Adv Vet Anim Res 2018; 5(1.000): 60-66]http://www.ejmanager.com/fulltextpdf.php?mno=290798AntibiogramJapanese quailMultidrug resistantPrevalencespeF gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shamina Jahan Md Asief Hossain Zihadi KHM Nazmul Hussain Nazir Md. Shafiqul Islam Md. Bahanur Rahman Marzia Rahman |
spellingShingle |
Shamina Jahan Md Asief Hossain Zihadi KHM Nazmul Hussain Nazir Md. Shafiqul Islam Md. Bahanur Rahman Marzia Rahman Molecular detection and antibiogram of Salmonella spp. from apparently healthy Japanese quails of three different quail farms in Mymensingh Journal of Advanced Veterinary and Animal Research Antibiogram Japanese quail Multidrug resistant Prevalence speF gene |
author_facet |
Shamina Jahan Md Asief Hossain Zihadi KHM Nazmul Hussain Nazir Md. Shafiqul Islam Md. Bahanur Rahman Marzia Rahman |
author_sort |
Shamina Jahan |
title |
Molecular detection and antibiogram of Salmonella spp. from apparently healthy Japanese quails of three different quail farms in Mymensingh |
title_short |
Molecular detection and antibiogram of Salmonella spp. from apparently healthy Japanese quails of three different quail farms in Mymensingh |
title_full |
Molecular detection and antibiogram of Salmonella spp. from apparently healthy Japanese quails of three different quail farms in Mymensingh |
title_fullStr |
Molecular detection and antibiogram of Salmonella spp. from apparently healthy Japanese quails of three different quail farms in Mymensingh |
title_full_unstemmed |
Molecular detection and antibiogram of Salmonella spp. from apparently healthy Japanese quails of three different quail farms in Mymensingh |
title_sort |
molecular detection and antibiogram of salmonella spp. from apparently healthy japanese quails of three different quail farms in mymensingh |
publisher |
Network for the Veterinarians of Bangladesh |
series |
Journal of Advanced Veterinary and Animal Research |
issn |
2311-7710 |
publishDate |
2018-03-01 |
description |
Objective: The present study was carried out for the isolation, identification and antibiogram study of Salmonella spp. from apparently healthy Japanese quails (Coturnix japonica) at three different quail farms in Mymensingh, Bangladesh.
Materials and methods: A total of 75 cloacal swab samples were randomly collected from apparently healthy Japanese quails from three different farms at Mymensingh, Bangladesh. The samples were subjected to a series of cultural and biochemical examination for the isolation of Salmonella followed by molecular detection by polymerase chain reaction (PCR). Motility of the Salmonella was performed by motility test and amplification of speF gene. The antibiogram profile of the isolates was also evaluated against commonly used antimicrobials by disc diffusion method.
Results: The overall prevalence of Salmonella spp. in quails was found to be 13.33%(n=10/75). Out of the 10 isolates, seven were found to be motile. Farmwise, the prevalence of Salmonella spp. were 10%(n=3/30), 24%(n=6/25) and 5%(n=1/20) at the quail farms of Bangladesh Agricultural University (BAU), Shikarikanda and Akua, respectively. The antibiogram study revealed that all the isolates were resistant to both Erythromycin and Tetracycline. On the other hand, 100% isolates were sensitive to both Ciprofloxacin and Imipenem. Ninety percent isolates of Salmonella were resistant to Colistin sulphate. Neomycin was found to be sensitive to 80% Salmonella isolates. All the Salmonella isolates were found to be multidrug resistant (MDR).
Conclusion: The presence of MDR Salmonella spp. in quails signifies public health importance of the organisms, which may be associated with food-borne illness. [J Adv Vet Anim Res 2018; 5(1.000): 60-66] |
topic |
Antibiogram Japanese quail Multidrug resistant Prevalence speF gene |
url |
http://www.ejmanager.com/fulltextpdf.php?mno=290798 |
work_keys_str_mv |
AT shaminajahan moleculardetectionandantibiogramofsalmonellasppfromapparentlyhealthyjapanesequailsofthreedifferentquailfarmsinmymensingh AT mdasiefhossainzihadi moleculardetectionandantibiogramofsalmonellasppfromapparentlyhealthyjapanesequailsofthreedifferentquailfarmsinmymensingh AT khmnazmulhussainnazir moleculardetectionandantibiogramofsalmonellasppfromapparentlyhealthyjapanesequailsofthreedifferentquailfarmsinmymensingh AT mdshafiqulislam moleculardetectionandantibiogramofsalmonellasppfromapparentlyhealthyjapanesequailsofthreedifferentquailfarmsinmymensingh AT mdbahanurrahman moleculardetectionandantibiogramofsalmonellasppfromapparentlyhealthyjapanesequailsofthreedifferentquailfarmsinmymensingh AT marziarahman moleculardetectionandantibiogramofsalmonellasppfromapparentlyhealthyjapanesequailsofthreedifferentquailfarmsinmymensingh |
_version_ |
1725503035028799488 |