Porous Alpha-Tricalcium Phosphate with Immobilized Basic Fibroblast Growth Factor Enhances Bone Regeneration in a Canine Mandibular Bone Defect Model
The effect of porous alpha-tricalcium phosphate (α-TCP) with immobilized basic fibroblast growth factor (bFGF) on bone regeneration was evaluated in a canine mandibular bone defect model. Identical bone defects were made in the canine mandible; six defects in each animal were filled with porous α-TC...
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doaj-aa99c30c477d470a8eaeebdb07f651fe2020-11-24T22:58:08ZengMDPI AGMaterials1996-19442016-10-0191085310.3390/ma9100853ma9100853Porous Alpha-Tricalcium Phosphate with Immobilized Basic Fibroblast Growth Factor Enhances Bone Regeneration in a Canine Mandibular Bone Defect ModelNobuhiro Kobayashi0Yoshiya Hashimoto1Akihisa Otaka2Tetsuji Yamaoka3Shosuke Morita4First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Hirakata 5731121, JapanDepartment of Biomaterials, Osaka Dental University; Hirakata 5731121, JapanDepartment of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita 5658565, JapanDepartment of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita 5658565, JapanFirst Department of Oral and Maxillofacial Surgery, Osaka Dental University, Hirakata 5731121, JapanThe effect of porous alpha-tricalcium phosphate (α-TCP) with immobilized basic fibroblast growth factor (bFGF) on bone regeneration was evaluated in a canine mandibular bone defect model. Identical bone defects were made in the canine mandible; six defects in each animal were filled with porous α-TCP with bFGF bound via heparin (bFGF group), whereas the other was filled with unmodified porous α-TCP (control group). Micro-computed tomography and histological evaluation were performed two, four and eight weeks after implantation. The bone mineral density of the bFGF group was higher than that of the control group at each time point (p < 0.05), and the bone mineral content of the bFGF group was higher than that of the control group at four and eight weeks (p < 0.05). Histological evaluation two weeks after implantation revealed that the porous α-TCP had degraded and bone had formed on the surface of α-TCP particles in the bFGF group. At eight weeks, continuous cortical bone with a Haversian structure covered the top of bone defects in the bFGF group. These findings demonstrate that porous α-TCP with immobilized bFGF can promote bone regeneration.http://www.mdpi.com/1996-1944/9/10/853alpha-tricalcium phosphatebasic fibroblast growth factorbone regeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nobuhiro Kobayashi Yoshiya Hashimoto Akihisa Otaka Tetsuji Yamaoka Shosuke Morita |
spellingShingle |
Nobuhiro Kobayashi Yoshiya Hashimoto Akihisa Otaka Tetsuji Yamaoka Shosuke Morita Porous Alpha-Tricalcium Phosphate with Immobilized Basic Fibroblast Growth Factor Enhances Bone Regeneration in a Canine Mandibular Bone Defect Model Materials alpha-tricalcium phosphate basic fibroblast growth factor bone regeneration |
author_facet |
Nobuhiro Kobayashi Yoshiya Hashimoto Akihisa Otaka Tetsuji Yamaoka Shosuke Morita |
author_sort |
Nobuhiro Kobayashi |
title |
Porous Alpha-Tricalcium Phosphate with Immobilized Basic Fibroblast Growth Factor Enhances Bone Regeneration in a Canine Mandibular Bone Defect Model |
title_short |
Porous Alpha-Tricalcium Phosphate with Immobilized Basic Fibroblast Growth Factor Enhances Bone Regeneration in a Canine Mandibular Bone Defect Model |
title_full |
Porous Alpha-Tricalcium Phosphate with Immobilized Basic Fibroblast Growth Factor Enhances Bone Regeneration in a Canine Mandibular Bone Defect Model |
title_fullStr |
Porous Alpha-Tricalcium Phosphate with Immobilized Basic Fibroblast Growth Factor Enhances Bone Regeneration in a Canine Mandibular Bone Defect Model |
title_full_unstemmed |
Porous Alpha-Tricalcium Phosphate with Immobilized Basic Fibroblast Growth Factor Enhances Bone Regeneration in a Canine Mandibular Bone Defect Model |
title_sort |
porous alpha-tricalcium phosphate with immobilized basic fibroblast growth factor enhances bone regeneration in a canine mandibular bone defect model |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2016-10-01 |
description |
The effect of porous alpha-tricalcium phosphate (α-TCP) with immobilized basic fibroblast growth factor (bFGF) on bone regeneration was evaluated in a canine mandibular bone defect model. Identical bone defects were made in the canine mandible; six defects in each animal were filled with porous α-TCP with bFGF bound via heparin (bFGF group), whereas the other was filled with unmodified porous α-TCP (control group). Micro-computed tomography and histological evaluation were performed two, four and eight weeks after implantation. The bone mineral density of the bFGF group was higher than that of the control group at each time point (p < 0.05), and the bone mineral content of the bFGF group was higher than that of the control group at four and eight weeks (p < 0.05). Histological evaluation two weeks after implantation revealed that the porous α-TCP had degraded and bone had formed on the surface of α-TCP particles in the bFGF group. At eight weeks, continuous cortical bone with a Haversian structure covered the top of bone defects in the bFGF group. These findings demonstrate that porous α-TCP with immobilized bFGF can promote bone regeneration. |
topic |
alpha-tricalcium phosphate basic fibroblast growth factor bone regeneration |
url |
http://www.mdpi.com/1996-1944/9/10/853 |
work_keys_str_mv |
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