iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.

Autophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce a variety of metabolites. In plants, little is kno...

Full description

Bibliographic Details
Main Authors: Xiaoai Fang, Xiangyu Yao, Yue Zhang, Zheni Tian, Meng Wang, Peng Li, Xia Cai
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:International Journal of Molecular Sciences
Subjects:
ROS
Online Access:https://www.mdpi.com/1422-0067/20/15/3770
id doaj-aa8dd31e1aae4f009e2afa30f1840de3
record_format Article
spelling doaj-aa8dd31e1aae4f009e2afa30f1840de32020-11-24T21:29:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-08-012015377010.3390/ijms20153770ijms20153770iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.Xiaoai Fang0Xiangyu Yao1Yue Zhang2Zheni Tian3Meng Wang4Peng Li5Xia Cai6Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaAutophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce a variety of metabolites. In plants, little is known about autophagy-mediated responses against MeJA. In this study, we used high-throughput comparative proteomics to identify proteins of latex in the laticifers. The isobaric tags for relative and absolute quantification (iTRAQ) MS/MS proteomics were performed, and 298 proteins among MeJA treated groups and the control group of <i>Euphorbia kansui</i> were identified. It is interesting to note that 29 significant differentially expressed proteins were identified and their associations with autophagy and ROS pathway were verified for several selected proteins as follows: &#945;-L-fucosidase, &#946;-galactosidase, cysteine proteinase, and Cu/Zn superoxide dismutase. Quantitative real-time PCR analysis of the selected genes confirmed the fact that MeJA might enhance the expression of some genes related to autophagy. The western blotting and immunofluorescence results of ATG8 and ATG18a which are two important proteins for the formation of autophagosomes also demonstrated that MeJA could promote autophagy at the protein level. Using the electron microscope, we observed an increase in autophagosomes after MeJA treatment. These results indicated that MeJA might promote autophagy in <i>E. kansui</i> laticifers; and it was speculated that MeJA mediated autophagy through two possible ways: the increase of ROS induces ATG8 accumulation and then aotophagosome formation, and MeJA promotes ATG18 accumulation and then autophagosome formation. Taken together, our results provide several novel insights for understanding the mechanism between autophagy and MeJA treatment. However, the specific mechanism remains to be further studied in the future.https://www.mdpi.com/1422-0067/20/15/3770methyl jasmonateiTRAQATG8ATG18aautophagyROS<i>Euphorbia kansui</i>
collection DOAJ
language English
format Article
sources DOAJ
author Xiaoai Fang
Xiangyu Yao
Yue Zhang
Zheni Tian
Meng Wang
Peng Li
Xia Cai
spellingShingle Xiaoai Fang
Xiangyu Yao
Yue Zhang
Zheni Tian
Meng Wang
Peng Li
Xia Cai
iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.
International Journal of Molecular Sciences
methyl jasmonate
iTRAQ
ATG8
ATG18a
autophagy
ROS
<i>Euphorbia kansui</i>
author_facet Xiaoai Fang
Xiangyu Yao
Yue Zhang
Zheni Tian
Meng Wang
Peng Li
Xia Cai
author_sort Xiaoai Fang
title iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.
title_short iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.
title_full iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.
title_fullStr iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.
title_full_unstemmed iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.
title_sort itraq-based proteomics analysis of autophagy-mediated responses against meja in laticifers of <i>euphorbia kansui</i> l.
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2019-08-01
description Autophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce a variety of metabolites. In plants, little is known about autophagy-mediated responses against MeJA. In this study, we used high-throughput comparative proteomics to identify proteins of latex in the laticifers. The isobaric tags for relative and absolute quantification (iTRAQ) MS/MS proteomics were performed, and 298 proteins among MeJA treated groups and the control group of <i>Euphorbia kansui</i> were identified. It is interesting to note that 29 significant differentially expressed proteins were identified and their associations with autophagy and ROS pathway were verified for several selected proteins as follows: &#945;-L-fucosidase, &#946;-galactosidase, cysteine proteinase, and Cu/Zn superoxide dismutase. Quantitative real-time PCR analysis of the selected genes confirmed the fact that MeJA might enhance the expression of some genes related to autophagy. The western blotting and immunofluorescence results of ATG8 and ATG18a which are two important proteins for the formation of autophagosomes also demonstrated that MeJA could promote autophagy at the protein level. Using the electron microscope, we observed an increase in autophagosomes after MeJA treatment. These results indicated that MeJA might promote autophagy in <i>E. kansui</i> laticifers; and it was speculated that MeJA mediated autophagy through two possible ways: the increase of ROS induces ATG8 accumulation and then aotophagosome formation, and MeJA promotes ATG18 accumulation and then autophagosome formation. Taken together, our results provide several novel insights for understanding the mechanism between autophagy and MeJA treatment. However, the specific mechanism remains to be further studied in the future.
topic methyl jasmonate
iTRAQ
ATG8
ATG18a
autophagy
ROS
<i>Euphorbia kansui</i>
url https://www.mdpi.com/1422-0067/20/15/3770
work_keys_str_mv AT xiaoaifang itraqbasedproteomicsanalysisofautophagymediatedresponsesagainstmejainlaticifersofieuphorbiakansuiil
AT xiangyuyao itraqbasedproteomicsanalysisofautophagymediatedresponsesagainstmejainlaticifersofieuphorbiakansuiil
AT yuezhang itraqbasedproteomicsanalysisofautophagymediatedresponsesagainstmejainlaticifersofieuphorbiakansuiil
AT zhenitian itraqbasedproteomicsanalysisofautophagymediatedresponsesagainstmejainlaticifersofieuphorbiakansuiil
AT mengwang itraqbasedproteomicsanalysisofautophagymediatedresponsesagainstmejainlaticifersofieuphorbiakansuiil
AT pengli itraqbasedproteomicsanalysisofautophagymediatedresponsesagainstmejainlaticifersofieuphorbiakansuiil
AT xiacai itraqbasedproteomicsanalysisofautophagymediatedresponsesagainstmejainlaticifersofieuphorbiakansuiil
_version_ 1725966433360281600