iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.
Autophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce a variety of metabolites. In plants, little is kno...
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doaj-aa8dd31e1aae4f009e2afa30f1840de32020-11-24T21:29:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-08-012015377010.3390/ijms20153770ijms20153770iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L.Xiaoai Fang0Xiangyu Yao1Yue Zhang2Zheni Tian3Meng Wang4Peng Li5Xia Cai6Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, ChinaAutophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce a variety of metabolites. In plants, little is known about autophagy-mediated responses against MeJA. In this study, we used high-throughput comparative proteomics to identify proteins of latex in the laticifers. The isobaric tags for relative and absolute quantification (iTRAQ) MS/MS proteomics were performed, and 298 proteins among MeJA treated groups and the control group of <i>Euphorbia kansui</i> were identified. It is interesting to note that 29 significant differentially expressed proteins were identified and their associations with autophagy and ROS pathway were verified for several selected proteins as follows: α-L-fucosidase, β-galactosidase, cysteine proteinase, and Cu/Zn superoxide dismutase. Quantitative real-time PCR analysis of the selected genes confirmed the fact that MeJA might enhance the expression of some genes related to autophagy. The western blotting and immunofluorescence results of ATG8 and ATG18a which are two important proteins for the formation of autophagosomes also demonstrated that MeJA could promote autophagy at the protein level. Using the electron microscope, we observed an increase in autophagosomes after MeJA treatment. These results indicated that MeJA might promote autophagy in <i>E. kansui</i> laticifers; and it was speculated that MeJA mediated autophagy through two possible ways: the increase of ROS induces ATG8 accumulation and then aotophagosome formation, and MeJA promotes ATG18 accumulation and then autophagosome formation. Taken together, our results provide several novel insights for understanding the mechanism between autophagy and MeJA treatment. However, the specific mechanism remains to be further studied in the future.https://www.mdpi.com/1422-0067/20/15/3770methyl jasmonateiTRAQATG8ATG18aautophagyROS<i>Euphorbia kansui</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoai Fang Xiangyu Yao Yue Zhang Zheni Tian Meng Wang Peng Li Xia Cai |
spellingShingle |
Xiaoai Fang Xiangyu Yao Yue Zhang Zheni Tian Meng Wang Peng Li Xia Cai iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L. International Journal of Molecular Sciences methyl jasmonate iTRAQ ATG8 ATG18a autophagy ROS <i>Euphorbia kansui</i> |
author_facet |
Xiaoai Fang Xiangyu Yao Yue Zhang Zheni Tian Meng Wang Peng Li Xia Cai |
author_sort |
Xiaoai Fang |
title |
iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L. |
title_short |
iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L. |
title_full |
iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L. |
title_fullStr |
iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L. |
title_full_unstemmed |
iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of <i>Euphorbia kansui</i> L. |
title_sort |
itraq-based proteomics analysis of autophagy-mediated responses against meja in laticifers of <i>euphorbia kansui</i> l. |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-08-01 |
description |
Autophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce a variety of metabolites. In plants, little is known about autophagy-mediated responses against MeJA. In this study, we used high-throughput comparative proteomics to identify proteins of latex in the laticifers. The isobaric tags for relative and absolute quantification (iTRAQ) MS/MS proteomics were performed, and 298 proteins among MeJA treated groups and the control group of <i>Euphorbia kansui</i> were identified. It is interesting to note that 29 significant differentially expressed proteins were identified and their associations with autophagy and ROS pathway were verified for several selected proteins as follows: α-L-fucosidase, β-galactosidase, cysteine proteinase, and Cu/Zn superoxide dismutase. Quantitative real-time PCR analysis of the selected genes confirmed the fact that MeJA might enhance the expression of some genes related to autophagy. The western blotting and immunofluorescence results of ATG8 and ATG18a which are two important proteins for the formation of autophagosomes also demonstrated that MeJA could promote autophagy at the protein level. Using the electron microscope, we observed an increase in autophagosomes after MeJA treatment. These results indicated that MeJA might promote autophagy in <i>E. kansui</i> laticifers; and it was speculated that MeJA mediated autophagy through two possible ways: the increase of ROS induces ATG8 accumulation and then aotophagosome formation, and MeJA promotes ATG18 accumulation and then autophagosome formation. Taken together, our results provide several novel insights for understanding the mechanism between autophagy and MeJA treatment. However, the specific mechanism remains to be further studied in the future. |
topic |
methyl jasmonate iTRAQ ATG8 ATG18a autophagy ROS <i>Euphorbia kansui</i> |
url |
https://www.mdpi.com/1422-0067/20/15/3770 |
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