Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells
This report describes our initial attempt to regenerate salivary glands using induced pluripotent stem (iPS) cells in vivo and in vitro. Glandular tissues that were similar to the adult submandibular glands (SMGs) and sublingual glands could be partially produced by the transplantation of iPS cells...
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Online Access: | http://dx.doi.org/10.1155/2015/293570 |
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doaj-a9e94d01b28d41a1bcaaa055a1c45ccc2020-11-24T22:56:57ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/293570293570Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem CellsHitomi Ono0Aya Obana1Yu Usami2Manabu Sakai3Kanji Nohara4Hiroshi Egusa5Takayoshi Sakai6Department of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, JapanDepartment of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, JapanDivision of Orthopedic Surgery, The Children’s Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USAClinical Laboratory, Osaka University Dental Hospital, Suita, Osaka 565-0871, JapanDepartment of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, JapanDivision of Molecular and Regenerative Prosthodontics, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi 980-8575, JapanDepartment of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, JapanThis report describes our initial attempt to regenerate salivary glands using induced pluripotent stem (iPS) cells in vivo and in vitro. Glandular tissues that were similar to the adult submandibular glands (SMGs) and sublingual glands could be partially produced by the transplantation of iPS cells into mouse salivary glands. However, the tumorigenicity of iPS cells has not been resolved yet. It is well known that stem cells affect their microenvironment, known as a stem cell niche. We focused on the niche and the interaction between iPS cells and salivary gland cells in our study on salivary gland regeneration. Coculture of embryonic SMG cells and iPS cells have better-developed epithelial structures and fewer undifferentiated specific markers than monoculture of embryonic SMG cells in vitro. These results suggest that iPS cells have a potential ability to accelerate differentiation for salivary gland development and regeneration.http://dx.doi.org/10.1155/2015/293570 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hitomi Ono Aya Obana Yu Usami Manabu Sakai Kanji Nohara Hiroshi Egusa Takayoshi Sakai |
spellingShingle |
Hitomi Ono Aya Obana Yu Usami Manabu Sakai Kanji Nohara Hiroshi Egusa Takayoshi Sakai Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells BioMed Research International |
author_facet |
Hitomi Ono Aya Obana Yu Usami Manabu Sakai Kanji Nohara Hiroshi Egusa Takayoshi Sakai |
author_sort |
Hitomi Ono |
title |
Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells |
title_short |
Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells |
title_full |
Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells |
title_fullStr |
Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells |
title_full_unstemmed |
Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells |
title_sort |
regenerating salivary glands in the microenvironment of induced pluripotent stem cells |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
This report describes our initial attempt to regenerate salivary glands using induced pluripotent stem (iPS) cells in vivo and in vitro. Glandular tissues that were similar to the adult submandibular glands (SMGs) and sublingual glands could be partially produced by the transplantation of iPS cells into mouse salivary glands. However, the tumorigenicity of iPS cells has not been resolved yet. It is well known that stem cells affect their microenvironment, known as a stem cell niche. We focused on the niche and the interaction between iPS cells and salivary gland cells in our study on salivary gland regeneration. Coculture of embryonic SMG cells and iPS cells have better-developed epithelial structures and fewer undifferentiated specific markers than monoculture of embryonic SMG cells in vitro. These results suggest that iPS cells have a potential ability to accelerate differentiation for salivary gland development and regeneration. |
url |
http://dx.doi.org/10.1155/2015/293570 |
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