CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation...

Full description

Bibliographic Details
Main Authors:	Yan ZHANG, Sachula WU, Fen-hua LUO, Baiyinbatu, Lin-hong LIU, Tian-yuan HU, Boyang YU, Guang-peng LI, Ying-ji WU
Format:	Article
Language:	English
Published:	Elsevier 2014-08-01
Series:	Journal of Integrative Agriculture
Subjects:	sheep spermatogonial stem cells CDH1 polyclonal antibodies immunofluorescent staining
Online Access:	http://www.sciencedirect.com/science/article/pii/S2095311913606899

id	doaj-a9e8cb10ffdb46f9a2d8d489b5643ebb
record_format	Article
spelling	doaj-a9e8cb10ffdb46f9a2d8d489b5643ebb2021-06-07T06:49:48ZengElsevierJournal of Integrative Agriculture2095-31192014-08-0113817591765CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep TestisYan ZHANG0Sachula WU1Fen-hua LUO2 Baiyinbatu3Lin-hong LIU4Tian-yuan HU5Boyang YU6Guang-peng LI7Ying-ji WU8The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China; School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010021, P.R.ChinaThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.ChinaThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.ChinaThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.ChinaThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.ChinaThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.ChinaThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.ChinaThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.ChinaThe Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, P.R.China; Correspondence WU Ying-ji, Tel: +86-471-4992443, Mobile: 18686031623Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1 (cadherin-1, also known as E-cadherin) can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep anti-CDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.http://www.sciencedirect.com/science/article/pii/S2095311913606899sheepspermatogonial stem cellsCDH1polyclonal antibodiesimmunofluorescent staining
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yan ZHANG Sachula WU Fen-hua LUO Baiyinbatu Lin-hong LIU Tian-yuan HU Boyang YU Guang-peng LI Ying-ji WU
spellingShingle	Yan ZHANG Sachula WU Fen-hua LUO Baiyinbatu Lin-hong LIU Tian-yuan HU Boyang YU Guang-peng LI Ying-ji WU CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis Journal of Integrative Agriculture sheep spermatogonial stem cells CDH1 polyclonal antibodies immunofluorescent staining
author_facet	Yan ZHANG Sachula WU Fen-hua LUO Baiyinbatu Lin-hong LIU Tian-yuan HU Boyang YU Guang-peng LI Ying-ji WU
author_sort	Yan ZHANG
title	CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis
title_short	CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis
title_full	CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis
title_fullStr	CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis
title_full_unstemmed	CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis
title_sort	cdh1, a novel surface marker of spermatogonial stem cells in sheep testis
publisher	Elsevier
series	Journal of Integrative Agriculture
issn	2095-3119
publishDate	2014-08-01
description	Spermatogonial stem cells (SSCs) are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1 (cadherin-1, also known as E-cadherin) can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep anti-CDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.
topic	sheep spermatogonial stem cells CDH1 polyclonal antibodies immunofluorescent staining
url	http://www.sciencedirect.com/science/article/pii/S2095311913606899
work_keys_str_mv	AT yanzhang cdh1anovelsurfacemarkerofspermatogonialstemcellsinsheeptestis AT sachulawu cdh1anovelsurfacemarkerofspermatogonialstemcellsinsheeptestis AT fenhualuo cdh1anovelsurfacemarkerofspermatogonialstemcellsinsheeptestis AT baiyinbatu cdh1anovelsurfacemarkerofspermatogonialstemcellsinsheeptestis AT linhongliu cdh1anovelsurfacemarkerofspermatogonialstemcellsinsheeptestis AT tianyuanhu cdh1anovelsurfacemarkerofspermatogonialstemcellsinsheeptestis AT boyangyu cdh1anovelsurfacemarkerofspermatogonialstemcellsinsheeptestis AT guangpengli cdh1anovelsurfacemarkerofspermatogonialstemcellsinsheeptestis AT yingjiwu cdh1anovelsurfacemarkerofspermatogonialstemcellsinsheeptestis
_version_	1721392431244509184

CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

Similar Items