Specificity and selectivity improvement in doping analysis using comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry

• Main Authors: Samantha S. Barbosa, Felipe D. Leal, Monica C. Padilha, Raphael S. F. Silva, Henrique Marcelo G. Pereira, Francisco R. Aquino Neto, Ademário I. da Silva Júnior
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2012-01-01
• Series: Química Nova
• Subjects: comprehensive two-dimensional gas chromatography; doping analysis; time-of-flight
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000500022&lng=en&tlng=en
• ISSN: 1678-7064

Comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry was used for the identification of forty doping agents. The improvement in the specificity was remarkable, allowing the resolution of analytes that could not be done by one-dimensional chromatographic systems. The sensitivity observed for different classes of prohibited substances was clearly below the value required by the World Anti-Doping Agency. In addition time-of-flight mass spectrometry gives full spectrum for all analytes without any interference from the matrix, resulting in selectivity improvements. These results could support the implementation of an exhaustive monitoring approach for hundreds of doping agents in a single injection.