AFM and MFM techniques for enzyme activity imaging and quantification

In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The r...

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Main Authors:	Michelle Arredondo, Margarita Stoytcheva, Israel Morales-Reyes, Nikola Batina
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2018-07-01
Series:	Biotechnology & Biotechnological Equipment
Subjects:	Atomic force microscopy magnetic force microscopy trypsin activity ferrogel
Online Access:	http://dx.doi.org/10.1080/13102818.2018.1470904

id	doaj-aa6563ce20cf4c2dae050d903fad4ba6
record_format	Article
spelling	doaj-aa6563ce20cf4c2dae050d903fad4ba62020-11-25T00:05:42ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302018-07-013241065107410.1080/13102818.2018.14709041470904AFM and MFM techniques for enzyme activity imaging and quantificationMichelle Arredondo0Margarita Stoytcheva1Israel Morales-Reyes2Nikola Batina3Tecnológico Nacional de MéxicoUniversidad Autónoma de Baja CaliforniaDivisión de Ciencias Básicas e IngenieríaDivisión de Ciencias Básicas e IngenieríaIn this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained.http://dx.doi.org/10.1080/13102818.2018.1470904Atomic force microscopymagnetic force microscopytrypsin activityferrogel
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Michelle Arredondo Margarita Stoytcheva Israel Morales-Reyes Nikola Batina
spellingShingle	Michelle Arredondo Margarita Stoytcheva Israel Morales-Reyes Nikola Batina AFM and MFM techniques for enzyme activity imaging and quantification Biotechnology & Biotechnological Equipment Atomic force microscopy magnetic force microscopy trypsin activity ferrogel
author_facet	Michelle Arredondo Margarita Stoytcheva Israel Morales-Reyes Nikola Batina
author_sort	Michelle Arredondo
title	AFM and MFM techniques for enzyme activity imaging and quantification
title_short	AFM and MFM techniques for enzyme activity imaging and quantification
title_full	AFM and MFM techniques for enzyme activity imaging and quantification
title_fullStr	AFM and MFM techniques for enzyme activity imaging and quantification
title_full_unstemmed	AFM and MFM techniques for enzyme activity imaging and quantification
title_sort	afm and mfm techniques for enzyme activity imaging and quantification
publisher	Taylor & Francis Group
series	Biotechnology & Biotechnological Equipment
issn	1310-2818 1314-3530
publishDate	2018-07-01
description	In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained.
topic	Atomic force microscopy magnetic force microscopy trypsin activity ferrogel
url	http://dx.doi.org/10.1080/13102818.2018.1470904
work_keys_str_mv	AT michellearredondo afmandmfmtechniquesforenzymeactivityimagingandquantification AT margaritastoytcheva afmandmfmtechniquesforenzymeactivityimagingandquantification AT israelmoralesreyes afmandmfmtechniquesforenzymeactivityimagingandquantification AT nikolabatina afmandmfmtechniquesforenzymeactivityimagingandquantification
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AFM and MFM techniques for enzyme activity imaging and quantification

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