Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells
The aim of the study was to test and compare the cytotoxic potential of two synthetic oestrogens: diethylstilboestrol (DES) and ethinyloestradiol (EE2) and two androgens: testosterone propionate (TP) and trenbolone (TREN) on two cell lines. The fibroblast cell line Balb/c 3T3 and the hepatoma cell l...
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doaj-aa4d6aad72e345dc99a3a466c01b78bb2021-09-22T06:13:24ZengSciendoBulletin of the Veterinary Institute in Pulawy2300-32352014-12-0158461362010.2478/bvip-2014-0094bvip-2014-0094Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cellsMinta Maria0Radko Lidia1Stypuła-Trębas Sylwia2Żmudzki Jan3Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100Pulawy, PolandDepartment of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100Pulawy, PolandDepartment of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100Pulawy, PolandDepartment of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100Pulawy, PolandThe aim of the study was to test and compare the cytotoxic potential of two synthetic oestrogens: diethylstilboestrol (DES) and ethinyloestradiol (EE2) and two androgens: testosterone propionate (TP) and trenbolone (TREN) on two cell lines. The fibroblast cell line Balb/c 3T3 and the hepatoma cell line HepG2 were selected. To get more insight into the mode of toxic action, four methods were used, which evaluated different biochemical endpoints: mitochondrial activity (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide reduction assay), lysosomal activity (neutral red uptake assay), total protein content, and lactate dehydrogenase release. Cytotoxicity was assessed after 24, 48, and 72 h exposure to eight concentrations ranging from 0.78 to 100 μg/mL. Concentration- and time- dependent effects were observed. Depending on the line and assay used, half maximal effective concentration after 72 h (EC50-72h) values ranged as follows: DES 1-13.7 μg/mL (Balb/c 3T3) and 3.7-5.2 μg/mL (HepG2); EE2 2.1-14.3 μg/mL (Balb/c 3T3) and 1.8-7.8 μg/mL (HepG2); TP-14.9-17.5 μg/mL (Balb/c 3T3), and 63.9- 100 μg/mL (HepG2); and TREN 11.3-31.4 μg/mL (Balb/c 3T3) and 12.5-59.4 μg/mL (HepG2). The results revealed that oestrogens were more toxic than androgens and the most affected endpoint was mitochondrial activity. In contrast to oestrogens, for which EC50-72h values were similar in both lines and by all assays used, Balb/c 3T3 cells were more sensitive than HepG2 cells to TP.https://doi.org/10.2478/bvip-2014-0094synthetic hormonescytotoxicitybalb/c 3t3hepg2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Minta Maria Radko Lidia Stypuła-Trębas Sylwia Żmudzki Jan |
spellingShingle |
Minta Maria Radko Lidia Stypuła-Trębas Sylwia Żmudzki Jan Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells Bulletin of the Veterinary Institute in Pulawy synthetic hormones cytotoxicity balb/c 3t3 hepg2 |
author_facet |
Minta Maria Radko Lidia Stypuła-Trębas Sylwia Żmudzki Jan |
author_sort |
Minta Maria |
title |
Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells |
title_short |
Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells |
title_full |
Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells |
title_fullStr |
Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells |
title_full_unstemmed |
Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells |
title_sort |
cytotoxic effects of the synthetic oestrogens and androgens on balb/c 3t3 and hepg2 cells |
publisher |
Sciendo |
series |
Bulletin of the Veterinary Institute in Pulawy |
issn |
2300-3235 |
publishDate |
2014-12-01 |
description |
The aim of the study was to test and compare the cytotoxic potential of two synthetic oestrogens: diethylstilboestrol (DES) and ethinyloestradiol (EE2) and two androgens: testosterone propionate (TP) and trenbolone (TREN) on two cell lines. The fibroblast cell line Balb/c 3T3 and the hepatoma cell line HepG2 were selected. To get more insight into the mode of toxic action, four methods were used, which evaluated different biochemical endpoints: mitochondrial activity (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide reduction assay), lysosomal activity (neutral red uptake assay), total protein content, and lactate dehydrogenase release. Cytotoxicity was assessed after 24, 48, and 72 h exposure to eight concentrations ranging from 0.78 to 100 μg/mL. Concentration- and time- dependent effects were observed. Depending on the line and assay used, half maximal effective concentration after 72 h (EC50-72h) values ranged as follows: DES 1-13.7 μg/mL (Balb/c 3T3) and 3.7-5.2 μg/mL (HepG2); EE2 2.1-14.3 μg/mL (Balb/c 3T3) and 1.8-7.8 μg/mL (HepG2); TP-14.9-17.5 μg/mL (Balb/c 3T3), and 63.9- 100 μg/mL (HepG2); and TREN 11.3-31.4 μg/mL (Balb/c 3T3) and 12.5-59.4 μg/mL (HepG2). The results revealed that oestrogens were more toxic than androgens and the most affected endpoint was mitochondrial activity. In contrast to oestrogens, for which EC50-72h values were similar in both lines and by all assays used, Balb/c 3T3 cells were more sensitive than HepG2 cells to TP. |
topic |
synthetic hormones cytotoxicity balb/c 3t3 hepg2 |
url |
https://doi.org/10.2478/bvip-2014-0094 |
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