AFM and MFM techniques for enzyme activity imaging and quantification
In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The r...
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Online Access: | http://dx.doi.org/10.1080/13102818.2018.1470904 |
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doaj-aa6563ce20cf4c2dae050d903fad4ba62020-11-25T00:05:42ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302018-07-013241065107410.1080/13102818.2018.14709041470904AFM and MFM techniques for enzyme activity imaging and quantificationMichelle Arredondo0Margarita Stoytcheva1Israel Morales-Reyes2Nikola Batina3Tecnológico Nacional de MéxicoUniversidad Autónoma de Baja CaliforniaDivisión de Ciencias Básicas e IngenieríaDivisión de Ciencias Básicas e IngenieríaIn this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained.http://dx.doi.org/10.1080/13102818.2018.1470904Atomic force microscopymagnetic force microscopytrypsin activityferrogel |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michelle Arredondo Margarita Stoytcheva Israel Morales-Reyes Nikola Batina |
spellingShingle |
Michelle Arredondo Margarita Stoytcheva Israel Morales-Reyes Nikola Batina AFM and MFM techniques for enzyme activity imaging and quantification Biotechnology & Biotechnological Equipment Atomic force microscopy magnetic force microscopy trypsin activity ferrogel |
author_facet |
Michelle Arredondo Margarita Stoytcheva Israel Morales-Reyes Nikola Batina |
author_sort |
Michelle Arredondo |
title |
AFM and MFM techniques for enzyme activity imaging and quantification |
title_short |
AFM and MFM techniques for enzyme activity imaging and quantification |
title_full |
AFM and MFM techniques for enzyme activity imaging and quantification |
title_fullStr |
AFM and MFM techniques for enzyme activity imaging and quantification |
title_full_unstemmed |
AFM and MFM techniques for enzyme activity imaging and quantification |
title_sort |
afm and mfm techniques for enzyme activity imaging and quantification |
publisher |
Taylor & Francis Group |
series |
Biotechnology & Biotechnological Equipment |
issn |
1310-2818 1314-3530 |
publishDate |
2018-07-01 |
description |
In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained. |
topic |
Atomic force microscopy magnetic force microscopy trypsin activity ferrogel |
url |
http://dx.doi.org/10.1080/13102818.2018.1470904 |
work_keys_str_mv |
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_version_ |
1725423829095809024 |