A high content, phenotypic ‘scar-in-a-jar’ assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts

A high content, phenotypic ‘scar-in-a-jar’ assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts

Abstract Background Excessive extracellular matrix (ECM) deposition is a hallmark feature in fibrosis and tissue remodelling diseases. Typically, mesenchymal cells will produce collagens under standard 2D cell culture conditions, however these do not assemble into fibrils. Existing assays for measur...

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Bibliographic Details
Main Authors: Robert B. Good, Jessica D. Eley, Elaine Gower, Genevieve Butt, Andrew D. Blanchard, Andrew J. Fisher, Carmel B. Nanthakumar
Format: Article
Language:English
Published: BMC 2019-06-01
Series:BMC Biomedical Engineering
Subjects:
Scar-in-a-jar
Extracellular matrix
ECM
Fibrosis
Fibroblast
Pulmonary fibrosis
Online Access:http://link.springer.com/article/10.1186/s42490-019-0014-z

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http://link.springer.com/article/10.1186/s42490-019-0014-z

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