Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541
Bordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three Fren...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2017-05-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | http://www.mdpi.com/1422-0067/18/5/1080 |
id |
doaj-aa9a991254ca47859bc0f7b288c08687 |
---|---|
record_format |
Article |
spelling |
doaj-aa9a991254ca47859bc0f7b288c086872020-11-25T02:43:09ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-05-01185108010.3390/ijms18051080ijms18051080Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541Valérie Bouchez0Sami AlBitar-Nehmé1Alexey Novikov2Nicole Guiso3Martine Caroff4Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, 25 rue du Dr Roux, 75724 Paris, FranceInstitute for integrative Biology of the Cell (I2BC), Commissariat à l’Energie Atomique (CEA), Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud, Université Paris-Saclay, 91405 Orsay, FranceLPS-BioSciences, I2BC, Bâtiment 409, Université de Paris-Sud, 91405 Orsay, FranceInstitut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, 25 rue du Dr Roux, 75724 Paris, FranceInstitute for integrative Biology of the Cell (I2BC), Commissariat à l’Energie Atomique (CEA), Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud, Université Paris-Saclay, 91405 Orsay, FranceBordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French B. holmesii clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography–mass spectrometry (GC–MS), and matrix-assisted laser desorption ionization–mass spectrometry (MALDI–MS). The analyses revealed a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkages. Similar to B. avium, B. hinzii and B. trematum lipids A, the hydroxytetradecanoic acid at the C-2′ position are carrying in secondary linkage a 2-hydroxytetradecanoic acid residue resulting of post-traductional biosynthesis modifications. The three clinical isolates displayed characteristic structural traits compared to the ATCC 51541 reference strain: the lipid A phosphate groups are more or less modified with glucosamine in the isolates and reference strain, but the presence of 10:0(3-OH) is only observed in the isolates. This trait was only described in B. pertussis and B. parapertussis strains, as well as in B. petrii isolates by the past. The genetic bases for most of the key structural elements of lipid A were analyzed and supported the structural data.http://www.mdpi.com/1422-0067/18/5/1080BordetellaeBordetella holmesiiendotoxinlipid Astructuremass spectrometrygenomic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Valérie Bouchez Sami AlBitar-Nehmé Alexey Novikov Nicole Guiso Martine Caroff |
spellingShingle |
Valérie Bouchez Sami AlBitar-Nehmé Alexey Novikov Nicole Guiso Martine Caroff Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541 International Journal of Molecular Sciences Bordetellae Bordetella holmesii endotoxin lipid A structure mass spectrometry genomic |
author_facet |
Valérie Bouchez Sami AlBitar-Nehmé Alexey Novikov Nicole Guiso Martine Caroff |
author_sort |
Valérie Bouchez |
title |
Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541 |
title_short |
Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541 |
title_full |
Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541 |
title_fullStr |
Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541 |
title_full_unstemmed |
Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541 |
title_sort |
bordetella holmesii: lipid a structures and corresponding genomic sequences comparison in three clinical isolates and the reference strain atcc 51541 |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-05-01 |
description |
Bordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French B. holmesii clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography–mass spectrometry (GC–MS), and matrix-assisted laser desorption ionization–mass spectrometry (MALDI–MS). The analyses revealed a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkages. Similar to B. avium, B. hinzii and B. trematum lipids A, the hydroxytetradecanoic acid at the C-2′ position are carrying in secondary linkage a 2-hydroxytetradecanoic acid residue resulting of post-traductional biosynthesis modifications. The three clinical isolates displayed characteristic structural traits compared to the ATCC 51541 reference strain: the lipid A phosphate groups are more or less modified with glucosamine in the isolates and reference strain, but the presence of 10:0(3-OH) is only observed in the isolates. This trait was only described in B. pertussis and B. parapertussis strains, as well as in B. petrii isolates by the past. The genetic bases for most of the key structural elements of lipid A were analyzed and supported the structural data. |
topic |
Bordetellae Bordetella holmesii endotoxin lipid A structure mass spectrometry genomic |
url |
http://www.mdpi.com/1422-0067/18/5/1080 |
work_keys_str_mv |
AT valeriebouchez bordetellaholmesiilipidastructuresandcorrespondinggenomicsequencescomparisoninthreeclinicalisolatesandthereferencestrainatcc51541 AT samialbitarnehme bordetellaholmesiilipidastructuresandcorrespondinggenomicsequencescomparisoninthreeclinicalisolatesandthereferencestrainatcc51541 AT alexeynovikov bordetellaholmesiilipidastructuresandcorrespondinggenomicsequencescomparisoninthreeclinicalisolatesandthereferencestrainatcc51541 AT nicoleguiso bordetellaholmesiilipidastructuresandcorrespondinggenomicsequencescomparisoninthreeclinicalisolatesandthereferencestrainatcc51541 AT martinecaroff bordetellaholmesiilipidastructuresandcorrespondinggenomicsequencescomparisoninthreeclinicalisolatesandthereferencestrainatcc51541 |
_version_ |
1724771220348469248 |