Occult HBV Infection in Immunized Neonates Born to HBsAg-Positive Mothers: A Prospective and Follow-Up Study.
Occult HBV infection (OBI) has been reported in infants born to HBsAg-positive mothers despite immunization. This study aims to determine the maintenance of this status in a prospective birth cohort.A total of 158 neonates born to HBsAg-positive mothers were enrolled. All received passive-active imm...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC5106040?pdf=render |
id |
doaj-aaa2579f0cd8451eb23e6da7f24255f9 |
---|---|
record_format |
Article |
spelling |
doaj-aaa2579f0cd8451eb23e6da7f24255f92020-11-25T01:49:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011111e016631710.1371/journal.pone.0166317Occult HBV Infection in Immunized Neonates Born to HBsAg-Positive Mothers: A Prospective and Follow-Up Study.Ying LuYa-Lin LiuJing-Jing NieXiao-Feng LiangLing YanFu-Zhen WangXiang-Jun ZhaiJian-Xun LiuFeng-Cai ZhuZhan-Jun ChangJie LiOccult HBV infection (OBI) has been reported in infants born to HBsAg-positive mothers despite immunization. This study aims to determine the maintenance of this status in a prospective birth cohort.A total of 158 neonates born to HBsAg-positive mothers were enrolled. All received passive-active immunization against HBV according to a 0-1-6 schedule. Sera were collected at 7 months of age. Those diagnosed with OBI were serially followed up at 12, 24 and 36 months of age. HBV serological markers were determined by Abbott i2000 system. HBV DNA was quantitated by Abbott m2000 system. Standard PCR followed by direct sequencing were applied for mother-child HBV pairs. Homology and phylogenetic comparisons were done by BLAST and Mega 5.All the 158 neonates were HBsAg-negative and anti-HBs-positive at 7 months of age, and 32 (20.3%) of them were diagnosed with OBI, with a median HBV DNA level of 1.97 (1.20-3.71) log IU/mL. Of them, HBV DNA was positive in 25.0%, 21.9% and 7.7% at 12, 24 and 36 months of age, respectively. HBV DNA disappeared at one of the follow-up points in 31 neonates, however, rebounded to low levels in 6 of them thereafter. HBV DNA persisted at low levels during follow-ups in the other one neonate apart from the above 31. All remained negative for HBsAg. Only two (6.3%) neonates were positive for anti-HBc after 24 months of age. HBV showed close homology and phylogenetic relationships for mother-child pairs. S-escape mutant, G145R, was not discovered. The first vaccine dose within 6 hours of birth significantly reduced the occurrence of OBI (59.4% vs. 83.3%, p = 0.003).HBV may be controlled in immunized neonates of HBsAg-positive mothers, after being diagnosed with OBI. Timely vaccination against HBV may provide the utmost protection. Long-term and close monitorings are needed.http://europepmc.org/articles/PMC5106040?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying Lu Ya-Lin Liu Jing-Jing Nie Xiao-Feng Liang Ling Yan Fu-Zhen Wang Xiang-Jun Zhai Jian-Xun Liu Feng-Cai Zhu Zhan-Jun Chang Jie Li |
spellingShingle |
Ying Lu Ya-Lin Liu Jing-Jing Nie Xiao-Feng Liang Ling Yan Fu-Zhen Wang Xiang-Jun Zhai Jian-Xun Liu Feng-Cai Zhu Zhan-Jun Chang Jie Li Occult HBV Infection in Immunized Neonates Born to HBsAg-Positive Mothers: A Prospective and Follow-Up Study. PLoS ONE |
author_facet |
Ying Lu Ya-Lin Liu Jing-Jing Nie Xiao-Feng Liang Ling Yan Fu-Zhen Wang Xiang-Jun Zhai Jian-Xun Liu Feng-Cai Zhu Zhan-Jun Chang Jie Li |
author_sort |
Ying Lu |
title |
Occult HBV Infection in Immunized Neonates Born to HBsAg-Positive Mothers: A Prospective and Follow-Up Study. |
title_short |
Occult HBV Infection in Immunized Neonates Born to HBsAg-Positive Mothers: A Prospective and Follow-Up Study. |
title_full |
Occult HBV Infection in Immunized Neonates Born to HBsAg-Positive Mothers: A Prospective and Follow-Up Study. |
title_fullStr |
Occult HBV Infection in Immunized Neonates Born to HBsAg-Positive Mothers: A Prospective and Follow-Up Study. |
title_full_unstemmed |
Occult HBV Infection in Immunized Neonates Born to HBsAg-Positive Mothers: A Prospective and Follow-Up Study. |
title_sort |
occult hbv infection in immunized neonates born to hbsag-positive mothers: a prospective and follow-up study. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
Occult HBV infection (OBI) has been reported in infants born to HBsAg-positive mothers despite immunization. This study aims to determine the maintenance of this status in a prospective birth cohort.A total of 158 neonates born to HBsAg-positive mothers were enrolled. All received passive-active immunization against HBV according to a 0-1-6 schedule. Sera were collected at 7 months of age. Those diagnosed with OBI were serially followed up at 12, 24 and 36 months of age. HBV serological markers were determined by Abbott i2000 system. HBV DNA was quantitated by Abbott m2000 system. Standard PCR followed by direct sequencing were applied for mother-child HBV pairs. Homology and phylogenetic comparisons were done by BLAST and Mega 5.All the 158 neonates were HBsAg-negative and anti-HBs-positive at 7 months of age, and 32 (20.3%) of them were diagnosed with OBI, with a median HBV DNA level of 1.97 (1.20-3.71) log IU/mL. Of them, HBV DNA was positive in 25.0%, 21.9% and 7.7% at 12, 24 and 36 months of age, respectively. HBV DNA disappeared at one of the follow-up points in 31 neonates, however, rebounded to low levels in 6 of them thereafter. HBV DNA persisted at low levels during follow-ups in the other one neonate apart from the above 31. All remained negative for HBsAg. Only two (6.3%) neonates were positive for anti-HBc after 24 months of age. HBV showed close homology and phylogenetic relationships for mother-child pairs. S-escape mutant, G145R, was not discovered. The first vaccine dose within 6 hours of birth significantly reduced the occurrence of OBI (59.4% vs. 83.3%, p = 0.003).HBV may be controlled in immunized neonates of HBsAg-positive mothers, after being diagnosed with OBI. Timely vaccination against HBV may provide the utmost protection. Long-term and close monitorings are needed. |
url |
http://europepmc.org/articles/PMC5106040?pdf=render |
work_keys_str_mv |
AT yinglu occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT yalinliu occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT jingjingnie occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT xiaofengliang occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT lingyan occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT fuzhenwang occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT xiangjunzhai occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT jianxunliu occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT fengcaizhu occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT zhanjunchang occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy AT jieli occulthbvinfectioninimmunizedneonatesborntohbsagpositivemothersaprospectiveandfollowupstudy |
_version_ |
1725005126877315072 |