Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil

Extra virgin olive oil (EVOO) phenols represent a significant part of the intake of antioxidants and bioactive compounds in the Mediterranean diet. In particular, hydroxytyrosol (HTyr), tyrosol (Tyr), and the secoiridoids oleacein and oleocanthal play central roles as anti-inflammatory, neuro-protec...

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Main Authors:	Antonio Francioso, Rodolfo Federico, Anna Maggiore, Mario Fontana, Alberto Boffi, Maria D’Erme, Luciana Mosca
Format:	Article
Language:	English
Published:	MDPI AG 2020-08-01
Series:	Molecules
Subjects:	extra virgin olive oil oleocanthal secoiridoids green chemistry hydoxytyrosol tyrosol
Online Access:	https://www.mdpi.com/1420-3049/25/16/3654

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spelling	doaj-a9ee44452a7b43d2a62a946142a0a66c2020-11-25T03:07:26ZengMDPI AGMolecules1420-30492020-08-01253654365410.3390/molecules25163654Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive OilAntonio Francioso0Rodolfo Federico1Anna Maggiore2Mario Fontana3Alberto Boffi4Maria D’Erme5Luciana Mosca6Department of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyMOLIROM s.r.l, via Carlo Bartolomeo Piazza 8, 00161 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyExtra virgin olive oil (EVOO) phenols represent a significant part of the intake of antioxidants and bioactive compounds in the Mediterranean diet. In particular, hydroxytyrosol (HTyr), tyrosol (Tyr), and the secoiridoids oleacein and oleocanthal play central roles as anti-inflammatory, neuro-protective and anti-cancer agents. These compounds cannot be easily obtained via chemical synthesis, and their isolation and purification from EVOO is cumbersome. Indeed, both processes involve the use of large volumes of organic solvents, hazardous reagents and several chromatographic steps. In this work we propose a novel optimized procedure for the green extraction, isolation and purification of HTyr, Tyr, oleacein and oleocanthal directly from EVOO, by using a Natural Deep Eutectic Solvent (NaDES) as an extracting phase, coupled with preparative high-performance liquid chromatography. This purification method allows the total recovery of the four components as single pure compounds directly from EVOO, in a rapid, economic and ecologically sustainable way, which utilizes biocompatible reagents and strongly limits the use or generation of hazardous substances.https://www.mdpi.com/1420-3049/25/16/3654extra virgin olive oiloleocanthalsecoiridoidsgreen chemistryhydoxytyrosoltyrosol
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Antonio Francioso Rodolfo Federico Anna Maggiore Mario Fontana Alberto Boffi Maria D’Erme Luciana Mosca
spellingShingle	Antonio Francioso Rodolfo Federico Anna Maggiore Mario Fontana Alberto Boffi Maria D’Erme Luciana Mosca Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil Molecules extra virgin olive oil oleocanthal secoiridoids green chemistry hydoxytyrosol tyrosol
author_facet	Antonio Francioso Rodolfo Federico Anna Maggiore Mario Fontana Alberto Boffi Maria D’Erme Luciana Mosca
author_sort	Antonio Francioso
title	Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil
title_short	Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil
title_full	Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil
title_fullStr	Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil
title_full_unstemmed	Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil
title_sort	green route for the isolation and purification of hyrdoxytyrosol, tyrosol, oleacein and oleocanthal from extra virgin olive oil
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2020-08-01
description	Extra virgin olive oil (EVOO) phenols represent a significant part of the intake of antioxidants and bioactive compounds in the Mediterranean diet. In particular, hydroxytyrosol (HTyr), tyrosol (Tyr), and the secoiridoids oleacein and oleocanthal play central roles as anti-inflammatory, neuro-protective and anti-cancer agents. These compounds cannot be easily obtained via chemical synthesis, and their isolation and purification from EVOO is cumbersome. Indeed, both processes involve the use of large volumes of organic solvents, hazardous reagents and several chromatographic steps. In this work we propose a novel optimized procedure for the green extraction, isolation and purification of HTyr, Tyr, oleacein and oleocanthal directly from EVOO, by using a Natural Deep Eutectic Solvent (NaDES) as an extracting phase, coupled with preparative high-performance liquid chromatography. This purification method allows the total recovery of the four components as single pure compounds directly from EVOO, in a rapid, economic and ecologically sustainable way, which utilizes biocompatible reagents and strongly limits the use or generation of hazardous substances.
topic	extra virgin olive oil oleocanthal secoiridoids green chemistry hydoxytyrosol tyrosol
url	https://www.mdpi.com/1420-3049/25/16/3654
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Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil

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