Thioalbamide, A Thioamidated Peptide from <i>Amycolatopsis alba</i>, Affects Tumor Growth and Stemness by Inducing Metabolic Dysfunction and Oxidative Stress
Thioalbamide, a thioamidated peptide biosynthesized by <i>Amycolatopsis alba</i>, is a thioviridamide-like molecule, and is part of a family of natural products representing a focus of biotechnological and pharmaceutical research in recent years due to their potent anti-proliferative and...
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doaj-aa988c52bca84a53bef44feb3469314f2020-11-24T21:51:05ZengMDPI AGCells2073-44092019-11-01811140810.3390/cells8111408cells8111408Thioalbamide, A Thioamidated Peptide from <i>Amycolatopsis alba</i>, Affects Tumor Growth and Stemness by Inducing Metabolic Dysfunction and Oxidative StressLuca Frattaruolo0Marco Fiorillo1Matteo Brindisi2Rosita Curcio3Vincenza Dolce4Rodney Lacret5Andrew W. Truman6Federica Sotgia7Michael P. Lisanti8Anna Rita Cappello9Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Via P. Bucci, 87036 Rende (CS), ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, Via P. Bucci, 87036 Rende (CS), ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, Via P. Bucci, 87036 Rende (CS), ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, Via P. Bucci, 87036 Rende (CS), ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, Via P. Bucci, 87036 Rende (CS), ItalyDepartment of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UKDepartment of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UKTranslational Medicine, School of Environment and Life Sciences, Biomedical Research Centre (BRC), University of Salford, Greater Manchester M5 4WT, UKTranslational Medicine, School of Environment and Life Sciences, Biomedical Research Centre (BRC), University of Salford, Greater Manchester M5 4WT, UKDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, Via P. Bucci, 87036 Rende (CS), ItalyThioalbamide, a thioamidated peptide biosynthesized by <i>Amycolatopsis alba</i>, is a thioviridamide-like molecule, and is part of a family of natural products representing a focus of biotechnological and pharmaceutical research in recent years due to their potent anti-proliferative and cytotoxic activities on malignant cells. Despite the high antitumor potential observed at nanomolar concentrations, the mechanisms underlying thioalbamide activity are still not known. In this work, the cellular effects induced by thioalbamide treatment on breast cancer cell lines were evaluated for the first time, highlighting the ability of this microbial natural peptide to induce mitochondrial dysfunction, oxidative stress, and apoptotic cell death. Furthermore, we demonstrate that thioalbamide can inhibit the propagation of cancer stem-like cells, which are strongly dependent on mitochondrial function and are responsible for chemotherapy resistance, metastasis, and tumor recurrence.https://www.mdpi.com/2073-4409/8/11/1408thioviridamide-like moleculesrippsnatural productsanticancer agentcancer stem cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luca Frattaruolo Marco Fiorillo Matteo Brindisi Rosita Curcio Vincenza Dolce Rodney Lacret Andrew W. Truman Federica Sotgia Michael P. Lisanti Anna Rita Cappello |
spellingShingle |
Luca Frattaruolo Marco Fiorillo Matteo Brindisi Rosita Curcio Vincenza Dolce Rodney Lacret Andrew W. Truman Federica Sotgia Michael P. Lisanti Anna Rita Cappello Thioalbamide, A Thioamidated Peptide from <i>Amycolatopsis alba</i>, Affects Tumor Growth and Stemness by Inducing Metabolic Dysfunction and Oxidative Stress Cells thioviridamide-like molecules ripps natural products anticancer agent cancer stem cells |
author_facet |
Luca Frattaruolo Marco Fiorillo Matteo Brindisi Rosita Curcio Vincenza Dolce Rodney Lacret Andrew W. Truman Federica Sotgia Michael P. Lisanti Anna Rita Cappello |
author_sort |
Luca Frattaruolo |
title |
Thioalbamide, A Thioamidated Peptide from <i>Amycolatopsis alba</i>, Affects Tumor Growth and Stemness by Inducing Metabolic Dysfunction and Oxidative Stress |
title_short |
Thioalbamide, A Thioamidated Peptide from <i>Amycolatopsis alba</i>, Affects Tumor Growth and Stemness by Inducing Metabolic Dysfunction and Oxidative Stress |
title_full |
Thioalbamide, A Thioamidated Peptide from <i>Amycolatopsis alba</i>, Affects Tumor Growth and Stemness by Inducing Metabolic Dysfunction and Oxidative Stress |
title_fullStr |
Thioalbamide, A Thioamidated Peptide from <i>Amycolatopsis alba</i>, Affects Tumor Growth and Stemness by Inducing Metabolic Dysfunction and Oxidative Stress |
title_full_unstemmed |
Thioalbamide, A Thioamidated Peptide from <i>Amycolatopsis alba</i>, Affects Tumor Growth and Stemness by Inducing Metabolic Dysfunction and Oxidative Stress |
title_sort |
thioalbamide, a thioamidated peptide from <i>amycolatopsis alba</i>, affects tumor growth and stemness by inducing metabolic dysfunction and oxidative stress |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2019-11-01 |
description |
Thioalbamide, a thioamidated peptide biosynthesized by <i>Amycolatopsis alba</i>, is a thioviridamide-like molecule, and is part of a family of natural products representing a focus of biotechnological and pharmaceutical research in recent years due to their potent anti-proliferative and cytotoxic activities on malignant cells. Despite the high antitumor potential observed at nanomolar concentrations, the mechanisms underlying thioalbamide activity are still not known. In this work, the cellular effects induced by thioalbamide treatment on breast cancer cell lines were evaluated for the first time, highlighting the ability of this microbial natural peptide to induce mitochondrial dysfunction, oxidative stress, and apoptotic cell death. Furthermore, we demonstrate that thioalbamide can inhibit the propagation of cancer stem-like cells, which are strongly dependent on mitochondrial function and are responsible for chemotherapy resistance, metastasis, and tumor recurrence. |
topic |
thioviridamide-like molecules ripps natural products anticancer agent cancer stem cells |
url |
https://www.mdpi.com/2073-4409/8/11/1408 |
work_keys_str_mv |
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